Aloe Vera

No. Records Request

1: 324 ALOE

MEDLINE EXPRESS (R) 1984-1990 1 of 324

TI: [Protective effect of extracts from Aloe vera L. var. chinensis (Haw.) Berg. on experimental hepatic lesions and a primary clinical study on the injection of in patients with hepatitis]

AU: Fan-YJ; Li-M; Yang-WL; Qin-L; Zou-J

SO: Chung-Kuo-Chung-Yao-Tsa-Chih. 1989 Dec; 14(12): 746-8

ISSN: 1001-5302

LA: CHINESE; NON-ENGLISH

AB: The injection(10-15 ml/kg/d, ip x 4), total glycoside (125-225 mg/kg/d, ip x (3-4); 600 mg/kg/d, ig x 3) and crystal III (120 mg/kg/d, ip x 4) of Aloe vera var. chinensis were found to be effective in lowering the elevated sGPT induced by CCl4, thioacetamide and D-aminogalactose in mice or rats. It was also observed that these agents could protect hepatic cells from the CCl4-induced injury. When dogs were given in with Aloe injection of 0.1 ml/kg/d x 180, no toxicity was noted. The total effective sGPT-lowering rate of Aloe injection on 38 patients of chronic hepatitis with positive HBsAg was 86.8%.

AN: 90248098

MEDLINE EXPRESS (R) 1984-1990 2 of 324

TI: The role of topical agents in the healing of full-thickness wounds.

AU: Watcher-MA; Wheeland-RG

AD: Department of Dermatology, University of California, Sacramento 95816.

SO: J-Dermatol-Surg-Oncol. 1989 Nov; 15(11): 1188-95

This title is owned by this library

ISSN: 0148-0812

LA: ENGLISH

AB: Eight topical agents in current use were studied for their effects on wound contraction and rate of reepithelialization of full-thickness excisions using a porcine animal model. The following agents were applied daily for a period of 27 days: scarlet red ointment, benzoyl peroxide lotion, bacitracin ointment, silver sulfadiazine cream, aloe vera gel, tretinoin cream, capsaicin cream, and mupirocin ointment. The rate of reepithelialization was significantly enhanced by treatment with capsaicin, bacitracin, silver sulfadiazine, and scarlet red, and was markedly retarded by treatment with tretinoin. Wound contraction was significantly retarded by mupirocin, bacitracin, and silver sulfadizine. Knowledge of the effects of topical agents on various aspects of healing allows the clinician to choose the most appropriate material to use in a given clinical situation to optimize the healing process and produce the best final result.

AN: 90037909

MEDLINE EXPRESS (R) 1984-1990 3 of 324

TI: Adrenalectomy decreases nerve growth factor in young adult rat hippocampus.

AU: Aloe-L

AD: Istituto di Neurobiologia, Consiglio Nazionale della Ricerca, Rome, Italy.

SO: Proc-Natl-Acad-Sci-U-S-A. 1989 Jul; 86(14): 5636-40

This title is owned by this library

ISSN: 0027-8424

LA: ENGLISH

AB: The effect of adrenalectomy on the level of nerve growth factor (NGF) in the hippocampus and on the distribution of choline acetyltransferase immunoreactivity in forebrain cholinergic neurons of developing rats was studied. Biological and immunohistochemical determinations indicated that in 40-day-old rats, adrenalectomy reduced the NGF level in the hippocampus and the choline acetyltransferase immunoreactivity in the septal lateral bands. Furthermore, autoradiographic studies showed that adrenalectomy causes changes in the distribution and expression of NGF receptors in the hippocampus. These results suggest that adrenal hormones are involved in the regulation of the NGF level in the hippocampus and of NGF receptors in the septum.

AN: 89315852

MEDLINE EXPRESS (R) 1984-1990 4 of 324

TI: Effect of water extracts of aloe and some herbs in decreasing blood ethanol concentration in rats. II.

AU: Sakai-K; Saitoh-Y; Ikawa-C; Nishihata-T

SO: Chem-Pharm-Bull-Tokyo. 1989 Jan; 37(1): 155-9

This title is owned by this library

ISSN: 0009-2363

LA: ENGLISH

AB: Oral administration of ethanol to rats at a dose of 3 g/kg decreased alcohol dehydrogenase (ADH) activity and metabolism of lactate to pyruvate in the liver. The effects of water extracts of Aloe and some other herbs on blood ethanol concentration and on ADH activity in liver cytosol were examined. The water extracts of these herbs caused a faster elimination of ethanol from blood of normal rats when administered orally 30 min before oral administration of ethanol. The rapid elimination of ethanol seems to be due to a protection of ADH activity and the supply of nicotinamide dinucleotide, both of which are reduced by high ethanol concentration. The effects of ethanol in decreasing the enzyme activities relating to its own metabolism occur when high concentrations of ethanol pass through the liver, and thus may primarily appear during the absorption of alcohol from the gastrointestinal tract, when portal concentration of ethanol are very high.

AN: 89249527

MEDLINE EXPRESS (R) 1984-1990 5 of 324

TI: [Evaluation of the cationic-lysosomal test in patients with pneumonia against a background of therapy]

TO: Otsenka kationno-lizosomal'nogo testa u bol'nykh pnevmoniei na fone terapii.

AU: Domashenko-ON; Sotnik-IuP

SO: Lab-Delo. 1989(5): 15-7

This title is owned by this library

ISSN: 0023-6748

LA: RUSSIAN; NON-ENGLISH

AB: The authors' findings evidence that measurements of cationic protein levels is a sufficiently informative test in the complex of investigations that help predict the course of acute pneumonia and the therapeutic efficacy of drugs stimulating the nonspecific resistance of the body. Combined therapy with the use of methyluracil and eleutherococcus is more effective for pneumonia patients than the complex including aloe and antibacterial and symptomatic agents alone.

AN: 89312532

MEDLINE EXPRESS (R) 1984-1990 6 of 324

TI: [A comprehensive assessment of the phagocytic activity of the blood neutrophils in patients with rubromycosis treated with griseofulvin and nizoral]

TO: Kompleksnaia otsenka fagotsitarnoi aktivnosti neitrofilov krovi u bol'nykh rubrofitiei, lechennykh grizeoful'vinom i nizoralom.

AU: Belukha-UK; Bidrat-MS; Luk'ianova-AS

SO: Vestn-Dermatol-Venerol. 1989(1): 50-2

ISSN: 0042-4609

LA: RUSSIAN; NON-ENGLISH

AB: The neutrophilic phagocytic activity (absorption and enzymic bactericidal) is intensified in rubromycosis patients treated with nizoral vs. those administered griseofulvin. it is advisable to combine griseofulvin therapy with biogenic stimulants (pyrogenal, aloe, fiBS, vitreous body).

AN: 89243798

MEDLINE EXPRESS (R) 1984-1990 7 of 324

TI: [Acute myeloid leukemia: prognostic factors]

TO: Leucemia mieloide acuta: fattori prognostici.

AU: Petti-MC; Aloe-Spiriti-MA; Latagliata-R; Spadea-A; Tirindelli-MC; Vegna-ML

SO: Haematologica. 1989 Oct; 74(5 Suppl): 380-91

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ISSN: 0390-6078

LA: ITALIAN; NON-ENGLISH

AN: 90077150

MEDLINE EXPRESS (R) 1984-1990 8 of 324

TI: Adult acute lymphoblastic leukemia: description and analysis of long-term survivors. A retrospective study.

AU: Giona-F; Mazzucconi-MG; Aloe-Spiriti-MA; Defazio-D; De-Luca-AM; Ferrazza-G; Martinelli-E; Mandelli-F

SO: Haematologica. 1989 Sep-Oct; 74(5): 475-80

This title is owned by this library

ISSN: 0390-6078

LA: ENGLISH

AB: This retrospective study, including 118 patients with acute lymphoblastic leukemia (ALL) aged greater than 15 years, with a minimum follow-up of 6 years, was aimed at defining potentially "cured" adults with ALL. At present, 21 out of 92 patients who achieved complete remission (CR) are long survivors: 16 in first CR, off-therapy; 4 in 2nd CR (3 off-therapy); 1 in 3rd CR, on treatment. On the basis of available data, we tried to identify factors at diagnosis which might predict long-term survival: white blood cell (WBC) count on admission was the only significant prognostic factor for overall survival (p = 0.0002) and first CR duration (p = 0.0005). The survival hazard rate (risk of death from acute leukemia per day) reaches 0 between 8 and 9 years from diagnosis. From our data we can identify two groups of ALL long-term survivors: the first includes 16 patients in 1st continuous CR (CCR), 12 of whom in CCR for over 8 years may be considered "cured"; the second group comprises 5 patients, relapsing once or twice, alive in 2nd or 3rd CR.

AN: 90060996

MEDLINE EXPRESS (R) 1984-1990 9 of 324

TI: Intensification in post-remission treatment of adult acute non lymphocytic leukemia.

AU: Petti-MC; Aloe-Spiriti-MA; Carella-AM; Fioritoni-G; Resegotti-L; Rizzoli-V; Tabilio-A; Visani-G; Vegna-ML; Mandelli-F

SO: Haematologica. 1989 May-Jun; 74(3): 267-71

This title is owned by this library

ISSN: 0390-6078

LA: ENGLISH

AB: In an attempt to reduce the risk of leukemic relapse, different post-remission intensifications based on high-dose Ara-C (HiDAC) and autologous bone marrow transplantation (ABMT) were evaluated in patients with acute non lymphocytic leukemia in first remission and compared as to response and toxicity. Between September, 1985 and May, 1987, 34 patients in complete remission were eligible for our study. Induction therapy consisted of one or two courses of daunorubicin (DNR) and Ara-C (schedule 3 + 7). Fourteen patients receiving intensive post-remission chemotherapy with DNR + Ara-C (schedule 2 + 5), HiDAC + DNR, and ABMT following pretransplant BAVC conditioning entered the first pilot study. A high toxicity was observed and only 5 of them completed the full treatment plan. Thus the second pilot study used a single post-remission intensive course with HiDAC + m-AMSA and ABMT following cyclophosphamide plus TBI or BAVC. This approach was more feasible. The preliminary results show the usefulness of intensive post-remission therapy: in fact, all patients but one who completed the treatment program are still in continuous complete remission. A large number of patients and a longer follow-up are required to draw final conclusions.

AN: 90060972

MEDLINE EXPRESS (R) 1984-1990 10 of 324

TI: Epidemiologic survey on lung cancer with respect to cigarette smoking and plant diet.

AU: Sakai-R

AD: Department of Epidemiology, School of Health Sciences, Ryukyu University, Okinawa.

SO: Jpn-J-Cancer-Res. 1989 Jun; 80(6): 513-20

This title is owned by this library

ISSN: 0910-5050

LA: ENGLISH

AB: This case-control study of lung cancer was based on a cross-sectional questionnaire survey of inpatients at 5 general hospitals in Okinawa, Japan, from 1982 to 1987. The purpose of the study was to clarify the relations of lung cancer to cigarette smoking and plant diet. Ingestion frequencies of 17 major dietary plants and/or herbs were obtained by means of a questionnaire interview. As eligible subjects for a case-control analysis, there were 673 respondents aged over 30 years with clear smoking history, age, sex and diagnosis. Psychiatric patients were excluded. Odds ratios of newly diagnosed lung cancer were calculated by the Mantel-Haenszel procedure. A pair consisted of a case and two controls which were selected randomly by using multivariate caliper matching. Sixty-four pairs matched for age (+/- 5) and sex showed a significantly high odds ratio of 2.9 (P less than 0.0005). However, three male groups who were categorized by the number of cigarettes smoked did not exhibit dose-dependency of lung cancer on smoking. Lung cancer was more prevalent in ex-smokers than in current smokers. Case-control analyses by male generations revealed that lung cancer incidence was age-dependent, and there was a clear dose-response relationship between smoking and lung cancer in males in their sixties. A case-control analysis of each of 17 edible plants based on 44 pairs who were matched for age (+/- 5), sex and smoking history demonstrated that the odds ratio of aloe (Aloe arborescens Mill var. natalensis Berger) was 0.5 (P less than 0.1), suggesting that the aloe may prevent human carcinogenesis at various sites.

AN: 89340133

MEDLINE EXPRESS (R) 1984-1990 11 of 324

TI: [Prospective study of the immunomodulator properties of i.m. administered "ALVA" extract in patients with solid tumors under a course of chemical immunosuppressive therapy]

TO: Etude prospective des proprietes immunomodulatrices de l'extrait "ALVA" utilise en "I.M." chez des patients atteints de tumeurs solides et en cours de therapie immunosuppressive chimique.

AU: Michel-P; Pignon-T; Ralamboranto-L; Randrianandraina-S; Ratovonarivo-A; Coulanges-P

AD: Institut Pasteur Antannerivo.

SO: Arch-Inst-Pasteur-Madagascar. 1989; 56(1): 253-9

This title is owned by this library

ISSN: 0020-2495

LA: FRENCH; NON-ENGLISH

AN: 90225894

MEDLINE EXPRESS (R) 1984-1990 12 of 324

TI: [Effects of aloe extracts, aloctin A, on gastric secretion and on experimental gastric lesions in rats]

AU: Saito-H; Imanishi-K; Okabe-S

SO: Yakugaku-Zasshi. 1989 May; 109(5): 335-9

ISSN: 0031-6903

LA: JAPANESE; NON-ENGLISH

AB: Effect of aloctin A, glycoprotein isolated from leaves of Aloe arborescens MILL, on gastric secretion and on acute gastric lesions in rats were examined. Aloctin A given intravenously dose-dependently inhibited the volume of gastric juice, acid and pepsin output in pylorus-ligated rats. Aloctin A given intravenously significantly inhibited the development of Shay ulcers and indomethacin-induced gastric lesions in rats. It also inhibited water-immersion stress lesions induced in pylorus-ligated rats.

AN: 90172180

MEDLINE EXPRESS (R) 1984-1990 13 of 324

TI: Non-epileptic myoclonus and mitochondrial encephalomyopathy.

AU: Cukiert-A; Naylor-FG; Scapolan-HB; Vilela-MM; Aloe-FS; Siffert-JO; Tsanaclis-AM; Haddad-M; Machado-TC; Carvalho-Alegro-M; et-al

AD: Faculty of Medicine, University of Sao Paulo, Brasil.

SO: Arq-Neuropsiquiatr. 1989 Sep; 47(3): 346-51

This title is owned by this library

ISSN: 0004-282X

LA: ENGLISH

AB: Two brothers presented to us with a progressive myoclonic syndrome with slight cerebellar symptoms. Neurological examination disclosed moderate cerebellar signs and pale optic discs; asymmetric, asynchronous and arrhythmic myoclonus, an arrthesthesic deficit and no muscular weakness. EEG background activity was moderately slow with no irritative discharges. CT was normal in both cases. Intermittent photic stimulation increased the frequency of the myoclonic jerks, which became bilateral and synchronous, progressing to a generalized tonic-clonic seizure. EPs and MRI in one case were normal. Anticonvulsant drugs were ineffective. The diagnosis of mitochondrial encephalomyopathy was based on the finding, in muscle specimens, of thickened basement membranes with myofibrillary degeneration and increased number of mitochondria peripherally distributed and with a dense granular matrix and some vacuoles. The clinical and EEG data suggest a subcortical origin for this type of myoclonic syndrome.

AN: 90147107

MEDLINE EXPRESS (R) 1984-1990 14 of 324

TI: An anti-complementary polysaccharide with immunological adjuvant activity from the leaf parenchyma gel of Aloe vera.

AU: t'Hart-LA; van-den-Berg-AJ; Kuis-L; van-Dijk-H; Labadie-RP

SO: Planta-Med. 1989 Dec; 55(6): 509-12

This title is owned by this library

ISSN: 0032-0943

LA: ENGLISH

AB: The aim of the study is to develop new substances with immunomodulatory activity. To this end, extracts from plants used in traditional medicine are used as starting material. This study deals with the mucilagenous leaf-gel of Aloe vera which is well reputed for its therapeutical effect on inflammatory-based disorders. The purification of an aqueous gel-extract guided by inhibition of complement activity in HPS is described. Using anion-exchange and gel permeation chromatography a highly active polysaccharide fraction was isolated, that is present in the gel in various chain lengths. The polysaccharides consist of several monosaccharides of which mannose is dominant. The inhibition is based on alternative pathway activation, resulting in consumption of C3. With respect to their biological activity the polysaccharides inhibit the opsonization of zymosan in HPS and display adjuvant activity on specific antibody production and the induction of delayed type hypersensitivity in mice.

AN: 90139301

MEDLINE EXPRESS (R) 1984-1990 15 of 324

TI: Aloe vera does not affect cutaneous erythema and blood flow following ultraviolet B exposure.

AU: Crowell-J; Hilsenbeck-S; Penneys-N

AD: Department of Dermatology, University of Miami School of Medicine, Florida.

SO: Photodermatol. 1989 Oct; 6(5): 237-9

This title is owned by this library

ISSN: 0108-9684

LA: ENGLISH

AN: 90138468

MEDLINE EXPRESS (R) 1984-1990 16 of 324

TI: Wound healing. Oral and topical activity of Aloe vera.

AU: Davis-RH; Leitner-MG; Russo-JM; Byrne-ME

SO: J-Am-Podiatr-Med-Assoc. 1989 Nov; 79(11): 559-62

ISSN: 8750-7315

LA: ENGLISH

AB: The influence of Aloe vera, orally and topically, on wound healing was studied. Wounds were induced on both sides of the vertebral column of ICR mice using a biopsy punch. For the oral study, experimental animals received A. vera in their drinking water for 2 months, whereas the control animals received only water. In the topical study, experimental animals were given 25% A. vera in Eucerin cream topically. The control animals received cream only. A 62.5% reduction in wound diameter was noted in mice receiving 100 mg/kg/day oral A. vera and a 50.8% reduction was recorded in animals receiving topical 25% A. vera. These data suggest that A. vera is effective by both oral and topical routes of administration.

AN: 90112031

MEDLINE EXPRESS (R) 1984-1990 17 of 324

TI: NGF primed spleen cells injected in brain of developing rats differentiate into mast cells.

AU: Aloe-L; De-Simone-R

AD: Institute of Cell Biology, C.N.R., Rome, Italy.

SO: Int-J-Dev-Neurosci. 1989; 7(6): 565-73

This title is owned by this library

ISSN: 0736-5748

LA: ENGLISH

AB: The aim of this work was to study the topographic distribution and the morphological behaviour of nerve growth factor (NGF) primed spleen cells injected into the lateral ventricles of developing rat brain. Serial coronal brain sections showed that these transplanted cells acquire phenotypical characteristics similar to those of mast cells (MCs) and that they enhance local neovascularization. These results, together with the observation that these cells are located in proximity to the hippocampus, a brain tissue which contains one of the highest levels of NGF, provide a model for studying the relationship between NGF and MC differentiation and secretion.

AN: 90101992

MEDLINE EXPRESS (R) 1984-1990 18 of 324

TI: [Effect of drug preparations in vitro on the morphofunctional status of spermatozoa]

TO: Vliianie nekotorykh lekarstvennykh preparatov in vitro na morfofunktsional'noe sostoianie spermatozoonov.

AU: Israilov-SR

SO: Vrach-Delo. 1989 Aug(8): 52-4

ISSN: 0049-6804

LA: RUSSIAN; NON-ENGLISH

AB: The effect was studied of different drugs (aloe extract, penicillin, vitreous body and lidase) in 20 specimens of the ejaculate with established normozoospermia and in 20 specimens with oligozoospermia, asthenozoospermia and hypozoospermia. Experiments were carried out at +4 degrees C to -38 degrees C, every 2 hours for 72 hours. It was established that depending on the time, temperature and drugs there was an essential difference between the percent of mobility and ultrastructure of spermatozoa. The most favourable effect on spermatozoa was produced by temperature ranges from 11 to 18 degrees C, lidase and penicillin.

AN: 90070580

MEDLINE EXPRESS (R) 1984-1990 19 of 324

TI: [Chronic benign familial pemphigus]

TO: Khronicheskaia dobrokachestvennaia semeinaia puzyrchatka.

AU: Kovalev-VM; Tkachenko-LA; Krivenko-ZF; Stefanovskaia-LP

SO: Vestn-Dermatol-Venerol. 1989(8): 65-7

ISSN: 0042-4609

LA: RUSSIAN; NON-ENGLISH

AB: A female patient aged 50 is described, in whom candidiasis of the large folds has been erroneously diagnosed for 10 years; the diagnosis has not been confirmed by bacteriologic analysis, the therapy has been ineffective, the recurrences developed every year. The patient has developed symmetrical erythematous infiltrative areas in the armpits, groin, and in the folds under mammary glands, with solitary bullae up to 0.5-1.0 cm in diameter, with transparent contents, with thin flaccid covers and fragments thereof, as well as small pigmented vegetation. Basing on the histologic findings (suprabasal vesicles above the basal layer, acantholysis in the epidermis, and negligible lymphocytic infiltrate in the derma), Gougerot-Hailey-Hailey's disease has been diagnosed. The familial nature of the disease could not be established. Therapy with diphenhydramine, methyluracil, dimocifon, aloe extract, and aniline dyes, administered for 20 days, has resulted in clinical cure.

AN: 90051784

MEDLINE EXPRESS (R) 1984-1990 20 of 324

TI: Nerve growth factor mRNA and protein increase in hypothalamus in a mouse model of aggression.

AU: Spillantini-MG; Aloe-L; Alleva-E; De-Simone-R; Goedert-M; Levi-Montalcini-R

AD: Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom.

SO: Proc-Natl-Acad-Sci-U-S-A. 1989 Nov; 86(21): 8555-9

This title is owned by this library

ISSN: 0027-8424

LA: ENGLISH

AB: The effects of intermale aggressive behavior induced by social isolation on the level of nerve growth factor (NGF) mRNA and protein were investigated in central and peripheral mouse tissues. A large increase in NGF mRNA and protein was observed in hypothalamus, with no changes in cerebral cortex, hippocampus, and cerebellum. No change in NGF mRNA levels was found in heart, spleen, vas deferens, and submaxillary salivary gland. The cellular localization of NGF mRNA in the central nervous system was investigated by in situ hybridization. Numerous nerve cells were specifically labeled in preoptic and ventrolateral nuclei of the hypothalamus, as well as in the cornu ammonis region of the hippocampus and throughout all layers of the cerebral cortex, with the highest concentration in layer III. The present results firmly establish that nerve cells constitute the major source in NGF in the brain. They also open the way to understanding the regulation of NGF biosynthesis in the central nervous system.

AN: 90046836

MEDLINE EXPRESS (R) 1984-1990 21 of 324

TI: Processed Aloe vera administered topically inhibits inflammation.

AU: Davis-RH; Rosenthal-KY; Cesario-LR; Rouw-GA

SO: J-Am-Podiatr-Med-Assoc. 1989 Aug; 79(8): 395-7

ISSN: 8750-7315

LA: ENGLISH

AB: Aloe vera preparations were evaluated for topical anti-inflammatory activity using the croton oil-induced edema assay. The results show that small amounts of A. vera given topically will inhibit inflammation induced by a moderate amount of irritant. In general, the decolorized Aloe was more effective than the colorized Aloe (with anthraquinone). A 47.1% inhibition of inflammation was obtained by 5% decolorized irradiated Aloe. These results may be used as a baseline to assess the biologic activity of A. vera in the treatment of inflammation by podiatric physicians.

AN: 90040137

MEDLINE EXPRESS (R) 1984-1990 22 of 324

TI: [Rehabilitation of patients with ulcers under hospital conditions]

TO: Reabilitatsiia bol'nykh iazvennoi bolezn'iu v statsionarnykh usloviiakh.

AU: Kovalenko-MF; Dergachev-BP

SO: Voen-Med-Zh. 1989 Jun(6): 29-30

ISSN: 0026-9050

LA: RUSSIAN; NON-ENGLISH

AN: 90021263

MEDLINE EXPRESS (R) 1984-1990 23 of 324

TI: Anti-inflammatory activity of Aloe vera against a spectrum of irritants.

AU: Davis-RH; Leitner-MG; Russo-JM; Byrne-ME

SO: J-Am-Podiatr-Med-Assoc. 1989 Jun; 79(6): 263-76

ISSN: 8750-7315

LA: ENGLISH

AB: The authors have evaluated the spectrum of anti-inflammatory activity of A. vera in a number of models of inflammation in the hind paw of the experimental rat induced by kaolin, carrageenan, albumin, dextran, gelatin, and mustard. Croton oil was used in a topical model of inflammation to determine the oral activity and time-dependent dosing of A. vera. The authors found that A. vera was active in all models of inflammation. Of the various irritants tested, A. vera was especially active against gelatin-induced and kaolin-induced edema and, in contrast, had minimal activity when tested against dextran-induced edema. Oral activity of A. vera was demonstrated to be dependent on the presence of anthraquinones. The various irritant-induced edema models provided a broad spectrum of anti-inflammatory activity for A. vera.

AN: 89311023

MEDLINE EXPRESS (R) 1984-1990 24 of 324

TI: [Studies on chemical protectors against radiation. XXVI. Protective effect of various extracts on crude drugs on skin injury induced by X-irradiation]

AU: Sato-Y; Ohta-S; Sakurai-N; Shinoda-M

SO: Yakugaku-Zasshi. 1989 Feb; 109(2): 113-8

ISSN: 0031-6903

LA: JAPANESE; NON-ENGLISH

AB: The protective potency against skin injury on mice induced by X-irradiation was studied by use of 72 extracts of crude drugs. The protective potency was determined according to the degrees on skin injury after irradiation of 1100R, 30 k Vp soft X-ray. As a result of these study, 16 kinds of crude drugs such as Rosae Fructus, Aloe arborescens (Herba), Citri Leiocarpae Exocarpium, Schizonepetae Spica, Evodiae Fructus, Bupleuri Radix, Corni Fructus, Perillae Herba, Anemarrhenae Rhizoma, Menthae Herba, Trapae Fructus, Angelicae Dahuricae Radix, Sinomeni Caulis et Rhizoma, Ephedrae Herba, Acer nikoense (Cortex), Forsythiae Fructus, revealed protective potencies on skin injury.

AN: 89310978

MEDLINE EXPRESS (R) 1984-1990 25 of 324

TI: [Primary screening of the immunopharmacologic activity of Filatov tissue therapy preparations]

TO: Pervichnyi skrining immunofarmakologicheskoi aktivnosti preparatov tkanevoi terapii po V.P. Filatovu.

AU: Degtiarenko-TV; Ivanova-AS; Skvortsov-VIu; Masternak-TV; Larin-AS

SO: Oftalmol-Zh. 1989(1): 34-9

ISSN: 0030-0675

LA: RUSSIAN; NON-ENGLISH

AB: The paper presents results of primary assessment of immunopharmacologic activity of tissue preparations (peloidodistillate, FiBS, torfot, extract of aloe, placental extract) and the study of their influence on basic links of immune response (experimental investigations were carried out with mice-hybrids F1). It is found that the studied bioregulators possess immunomodulating action on primary humoral immune response and don't increase a hypersensitive reaction of a slowed type. The direction of immunotropic action of tissue preparations depends on the state of immunologic reactivity of the body and the kind of a preparation (variety of immunopharmacologic activity of the distillates groups and albumin preparations). It is expedient to work out indications to the usage of tissue preparations after V. P. Filatov to prevent and treat immunodeficient and immunopathologic conditions of the body.

AN: 89282099

MEDLINE EXPRESS (R) 1984-1990 26 of 324

TI: [Experience using antihypoxic drugs in the treatment of acute neurosensory hearing loss in children with meningitis]

TO: Opyt primeneniia antigipoksantov v lechenii ostroi neirosensornoi tugoukhosti pri meningitakh u detei.

AU: Ryndina-AM; Lin'kov-VI; Dadiomova-MA; Buriakova-AV

SO: Vestn-Otorinolaringol. 1989 Mar-Apr(2): 14-7

This title is owned by this library

ISSN: 0042-4668

LA: RUSSIAN; NON-ENGLISH

AB: This paper describes beneficial affects of the therapy of neurosensory hypoacusia that complicated such neuroinfectious diseases as meningococcemia, meningococcic meningitis and meningitides of other etiology using traditional drugs (ATP, aloe, B and C vitamins) in combination with the antihypoxic agent gutimine and its analog. Positive effects were recorded in 17 (81%) out of 21 children. It is indicated that the agent shows higher efficacy when used within the first weeks after the onset of the disease.

AN: 89268134

MEDLINE EXPRESS (R) 1984-1990 27 of 324

TI: Aloe vera and burn wound healing [letter]

AU: Kaufman-T; Newman-AR; Wexler-MR

SO: Plast-Reconstr-Surg. 1989 Jun; 83(6): 1075-6

This title is owned by this library

ISSN: 0032-1052

LA: ENGLISH

AN: 89265229

MEDLINE EXPRESS (R) 1984-1990 28 of 324

TI: Aloe vera and gibberellin. Anti-inflammatory activity in diabetes.

AU: Davis-RH; Maro-NP

SO: J-Am-Podiatr-Med-Assoc. 1989 Jan; 79(1): 24-6

ISSN: 8750-7315

LA: ENGLISH

AB: Aloe vera inhibits inflammation and adjuvant-induced arthritis. The authors' laboratory has shown that A. vera improves wound healing, which suggests that it does not act like an adrenal steroid. Diabetic animals were used in this study because of their poor wound healing and anti-inflammatory capabilities. The anti-inflammatory activity of A. vera and gibberellin was measured in streptozotocin-induced diabetic mice by measuring the inhibition of polymorphonuclear leukocyte infiltration into a site of gelatin-induced inflammation over a dose range of 2 to 100 mg/kg. Both Aloe and gibberellin similarly inhibited inflammation in a dose-response manner. These data tend to suggest that gibberellin or a gibberellin-like substance is an active anti-inflammatory component in A. vera.

AN: 89258381

MEDLINE EXPRESS (R) 1984-1990 29 of 324

TI: [Purification, glucose-6-phosphate dehydrogenase inhibition, and HPLC analysis of four 1,8-dihydroxyanthrones]

TO: Reindarstellung, Glucose-6-phosphat-Dehydrogenase-Hemmung und HPLC-Analytik von vier 1,8-Dihydroxyanthronen.

AU: Rychener-M; Steiger-W

SO: Pharm-Acta-Helv. 1989; 64(1): 8-15

This title is owned by this library

ISSN: 0031-6865

LA: GERMAN; NON-ENGLISH

AB: With regard to the examination of their antipsoriatic properties, four 1,8-dihydroxyanthrones were prepared in a purity greater than 99%: Dithranol (1, anthralin) by chromatographic purification, chrysarobin (2) either by work-up from commercial chrysarobin or by an oxidative cleavage (FeCl3) of aloin, followed by a reduction (SnCl2/HCl) of the produced anthraquinone derivative (6), aloe emodin anthrone (3) by the action of aquous sodium tetraborate on aloin and frangula emodin anthrone (4) by the SnCl2/HCl-reduction of frangula emodin (7) isolated from the dry extract of the bark of alder buckthorn. UV/VIS, IR, 1H-NMR and mass spectra confirmed the structures of the four anthrones which all distinctly inhibited G-6-P dehydrogenase (0.05 mmol, desactivation in 6 h). For clinical studies the anthrones were incorporated into white petrolatum with exclusion of oxygen (under Ar). The anthrone content of the preparations was determined by HPLC (LiChrosorb RP-18, 7 microns [280 x 4 mm]; 1.5 ml/min MeOH/water/HOAc [80:20:0.2]) after extraction with CH2Cl2 (tR = 1: 10.4 min, 2: 15.3 min, 3: 4.7 min, 4: 7.7 min).

AN: 89240828

MEDLINE EXPRESS (R) 1984-1990 30 of 324

TI: Aloe vera, salicylic acid, and aspirin for burns [letter]

AU: Frumkin-A

SO: Plast-Reconstr-Surg. 1989 Jan; 83(1): 196

This title is owned by this library

ISSN: 0032-1052

LA: ENGLISH

AN: 89071995

MEDLINE EXPRESS (R) 1984-1990 31 of 324

TI: Aloe vera for burns [letter]

AU: Kivett-WF

SO: Plast-Reconstr-Surg. 1989 Jan; 83(1): 195

This title is owned by this library

ISSN: 0032-1052

LA: ENGLISH

AN: 89071993

MEDLINE EXPRESS (R) 1984-1990 32 of 324

TI: Widespread dermatitis after topical treatment of chronic leg ulcers and stasis dermatitis.

AU: Hogan-DJ

AD: Department of Medicine, University of Saskatchewan, Saskatoon.

SO: CMAJ. 1988 Feb 15; 138(4): 336-8

ISSN: 0820-3946

LA: ENGLISH

AN: 88109174

MEDLINE EXPRESS (R) 1984-1990 33 of 324

TI: Two functionally and chemically distinct immunomodulatory compounds in the gel of Aloe vera.

AU: 't-Hart-LA; van-Enckevort-PH; van-Dijk-H; Zaat-R; de-Silva-KT; Labadie-RP

AD: Department of Chemical Pharmacy, Faculty of Pharmacy, State University of Utrecht, The Netherlands.

SO: J-Ethnopharmacol. 1988 May-Jun; 23(1): 61-71

This title is owned by this library

ISSN: 0378-8741

LA: ENGLISH

AB: An aqueous extract of Aloe vera gel was analyzed guided by modulatory activity with regard to the in vitro activation of human complement and of human polymorphnuclear leucocytes (PMN). Upon ultrafiltration a high (h-Mr) and a low (l-Mr) molecular mass fraction were obtained. Pre-incubation of human pooled serum with the h-Mr fraction resulted in a depletion of classical and alternative pathway complement activity. In contrast, only the l-Mr fraction could inhibit the production of free oxygen radicals by activated PMNs. The latter activity cannot be attributed to non-specific effects like toxicity, interference with stimulant binding or scavenger activity.

AN: 88333893

MEDLINE EXPRESS (R) 1984-1990 34 of 324

TI: Enhancement of allo-responsiveness of human lymphocytes by acemannan (Carrisyn).

AU: Womble-D; Helderman-JH

AD: Renal Immunology Laboratory, University of Texas Southwestern Medical Center, Dallas.

SO: Int-J-Immunopharmacol. 1988; 10(8): 967-74

ISSN: 0192-0561

LA: ENGLISH

AB: Healing powers have been imputed as being a feature of the gel from the aloe vera plant for centuries. The recent isolation of the active ingredient, acemannan, has made testing of this drug important. Since the drug appears to enhance monocyte function in other experiments, these studies were designed to test the capacity of acemannan to enhance immune response to alloantigen and to test whether the potential enhancement is a monocyte driven phenomenon. Acemannan did not enhance lymphocyte response to syngeneic antigens in the mixed lymphocyte culture (MLC) but importantly increased alloantigenic response in a dose-response fashion (2.6 x 10(-7) - 2.6 x 10(-9)M). This effect of acemannan was shown to be a specific response and to concur with concentrations of in vitro acemannan achievable in vivo. A separate series of mixing experiments demonstrated that acemannan incubation with monocytes permitted monocyte driven signals to enhance T-cell response to lectin. It is concluded that acemannan, the active ingredient of the aloe vera plant, is an important immunoenhancer in that it increases lymphocyte response to alloantigen. It is suggested that the mechanism involves enhancement of monocyte release of IL-I under the aegis of alloantigen. This mechanism may explain in part the recently observed capacity of acemannan to abrogate viral infections in animal and man.

AN: 89108677

MEDLINE EXPRESS (R) 1984-1990 35 of 324

TI: Acute non lymphoid leukemia treatment: an update.

AU: Mandelli-F; Petti-MC; Aloe-Spiriti-MA

SO: Haematologica. 1988 Jan-Feb; 73(1): 1-6

This title is owned by this library

ISSN: 0390-6078

LA: ENGLISH

AN: 88226191

MEDLINE EXPRESS (R) 1984-1990 36 of 324

TI: Aloe vera [published erratum appears in J Am Acad Dermatol 1988 Jul;19(1 Pt 1):82]

AU: Klein-AD; Penneys-NS

AD: Department of Dermatology, University of Miami School of Medicine, FL.

SO: J-Am-Acad-Dermatol. 1988 Apr; 18(4 Pt 1): 714-20

This title is owned by this library

ISSN: 0190-9622

LA: ENGLISH

AB: We review the scientific literature regarding the aloe vera plant and its products. Aloe vera is known to contain several pharmacologically active ingredients, including a carboxypeptidase that inactivates bradykinin in vitro, salicylates, and a substance(s) that inhibits thromboxane formation in vivo. Scientific studies exist that support an antibacterial and antifungal effect for substance(s) in aloe vera. Studies and case reports provide support for the use of aloe vera in the treatment of radiation ulcers and stasis ulcers in man and burn and frostbite injuries in animals. The evidence for a potential beneficial effect associated with the use of aloe vera is sufficient to warrant the design and implementation of well-controlled clinical trials.

AN: 88228558

MEDLINE EXPRESS (R) 1984-1990 37 of 324

TI: [Studies of aloe. II. Mechanism of cathartic effect]

AU: Ishii-Y; Tanizawa-H; Takino-Y

SO: Yakugaku-Zasshi. 1988 Sep; 108(9): 904-10

ISSN: 0031-6903

LA: JAPANESE; NON-ENGLISH

AN: 89236055

MEDLINE EXPRESS (R) 1984-1990 38 of 324

TI: Metabolism of barbaloin by intestinal bacteria.

AU: Hattori-M; Kanda-T; Shu-YZ; Akao-T; Kobashi-K; Namba-T

SO: Chem-Pharm-Bull-Tokyo. 1988 Nov; 36(11): 4462-6

This title is owned by this library

ISSN: 0009-2363

LA: ENGLISH

AN: 89230603

MEDLINE EXPRESS (R) 1984-1990 39 of 324

TI: The influence of selected potential oncostatics of plant origin on the protein biosynthesis in vitro.

AU: Paszkiewicz-Gadek-A; Chlabicz-J; Galasinski-W

AD: Department of General and Organic Chemistry, Medical Academy, Bialystok, Poland.

SO: Pol-J-Pharmacol-Pharm. 1988 Mar-Apr; 40(2): 183-90

This title is owned by this library

ISSN: 0301-0244

LA: ENGLISH

AB: Five potential oncostatics of plant origin (reserpine, amphotericin B, rutoside, digoxin, dry aloe extract), and cyclic AMP were investigated for their effect on protein synthesis. The solutions of digoxin and dry aloe extract inhibited protein biosynthesis in vitro. The direct inhibiting effect of digoxin on the ribosomes suggests that this drug forms an inactive complex with this organelle. Therefore it can be concluded that ribosome is the target site of digoxin action. Aloin and aloeemodin are responsible for the inhibitory effect of the solution of dry aloe extract. They inhibit markedly [14C]-leucine incorporation into proteins. Aloin and aloeemodin do not influence directly the ribosomes, but they inhibit elongation factors and peptidyltransferase activities in the complete elongation system. Some preliminary experiments have shown that direct interaction between these substances and elongation factor EF-2 should be taken in account. This observation is the subject of further experiments, in which the characteristics of the inhibitory effect of the components isolated from dry aloe extract will be performed.

AN: 89184031

MEDLINE EXPRESS (R) 1984-1990 40 of 324

TI: Aloe vera. A natural approach for treating wounds, edema, and pain in diabetes.

AU: Davis-RH; Leitner-MG; Russo-JM

SO: J-Am-Podiatr-Med-Assoc. 1988 Feb; 78(2): 60-8

ISSN: 8750-7315

LA: ENGLISH

AN: 88214684

MEDLINE EXPRESS (R) 1984-1990 41 of 324

TI: Aloe vera gel hindered wound healing of experimental second-degree burns: a quantitative controlled study.

AU: Kaufman-T; Kalderon-N; Ullmann-Y; Berger-J

AD: Faculty of Medicine, Technion-Israel Institute of Technology, Israel.

SO: J-Burn-Care-Rehabil. 1988 Mar-Apr; 9(2): 156-9

ISSN: 0273-8481

LA: ENGLISH

AB: In the present study, Aloe vera gel (AVG) was applied to experimental second-degree burns in guinea pigs, and its effects on epithelialization, wound contraction, newly formed granulation tissue, and regeneration of hair follicles was compared with that effected by 1% silver sulfadiazine cream (AgSD). Epithelialization (%mean +/- SEM) on postburn day 8, 16, and 24 of the AVG-treated wounds was 38.72% +/- 2.71%, 60.34% +/- 3.28%, and 92.46% +/- 2.26%, respectively, while that of the AgSD-treated burns was 53.35% +/- 2.65%, 94.84% +/- 2.65%, and 100%, respectively (P less than .001). Contraction of the AVG-wounds was significantly higher than that of the AgSD-treated burns during 24 days of the study (P less than .001). The thickness of the newly formed granulation tissue was higher in the AVG-treated wounds (P less than .001), while the hair follicles count was significantly lower (P less than .001) compared with the AgSD-treated burns. It is concluded that this preparation of Aloe vera gel hindered the healing process of the present burn wound model when compared with 1% silver sulfadiazine cream.

AN: 88198281

MEDLINE EXPRESS (R) 1984-1990 42 of 324

TI: The effect of nerve growth factor and its antibody on mast cells in vivo.

AU: Aloe-L

AD: Istituto di Neurobiologia, C.N.R., Rome, Italy.

SO: J-Neuroimmunol. 1988 Apr; 18(1): 1-12

This title is owned by this library

ISSN: 0165-5728

LA: ENGLISH

AB: Repeated subcutaneous administration of nerve growth factor (NGF) into newborn rats caused an increase in number and size of free and tissue-adherent mast cells (MCs). In vivo and in vitro autoradiographic studies show that MCs exhibit specific binding sites for NGF. Peritoneal MCs of adult rats exposed to high titers of endogenous (autoimmunization) and exogenous NGF antibodies showed severe cytological alterations and decreased in number. Light and electron microscopic studies revealed that MC destruction took place slowly (7-10 days) and required continual exposure to high titers of NGF antibodies. The role played by NGF in the hematopoietic cell lineage is at present under study in our laboratory.

AN: 88154057

MEDLINE EXPRESS (R) 1984-1990 43 of 324

TI: Comparative evaluation of aloe vera in the management of burn wounds in guinea pigs.

AU: Rodriguez-Bigas-M; Cruz-NI; Suarez-A

AD: Surgical Research Laboratories, University of Puerto Rico School of Medicine, San Juan.

SO: Plast-Reconstr-Surg. 1988 Mar; 81(3): 386-9

This title is owned by this library

ISSN: 0032-1052

LA: ENGLISH

AB: An experimental study was designed using Hartley guinea pigs, who received full-thickness burns covering 3 percent of their body surface area by direct contact with a hot plate. A total of 40 animals were equally divided among four modalities of closed burn wound management as follows: group I: silver sulfadiazine (Silvadine); group II: aloe vera gel extract (Carrington Dermal Wound Gel); group III: salicylic acid cream (aspirin); and group IV: plain gauze occlusive dressing only. The dressings were changed daily, and the size and appearance of each burn wound were recorded until complete healing. On the sixth postburn day, quantitative burn wound cultures were made. The average time to complete healing in the control group was 50 days, and the only significant difference was found in the aloe vera-treated animals, which healed on an average of 30 days (p less than 0.02). Wound bacterial counts were effectively decreased by silver sulfadiazine (p = 0.015) and by aloe vera extract (p = 0.015). From our data it appears that aloe gel extracts permit a faster healing of burn wounds.

AN: 88125468

MEDLINE EXPRESS (R) 1984-1990 44 of 324

TI: The role of thromboxane in experimental inadvertent intra-arterial drug injections.

AU: Zachary-LS; Smith-DJ Jr; Heggers-JP; Robson-MC; Boertman-JA; Niu-XT; Schileru-RE; Sacks-RJ

SO: J-Hand-Surg-Am. 1987 Mar; 12(2): 240-5

This title is owned by this library

ISSN: 0363-5023

LA: ENGLISH

AB: Inadvertent intra-arterial injection of drugs produces a well-defined clinical syndrome whose pathophysiology remains unclear. This study was designed to determine the role of the inflammatory mediator, thromboxane, in intra-arterial drug injections. The rabbit ear model, as described by Kinmonth and Sheppard, was used. Five of the experimental groups were treated with specific or nonspecific thromboxane blocking agents and two groups served as controls. Immunohistochemical staining of the control ears showed elevated levels of thromboxane within the first 6 hours postinjury. The specific thromboxane blocking agents, methimazole and Aloe vera, showed almost complete blockade of thromboxane production. The percentage of ear survival was significantly greater in the group treated with topical Aloe vera (p less than 0.05) and even greater survival was achieved in the combined Aloe vera/methimazole group (p less than 0.01). On the basis of these results, we have begun treatment of such injuries with specific and nonspecific thromboxane blocking agents.

AN: 87167320

MEDLINE EXPRESS (R) 1984-1990 45 of 324

TI: The detection of laxative abuse.

AU: Morton-J

SO: Ann-Clin-Biochem. 1987 Jan; 24 ( Pt 1): 107-8

This title is owned by this library

ISSN: 0004-5632

LA: ENGLISH

AN: 87155129

MEDLINE EXPRESS (R) 1984-1990 46 of 324

TI: Dermabrasion-Loo-punch-excision technique for the treatment of acne-induced osteoma cutis.

AU: Fulton-JE Jr

SO: J-Dermatol-Surg-Oncol. 1987 Jun; 13(6): 655-9

This title is owned by this library

ISSN: 0148-0812

LA: ENGLISH

AB: Three patients with chronic osteoma cutis secondary to acne vulgaris were treated with the dermabrasion-Loo-punch-excision technique. Under regional nerve block with lidocaine-bipuvacaine (50:50) a uniform dermabrasion was performed across the entire face, including the hairline and 1 cm below the jawline. This exposed the foci of osteoma cutis. Then the appropriate sized Loo punch (usually the 2.0- or the 2.5-mm punch) was used to excise the bluish-gray miliary lesions. The majority of the foci were removed in one operation. Following excision, the lesions were closed with 7-0 prolene suture. To prevent crust formation postoperatively, Aloe-vera-soaked polyethylene oxide gel dressings (Vigilon) were changed twice daily following an ice water compress. Sutures were removed rapidly in 5 to 7 days to prevent the appearance of suture lines. Although one patient required an additional procedure, the cosmetic results were excellent. Only a few small residual blue "dot" lesions remained in these three cases.

AN: 87223433

MEDLINE EXPRESS (R) 1984-1990 47 of 324

TI: Intraocular herpes simplex virus injection in neonatal rats induces sympathetic nerve cell destruction: effect of nerve growth factor.

AU: Aloe-L

AD: Institute of Neurobiology, C.N.R., Rome, Italy.

SO: Int-J-Dev-Neurosci. 1987; 5(4): 357-66

This title is owned by this library

ISSN: 0736-5748

LA: ENGLISH

AB: The effect of herpes simplex virus (HSV) injection on the sympathetic nerve system of newborn rats was studied at structural, ultrastructural, and immunohistochemical levels. It was found that HSV injected into the anterior eye chamber is retrogradely transported and reaches the nerve cell bodies of the ipsilateral superior cervical ganglion (SCG) after 18-24 hr, causing complete cell destruction within 3-4 days. In subsequent days, nerve cells of the contralateral SCG, spinal sensory ganglia, chromaffin cells and brain cells also become infected and are eventually killed by the virus. Pretreatment with nerve growth factor (NGF) produces an initial protection from viral cell destruction, but does not block the final, lethal effect of the virus. These investigations demonstrate that sympathetic nerve cell destruction can be induced in newborn rodents by HSV, and that NGF treatment renders the cells, for a time-limited period, more resistant to the virus.

AN: 89022170

MEDLINE EXPRESS (R) 1984-1990 48 of 324

TI: Aloe vera and wound healing.

AU: Davis-RH; Kabbani-JM; Maro-NP

SO: J-Am-Podiatr-Med-Assoc. 1987 Apr; 77(4): 165-9

ISSN: 8750-7315

LA: ENGLISH

AN: 87225713

MEDLINE EXPRESS (R) 1984-1990 49 of 324

TI: Effect of amino acids in Aloe extract on phagocytosis by peripheral neutrophil in adult bronchial asthma.

AU: Yagi-A; Shida-T; Nishimura-H

SO: Arerugi. 1987 Dec; 36(12): 1094-101

ISSN: 0021-4884

LA: ENGLISH

AN: 88240045

MEDLINE EXPRESS (R) 1984-1990 50 of 324

TI: [Induction of lymphoblastic transformation by a polysaccharide extract of a native Madagascar plant Aloe vahombe: ALVA]

TO: Induction de la transformation lymphoblastique par un extrait polysaccharidique d'une plante endemique malgache l'Aloe vahombe: "l'ALVA".

AU: Ralamboranto-L; Rakotovao-LH; Coulanges-P; Corby-G; Janot-C; Le-Deaut-JY

AD: Universite de Madagascar, E.E.S. Sciences Antananarivo.

SO: Arch-Inst-Pasteur-Madagascar. 1987; 53(1): 227-31

This title is owned by this library

ISSN: 0020-2495

LA: FRENCH; NON-ENGLISH

AN: 88239878

MEDLINE EXPRESS (R) 1984-1990 51 of 324

TI: Topical anti-inflammatory activity of Aloe vera as measured by ear swelling.

AU: Davis-RH; Leitner-MG; Russo-JM

SO: J-Am-Podiatr-Med-Assoc. 1987 Nov; 77(11): 610-2

ISSN: 8750-7315

LA: ENGLISH

AN: 88172087

MEDLINE EXPRESS (R) 1984-1990 52 of 324

TI: CALLA-negative, TdT- and CD7-positive acute lymphoblastic leukemia: a phenotype associated with poor prognosis.

AU: De-Rossi-G; Aloe-Spiriti-MA; Cafolla-A; Gastaldi-R; Lo-Coco-F; Lopez-M; Luciani-M; Pasqualetti-D; Testi-AM; Mandelli-F

AD: Hematology Section of the Department of Human Biopathology, University La Sapienza, Rome, Italy.

SO: Diagn-Clin-Immunol. 1987; 5(3): 140-3

ISSN: 0895-0458

LA: ENGLISH

AB: Eight ALL patients displaying a CD7+, Tdt+, CD10-, T MoAbs-, myeloid MoAbs-, AP+ phenotype are described. Some patients showed well-known risk factors such as cytogenetic abnormalities, high WBC count, mediastinal mass, and/or organomegalies. The clinical behaviour was very poor and only one patient is in CR and off therapy. Therefore such a pre-T phenotype, although sometimes associated with the other risk factors, could be considered a poor prognosis phenotype.

AN: 88080877

MEDLINE EXPRESS (R) 1984-1990 53 of 324

TI: Nerve growth factor influences neurobehavioral development of newborn mice.

AU: Alleva-E; Aloe-L; Calamandrei-G

AD: Section of Neurobehavioral Pathophysiology, Laboratorio di Fisiopatologia di Organo, Roma, Italy.

SO: Neurotoxicol-Teratol. 1987 Jul-Aug; 9(4): 271-5

ISSN: 0892-0362

LA: ENGLISH

AB: Thirty-six male pups of the CD-1 outbred strain received from postnatal day 2 to day 10 a daily subcutaneous injection of either highly purified murine Nerve Growth Factor (NGF; 5 mg/kg), cytochrome c (5 mg/kg), or saline solution. NGF-treated pups showed a slight, but significant, reduction in body weight gain and a concomitant acceleration in the appearance of early signs of neurobehavioral maturation such as righting reflex, cliff aversion, and response to tactile stimulation of the perioral area. Other responses, including the ultrasonic vocalization pattern on day 7, were unaffected or affected to a lesser extent.

AN: 88065346

MEDLINE EXPRESS (R) 1984-1990 54 of 324

TI: Experimental and clinical observations on frostbite.

AU: Heggers-JP; Robson-MC; Manavalen-K; Weingarten-MD; Carethers-JM; Boertman-JA; Smith-DJ Jr; Sachs-RJ

SO: Ann-Emerg-Med. 1987 Sep; 16(9): 1056-62

ISSN: 0196-0644

LA: ENGLISH

AB: Experimental ischemia by the classic frostbite rabbit ear model clearly defined the role of thromboxane as a mediator of progressive dermal ischemia in frostbite injuries. The therapeutic groups consisted of the antiprostanoids, methylprednisolone, and aspirin combined with anti-thromboxane agents Aloe vera and methimazole, while the control group received no therapy. Survival was measured by planimetry for all groups. No tissue survival was evident in the frostbite control group. Methimazole treatment allowed 34.3% survival, Aloe vera 28.2% survival, aspirin 22.5% survival, and methylprednisolone 17.5% survival. The data compare the results of a modified frostbite protocol using ibuprofen with therapeutic modalities used by other clinical services. Of 154 patients treated for frostbite from 1982 to 1985, 56 were treated with our frostbite protocol; 98 were treated with other modalities. Of the 56 protocol patients, 18 suffered 1st degree frostbite, 25, 2nd degree frostbite, and 13, 3rd degree frostbite. For all degrees of frostbite, 67.9% healed without tissue loss, 25.0% healed with partial tissue loss, and 7% required amputation (P less than .001). Of the patients not on protocol, 11 suffered 1st degree frostbite, 51, 2nd degree frostbite, and 36, 3rd degree frostbite. Of these, 32.7% healed without tissue loss, 34.6% healed with tissue loss, and 32.7% required amputation. The morbidity of progressive dermal ischemia in frostbite may be decreased by the therapeutic use of inhibitors of the arachidonic acid cascade.

AN: 87324359

MEDLINE EXPRESS (R) 1984-1990 55 of 324

TI: Studies on the activity of individual plants of an antidiabetic plant mixture.

AU: Al-Awadi-FM; Gumaa-KA

SO: Acta-Diabetol-Lat. 1987 Jan-Mar; 24(1): 37-41

This title is owned by this library

ISSN: 0001-5563

LA: ENGLISH

AB: A blood glucose lowering extract of a mixture of five plants in use by Kuwaiti diabetics was studied for the identification of its active component(s). Only the extracts of myrrh and aloe gums effectively increased glucose tolerance in both normal and diabetic rats. The remaining components, gum olibanum, Nigella sativa seeds and gum assafoetida were without effect.

AN: 87294875

MEDLINE EXPRESS (R) 1984-1990 56 of 324

TI: Plant lectin, ATF1011, on the tumor cell surface augments tumor-specific immunity through activation of T cells specific for the lectin.

AU: Yoshimoto-R; Kondoh-N; Isawa-M; Hamuro-J

SO: Cancer-Immunol-Immunother. 1987; 25(1): 25-30

This title is owned by this library

ISSN: 0340-7004

LA: ENGLISH

AB: The possibility that a plant lectin as a carrier protein would specifically activate T cells, resulting in the augmentation of antitumor immunity was investigated. ATF1011, a nonmitogenic lectin for T cells purified from Aloe arborescens Mill, bound equally to normal and tumor cells. ATF1011 binding on the MM102 tumor cell surfaces augmented anti-trinitrophenyl (TNP) antibody production of murine splenocytes when the mice were primarily immunized with TNP-conjugated MM102 tumor cells. The alloreactive cytotoxic T cell response was also augmented by allostimulator cells binding ATF1011 on the cell surfaces. These augmented responses may be assumed to be mediated by the activation of helper T cells recognizing ATF1011 as a carrier protein. Killer T cells were induced against ATF1011 antigen in the H-2 restricted manner using syngeneic stimulator cells bearing ATF1011 on the cell surfaces. When this lectin was administered intralesionally into the tumors, induction of cytotoxic effector cells was demonstrated. These results suggest that intralesionally administered ATF1011 binds to the tumor cell membrane and activates T cells specific for this carrier lectin in situ, which results in the augmented induction of systemic antitumor immunity.

AN: 87244147

MEDLINE EXPRESS (R) 1984-1990 57 of 324

TI: Separation and quantitation of fatty acids, sterols and bile acids in feces by gas chromatography as the butyl ester-acetate derivatives.

AU: Child-P; Aloe-M; Mee-D

SO: J-Chromatogr. 1987 Mar 20; 415(1): 13-26

This title is owned by this library

ISSN: 0021-9673

LA: ENGLISH

AB: A system allowing the separation and quantitation of individual species of fecal fatty acids, sterols and bile acids in a single chromatographic step is described. The system is based on the butylation of carboxyl groups and acetylation of free hydroxyls of the compounds in fecal lipid extracts, followed by their resolution by temperature-programmed gas chromatography. As the butyl ester-acetate derivatives, fatty acids, sterols and bile acids elute separately and with no overlap on a variety of chromatographic columns, obviating the need for prior separation of each class by thin-layer or column chromatography. All common bile acids, a wide variety of sterols and keto-steroids, as well as saturated and unsaturated fatty acids may be routinely resolved. Quantitation is facilitated by the addition of the internal standards, heptadecanoic acid and nor-deoxycholic acid to each sample. With an automatic sample injector, the rapid assessment of a wide range of potential risk factors for colorectal cancer may be carried out in large numbers of samples.

AN: 87222849

MEDLINE EXPRESS (R) 1984-1990 58 of 324

TI: Lymphocutaneous sporotrichosis.

AU: Satta-S

SO: Hawaii-Med-J. 1987 Apr; 46(4): 124, 127

ISSN: 0017-8594

LA: ENGLISH

AN: 87221549

MEDLINE EXPRESS (R) 1984-1990 59 of 324

TI: Bradykinin-degrading glycoprotein in Aloe arborescens var. natalensis.

AU: Yagi-A; Harada-N; Shimomura-K; Nishioka-I

SO: Planta-Med. 1987 Feb; 53(1): 19-21

This title is owned by this library

ISSN: 0032-0943

LA: ENGLISH

AN: 87204669

MEDLINE EXPRESS (R) 1984-1990 60 of 324

TI: [Antimetastatic properties of aloe juice]

TO: Protivometastaticheskie svoistva soka aloe.

AU: Gribel'-NV; Pashinskii-VG

SO: Vopr-Onkol. 1986; 32(12): 38-40

This title is owned by this library

ISSN: 0507-3758

LA: RUSSIAN; NON-ENGLISH

AB: An evaluation of antimetastatic properties of succus Aloes was carried out using three types of experimental tumors of mice and rats. It was found that succus Aloes treatment contributes to reduction of tumor mass, metastatic foci and metastasis frequency at different stages of tumor progress without affecting major tumor growth. Succus Aloes potentiates the antitumor effect of 5-fluorouracil and cyclophosphamide as components of combination chemotherapy.

AN: 87095708

MEDLINE EXPRESS (R) 1984-1990 61 of 324

TI: The antidiabetic activity of aloes: preliminary clinical and experimental observations.

AU: Ghannam-N; Kingston-M; Al-Meshaal-IA; Tariq-M; Parman-NS; Woodhouse-N

SO: Horm-Res. 1986; 24(4): 288-94

This title is owned by this library

ISSN: 0301-0163

LA: ENGLISH

AB: The dried sap of the aloe plant (aloes) is one of several traditional remedies used for diabetes in the Arabian peninsula. Its ability to lower the blood glucose was studied in 5 patients with non-insulin-dependent diabetes and in Swiss albino mice made diabetic using alloxan. During the ingestion of aloes, half a teaspoonful daily for 4-14 weeks, the fasting serum glucose level fell in every patient from a mean of 273 +/- 25 (SE) to 151 +/- 23 mg/dl (p less than 0.05) with no change in body weight. In normal mice, both glibenclamide (10 mg/kg twice daily) and aloes (500 mg/kg twice daily) induced hypoglycaemia after 5 days, 71 +/- 6.2 and 91 +/- 7.6 mg/dl, respectively, versus 130 +/- 7 mg/dl in control animals (p less than 0.01); only glibenclamide was effective after 3 days. In the diabetic mice, fasting plasma glucose was significantly reduced by glibenclamide and aloes after 3 days. Thereafter only aloes was effective and by day 7 the plasma glucose was 394 +/- 22.0 versus 646 +/- 35.9 mg/dl, in the controls and 726 +/- 30.9 mg/dl in the glibenclamide treated group (p less than 0.01). We conclude that aloes contains a hypoglycaemic agent which lowers the blood glucose by as yet unknown mechanisms.

AN: 87055702

MEDLINE EXPRESS (R) 1984-1990 62 of 324

TI: The glucomannan system from Aloe vahombe (liliaceae). III. Comparative studies on the glucomannan components isolated from the leaves.

AU: Vilkas-E; Radjabi-Nassab-F

SO: Biochimie. 1986 Sep; 68(9): 1123-7

This title is owned by this library

ISSN: 0300-9084

LA: ENGLISH

AB: The polysaccharide mixture obtained by hot water extraction of Aloe vahombe leaves is composed of at least four different paritally acetylated glucomannans which differ in molecular weight, glucose to mannose ratios and acetyl contents. Furthermore, one fraction contains a small but significant amount of protein which could not be removed by gel filtration in a hydrogen-bond-breaking medium, by DEAE-Sephadex A-50 anion exchange chromatography, or by Sevag's method.

AN: 87049830

MEDLINE EXPRESS (R) 1984-1990 63 of 324

TI: Aggressive behavior induces release of nerve growth factor from mouse salivary gland into the bloodstream.

AU: Aloe-L; Alleva-E; Bohm-A; Levi-Montalcini-R

SO: Proc-Natl-Acad-Sci-U-S-A. 1986 Aug; 83(16): 6184-7

This title is owned by this library

ISSN: 0027-8424

LA: ENGLISH

AB: Intraspecific fighting induced by 6-8 weeks of social isolation results in massive release of nerve growth factor (NGF) into the bloodstream of adult male mice. The amount of circulating NGF is highly correlated with the number of fighting episodes. Biological, radioimmunological, immunohistochemical, and ultrastructural studies show that NGF is discharged from the salivary gland into the blood within minutes after fighting and reaches the highest level 3-4 hr later. Adrenergic innervation of the salivary gland or adrenalectomy does not abolish the NGF release. Corticotropic hormones do not induce NGF increase in the blood. Daily administrations of highly purified NGF (3 micrograms per g of body weight) result in a considerable increase in the volume of adrenal glands. These findings are unequivocable evidence for a physiological role of the mouse salivary glands as a major source of blood NGF.

AN: 86287420

MEDLINE EXPRESS (R) 1984-1990 64 of 324

TI: Evaluation of a polychemotherapeutic regimen including Idarubicin (4-demethoxydaunorubicin) in relapsed acute lymphocytic leukemia.

AU: Mandelli-F; Testi-AM; Aloe-Spiriti-MA; Giona-F; Meloni-G; Moleti-ML; Amadori-S; Pacciarini-MA

SO: Haematologica. 1986 Jan-Feb; 71(1): 34-8

This title is owned by this library

ISSN: 0390-6078

LA: ENGLISH

AN: 86193790

MEDLINE EXPRESS (R) 1984-1990 65 of 324

TI: [Bacterial flora in jejunal aspirates: study using culture examination, gas chromatography and H2 breath-test]

TO: La flora batterica nell'aspirato digiunale: indagini mediante esame colturale, gas-cromatografico e H2 breath-test.

AU: Menozzi-MG; Avanzini-P; Aloe-R; Cavalli-A; Chezzi-C; Corazza-GR; Strocchi-A; Rasciti-L; Gasbarrini-G

SO: Ann-Ist-Super-Sanita. 1986; 22(3): 759-62

This title is owned by this library

ISSN: 0021-2571

LA: ITALIAN; NON-ENGLISH

AN: 87154866

MEDLINE EXPRESS (R) 1984-1990 66 of 324

TI: [Effect of aloe on the resistance of the optic nerve system of the eye]

TO: Vliianie aloe na rezistentnost' zritel'no-nervnogo apparata glaza.

AU: Dumbrova-NE; Sotnikova-Ep; Gorianova-NA

SO: Oftalmol-Zh. 1986(8): 494-6

ISSN: 0030-0675

LA: RUSSIAN; NON-ENGLISH

AN: 87145550

MEDLINE EXPRESS (R) 1984-1990 67 of 324

TI: The Aloe vera phenomenon: a review of the properties and modern uses of the leaf parenchyma gel.

AU: Grindlay-D; Reynolds-T

SO: J-Ethnopharmacol. 1986 Jun; 16(2-3): 117-51

This title is owned by this library

ISSN: 0378-8741

LA: ENGLISH

AB: The mucilaginous gel from the parenchymatous cells in the leaf pulp of Aloe vera has been used since early times for a host of curative purposes. This gel should be distinguished clearly from the bitter yellow exudate originating from the bundle sheath cells, which is used for its purgative effects. Aloe vera gel has come to play a prominent role as a contemporary folk remedy, and numerous optimistic, and in some cases extravagant, claims have been made for its medicinal properties. Modern clinical use of the gel began in the 1930s, with reports of successful treatment of X-ray and radium burns, which led to further experimental studies using laboratory animals in the following decades. The reports of these experiments and the numerous favourable case histories did not give conclusive evidence, since although positive results were usually described, much of the work suffered from poor experimental design and insufficiently large test samples. In addition some conflicting or inconsistent results were obtained. With the recent resurgence of interest in Aloe vera gel, however, new experimental work has indicated the possibility of distinct physiological effects. Chemical analysis has shown the gel to contain various carbohydrate polymers, notably either glucomannans or pectic acid, along with a range of other organic and inorganic components. Although many physiological properties of the gel have been described, there is no certain correlation between these and the identified gel components.

AN: 86309500

MEDLINE EXPRESS (R) 1984-1990 68 of 324

TI: Pretreatment of young mice with nerve growth factor enhances scopolamine-induced hyperactivity.

AU: Alleva-E; Aloe-L; Laviola-G

SO: Brain-Res. 1986 Aug; 393(2): 278-81

This title is owned by this library

ISSN: 0006-8993

LA: ENGLISH

AB: Twenty-one-day-old mice show a characteristic enhancement of locomotor activity upon injection of the cholinergic (muscarinic) blocking drug scopolamine. Intracerebral (third ventricle) injection of nerve growth factor (NGF) 24 h prior to scopolamine injection significantly enhances the drug-induced hyperactivity without modifying baseline activity. Such potentiation of the scopolamine effect was not obtained in 10-week-old mice treated and tested similarly.

AN: 86297605

MEDLINE EXPRESS (R) 1984-1990 69 of 324

TI: Wound care forum: Readers' remedies for pressure sores.

AU: Cuzzell-JZ

SO: Am-J-Nurs. 1986 Aug; 86(8): 923-4

This title is owned by this library

ISSN: 0002-936X

LA: ENGLISH

AN: 86293078

MEDLINE EXPRESS (R) 1984-1990 70 of 324

TI: Aloe vera, fiction or fact.

AU: Natow-AJ

SO: Cutis. 1986 Feb; 37(2): 106, 108

This title is owned by this library

ISSN: 0011-4162

LA: ENGLISH

AN: 86163412

MEDLINE EXPRESS (R) 1984-1990 71 of 324

TI: Antiarthritic activity of anthraquinones found in aloe for podiatric medicine.

AU: Davis-RH; Agnew-PS; Shapiro-E

SO: J-Am-Podiatr-Med-Assoc. 1986 Feb; 76(2): 61-6

ISSN: 8750-7315

LA: ENGLISH

AN: 86088605

MEDLINE EXPRESS (R) 1984-1990 72 of 324

TI: Isolation of a new aloe-emodin dianthrone diglucoside from senna and its potentiating effect on the purgative activity of sennoside A in mice.

AU: Nakajima-K; Yamauchi-K; Kuwano-S

SO: J-Pharm-Pharmacol. 1985 Oct; 37(10): 703-6

This title is owned by this library

ISSN: 0022-3573

LA: ENGLISH

AB: Two aloe-emodin dianthrone diglucosides (I and II) were isolated from the leaves of Cassia angustifolia Vahl by successive column chromatography with Amberlite XAD-2, silica gel, Polyamide C-200 and Sephadex LH-20. The stereostructures of I and II were elucidated as trans and meso isomers at 10-10', respectively, from the patterns of the ultraviolet absorption spectra and circular dichroism curves. This is the first report of isolation of diglucoside I from senna. Despite the lack of purgative activity, diglucoside I exerts a potentiating effect of about 1.3 times on the purgative activity of sennoside A in mice when even 15% is included in the mixture. The difference between I and a third active glycoside based on aloe-emodin is also discussed.

AN: 86088452

MEDLINE EXPRESS (R) 1984-1990 73 of 324

TI: Prevention of atheromatous heart disease.

AU: Agarwal-OP

SO: Angiology. 1985 Aug; 36(8): 485-92

This title is owned by this library

ISSN: 0003-3197

LA: ENGLISH

AB: Five thousand patients of atheromatous heart disease, presented as angina pectoris, were studied over a period of five years. After adding the 'Husk of Isabgol' and 'aloe vera' (an indigenous plant known as ghee-guar-ka-paththa) to the diet, a marked reduction in total serum cholesterol, serum triglycerides, fasting and post prandial blood sugar level in diabetic patients, total lipids and also increase in HDL were noted. Simultaneously the clinical profile of these patients showed reduction in the frequency of anginal attacks and gradually, the drugs, like verapamil, nifedipine, beta-blockers and nitrates, were tapered. The patients, most benefitted, were diabetics (without adding any antidiabetic drug). The exact mechanism of the action of the above two substances is not known, but it appears, that probably they act by their high fibre contents. Both these substances need further evaluation. The most interesting aspect of the study was that no untoward side effect was noted and all the five thousand patients are surviving till date.

AN: 85304965

MEDLINE EXPRESS (R) 1984-1990 74 of 324

TI: Topical effect of aloe with ribonucleic acid and vitamin C on adjuvant arthritis.

AU: Davis-RH; Shapiro-E; Agnew-PS

SO: J-Am-Podiatr-Med-Assoc. 1985 May; 75(5): 229-37

ISSN: 8750-7315

LA: ENGLISH

AN: 85236976

MEDLINE EXPRESS (R) 1984-1990 75 of 324

TI: Cyclocytidine-induced release of nerve growth factor from mouse submandibular glands enhances regeneration of sympathetic fibers in adult mice.

AU: Aloe-L; Cozzari-C; Levi-Montalcini-R

SO: Brain-Res. 1985 Apr 22; 332(2): 259-65

This title is owned by this library

ISSN: 0006-8993

LA: ENGLISH

AB: The injection of a drug endowed with the property of stimulating alpha-adrenergic receptors, cyclocytidine (Cyclo-C), produces drastic depletion of NGF from the granular convoluted tubules (GCT) of the mouse submaxillary salivary gland and a marked NGF level increase in the bloodstream. The NGF discharged from the gland gains access to the blood. Histological studies, immunohistochemistry, in vitro biological assays and radioimmunoassays gave evidence for the growth response elicited by the endogenously released salivary NGF in intact and surgically axotomized sympathetic ganglia. These results suggest that the mouse salivary NGF displays a biological activity on its target sympathetic nerve cells.

AN: 85200795

MEDLINE EXPRESS (R) 1984-1990 76 of 324

TI: [Evaluation of the effectiveness of long-term dispensary observation and treatment of patients with osteoarthrosis deformans]

TO: Otsenka effektivnosti dlitel'nogo dispansernogo nabliudeniia i lecheniia bol'nykh deformiruiushchim osteoartrozom.

AU: Burkhanova-PA

SO: Ter-Arkh. 1985; 57(8): 143-5

This title is owned by this library

ISSN: 0040-3660

LA: RUSSIAN; NON-ENGLISH

AB: During 1979-1984, 240 outpatients aged 30 to 65 years suffering from primary osteoarthrosis deformans with primary involvement of the knee joints were followed up. One of the dispensary groups consisted of 180 patients with osteoarthrosis deformans without synovitis, the other one of 60 patients with associated osteoarthrosis and synovitis. Prolonged follow up and repeated multiple modality treatment inhibited the progression of the pathological process, reduced the clinical and functional disease manifestations permitting the patients' work fitness to be preserved.

AN: 86071062

MEDLINE EXPRESS (R) 1984-1990 77 of 324

TI: Comparative effectiveness of topical treatments for hydrofluoric acid burns.

AU: Bracken-WM; Cuppage-F; McLaury-RL; Kirwin-C; Klaassen-CD

SO: J-Occup-Med. 1985 Oct; 27(10): 733-9

This title is owned by this library

ISSN: 0096-1736

LA: ENGLISH

AB: Hydrofluoric acid (HF) burns are characterized by progressive tissue necrosis and severe pain. Numerous topical treatments have been proposed, yet few have been studied experimentally. The present study was designed to examine the comparative efficacy of recommended treatments. Hair on the hind legs of rats was removed and 48 hours later 70% HF was applied. Calcium gluconate, Zephiran (benzalkonium chloride), A + D Ointment, aloe gel, and magnesium ointment were applied topically and burn development was monitored. Calcium gluconate significantly reduced burn size as early as one hour after application. Significant protection continued for seven days after the single application. The other treatments were not effective in decreasing or delaying HF burn development. The results indicated that calcium gluconate ointment was the most effective topical treatment for HF burns.

AN: 86061946

MEDLINE EXPRESS (R) 1984-1990 78 of 324

TI: [Treatment of children with persistent cough in pharyngitis]

TO: Lechenie detei, stradaiushchikh dlitel'nym kashlem pri faringite.

AU: Grigor'eva-IF

SO: Vestn-Otorinolaringol. 1985 Jul-Aug(4): 13-6

This title is owned by this library

ISSN: 0042-4668

LA: RUSSIAN; NON-ENGLISH

AN: 86020430

MEDLINE EXPRESS (R) 1984-1990 79 of 324

TI: Differentiating effects of murine nerve growth factor in the peripheral and central nervous systems of Xenopus laevis tadpoles.

AU: Levi-Montalcini-R; Aloe-L

SO: Proc-Natl-Acad-Sci-U-S-A. 1985 Oct; 82(20): 7111-5

This title is owned by this library

ISSN: 0027-8424

LA: ENGLISH

AB: The present investigation was directed toward exploration of the spectrum of action of murine nerve growth factor (m-NGF) in peripheral cells and neurons in the central nervous system (CNS) of Xenopus laevis tadpoles. It was found that systemic m-NGF injections elicit growth and differentiative effects not only on sensory and sympathetic nerve cells but also on several populations in the CNS. The finding that aminergic and peptidergic neurons in brain centers are highly receptive to m-NGF activity provides evidence for the broad spectrum of action of this molecule in lower vertebrates and calls for a systematic search for these and other putative target cells in the CNS of higher vertebrates.

AN: 86016845

MEDLINE EXPRESS (R) 1984-1990 80 of 324

TI: Effect of Aloe extract on peripheral phagocytosis in adult bronchial asthma.

AU: Shida-T; Yagi-A; Nishimura-H; Nishioka-I

SO: Planta-Med. 1985 Jun(3): 273-5

This title is owned by this library

ISSN: 0032-0943

LA: ENGLISH

AN: 85298907

MEDLINE EXPRESS (R) 1984-1990 81 of 324

TI: Liquid chromatographic determination of barbaloin (aloin) in foods.

AU: Yamamoto-M; Ishikawa-M; Masui-T; Nakazawa-H; Kabasawa-Y

SO: J-Assoc-Off-Anal-Chem. 1985 May-Jun; 68(3): 493-4

This title is owned by this library

ISSN: 0004-5756

LA: ENGLISH

AB: A simple and rapid liquid chromatographic method is described for the determination of barbaloin (aloin, 10-D-glucopyranosyl-1,8-dihydroxy-3-(hydroxymethyl)-9(10H)-anthraceno ne) in foods. Barbaloin is extracted with water from foods containing aloe and the extract is cleaned up on a disposable cartridge by using methanol-water (55 + 45) as eluant. The eluted barbaloin is separated by liquid chromatography on a YMC A-302 column with methanol-water (50 + 50) mobile phase, and detected at 293 nm. Recoveries of barbaloin added to foods at the levels of 0.05 and 0.50 mg/g were 94.4-100%. Assay results for commercial food samples indicated that the present method is applicable to a variety of foods supplemented with aloe.

AN: 85260998

MEDLINE EXPRESS (R) 1984-1990 82 of 324

TI: Effect of aloe lectin on deoxyribonucleic acid synthesis in baby hamster kidney cells.

AU: Yagi-A; Machii-K; Nishimura-H; Shida-T; Nishioka-I

SO: Experientia. 1985 May 15; 41(5): 669-71

This title is owned by this library

ISSN: 0014-4754

LA: ENGLISH

AB: A homogeneous glycoprotein (mol. wt 40,000) containing 34% carbohydrate was isolated from Aloe arborescens var. natalensis. At a concentration of 5 micrograms/ml, this glycoprotein was shown to stimulate deoxyribonucleic acid (DNA) synthesis in baby hamster kidney (BHK) cells and to have the properties of a lectin which reacts with sheep blood cells. The chemical and physical properties of the glycoprotein (aloe lectin) are also discussed.

AN: 85204262

MEDLINE EXPRESS (R) 1984-1990 83 of 324

TI: Allergic contact dermatitis to compound tincture of benzoin.

AU: James-WD; White-SW; Yanklowitz-B

SO: J-Am-Acad-Dermatol. 1984 Nov; 11(5 Pt 1): 847-50

This title is owned by this library

ISSN: 0190-9622

LA: ENGLISH

AB: Nineteen cases of allergic contact dermatitis to compound tincture of benzoin are described. Patch testing to individual components revealed positive reactions to all ingredients except aloe. An alternative preparation, Mastisol, was successfully used without primarily inducing allergy. It should be considered for use before benzoin because once allergy to benzoin exists, cross-reactions to Mastisol usually occur. This is probably due to the presence of a common ingredient, styrax gum.

AN: 85080810

MEDLINE EXPRESS (R) 1984-1990 84 of 324

TI: The nerve growth factor. Established findings and controversial aspects.

AU: Calissano-P; Cattaneo-A; Biocca-S; Aloe-L; Mercanti-D; Levi-Montalcini-R

SO: Exp-Cell-Res. 1984 Sep; 154(1): 1-9

This title is owned by this library

ISSN: 0014-4827

LA: ENGLISH

AN: 84285690

MEDLINE EXPRESS (R) 1984-1990 85 of 324

TI: [Use of an ointment containing levomycetin, methyluracil and aloe extract in plastic operations with transplantation of free flaps of the mucosa or skin]

TO: Primenenie mazi, soderzhashchei levomitsetin, metiluratsil i ekstrakt aloe, pri plasticheskikh operatsiiakh s peresadkoi svobodnykh loskutov slizistoi ili kozhi.

AU: Krasnikov-PG

SO: Oftalmol-Zh. 1984(1): 14-6

ISSN: 0030-0675

LA: RUSSIAN; NON-ENGLISH

AN: 84246682

MEDLINE EXPRESS (R) 1984-1990 86 of 324

TI: [Use of immunomodulating preparations in the complex treatment of children with different forms of rheumatism]

TO: Ispol'zovanie immunomoduliruiushchikh preparatov v kompleksnom lechenii detei s razlichnymi formami revmatizma.

AU: Chalaia-AF

SO: Pediatriia. 1984 May(5): 43-5

ISSN: 0031-403X

LA: RUSSIAN; NON-ENGLISH

AN: 84272105

MEDLINE EXPRESS (R) 1984-1990 87 of 324

TI: Tic douloureux: report of successful treatment.

AU: Hayes-SM

SO: Gen-Dent. 1984 Sep-Oct; 32(5): 441-2

ISSN: 0363-6771

LA: ENGLISH

AN: 85028365

MEDLINE EXPRESS (R) 1984-1990 88 of 324

TI: Aloe Vera in dentistry.

AU: Sturm-PG; Hayes-SM

SO: J-Bergen-Cty-Dent-Soc. 1984 May; 50(8): 11-4

ISSN: 0092-9832

LA: ENGLISH

AN: 84289299

MEDLINE EXPRESS (R) 1984-1990 89 of 324

TI: Fluorophotometry of barbaloin in Aloe.

AU: Ishii-Y; Tanizawa-H; Takino-Y

SO: Chem-Pharm-Bull-Tokyo. 1984 Dec; 32(12): 4946-50

This title is owned by this library

ISSN: 0009-2363

LA: ENGLISH

AN: 85201914

MEDLINE EXPRESS (R) 1984-1990 90 of 324

TI: Further studies of the glucomannan from Aloe vahombe (liliaceae). II. Partial hydrolyses and NMR 13C studies.

AU: Radjabi-Nassab-F; Ramiliarison-C; Monneret-C; Vilkas-E

SO: Biochimie. 1984 Jul-Aug; 66(7-8): 563-7

This title is owned by this library

ISSN: 0300-9084

LA: ENGLISH

AB: The polysaccharide from Aloe vahombe (lilaceae) was submitted to partial hydrolyses by sulfuric, oxalic and phosphoric acid. Some oligosaccharides were isolated and investigated by chemical and 13C NMR spectroscopic methods. Their structure was determined. The results prove unambiguously that in the analyzed material the D-mannose is linked to the D-glucose by beta-1----4 linkages to form a carbohydrate heteropolymer.

AN: 85150286

MEDLINE EXPRESS (R) 1984-1990 91 of 324

TI: [Aloe, a succulent plant with therapeutic action]

TO: Aloe, Topfzierpflanze mit therapeutischer Wirkung.

AU: Lutomski-J

SO: Pharm-Unserer-Zeit. 1984 Nov; 13(6): 172-6

ISSN: 0048-3664

LA: GERMAN; NON-ENGLISH

AN: 85088729

MEDLINE EXPRESS (R) 1984-1990 92 of 324

TI: [Efficacy of tissue therapy in vascular pathology of the organ of vision]

TO: Effektivnost' tkanevoi terapii pri sosudistoi patologii organa zreniia.

AU: Semenova-GS; Gogina-IF; Abashina-NN

SO: Oftalmol-Zh. 1984(6): 347-50

ISSN: 0030-0675

LA: RUSSIAN; NON-ENGLISH

AN: 85062369

MEDLINE EXPRESS (R) 1984-1990 93 of 324

TI: [Therapeutic use of aloe in experimental stomach ulcers]

TO: Lechebnoe primenenie aloe pri eksperimental'nykh iazvakh zheludka.

AU: Sotnikova-EP

SO: Vrach-Delo. 1984 Jun(6): 71-4

ISSN: 0049-6804

LA: RUSSIAN; NON-ENGLISH

AN: 84302089

MEDLINE EXPRESS (R) 1984-1990 94 of 324

TI: Ethnopharmacologic study of Chinese rhubarb.

AU: Peigen-X; Liyi-H; Liwei-W

SO: J-Ethnopharmacol. 1984 May; 10(3): 275-93

This title is owned by this library

ISSN: 0378-8741

LA: ENGLISH

AB: This paper deals with an interdisciplinary study covering historic, botanical, phytochemical, pharmacological and clinical aspects of rhubarb and related species, to lay stress on the correlation between plant phylogeny, chemical constituents and purgative activity. It was found that the official rhubarbs were exclusively restricted in the Sect. Palmata, e.g. Rheum palmatum, R. palmatum var. tanguticum, R. officinale; the following criteria may serve as their standard, viz., the presence of sennoside derivatives and rhein, the occurrence of the reduced form of rhein and aloe-emodin, the leaves with any kind of palmate incision. Comprehensive multivariate analyses showed that there is a very close relationship between the leaf incision, existence of sennosides or rhein and purgative activity.

AN: 84269150

MEDLINE EXPRESS (R) 1984-1990 95 of 324

TI: Contact dermatitis from aloe arborescens.

AU: Nakamura-T; Kotajima-S

SO: Contact-Dermatitis. 1984 Jul; 11(1): 51

This title is owned by this library

ISSN: 0105-1873

LA: ENGLISH

AN: 84260543

MEDLINE EXPRESS (R) 1984-1990 96 of 324

TI: Treatment for flash burns of the conjunctiva [letter]

AU: Lawrence-D

SO: N-Engl-J-Med. 1984 Aug 9; 311(6): 413

This title is owned by this library

ISSN: 0028-4793

LA: ENGLISH

AN: 84245764

MEDLINE EXPRESS (R) 1984-1990 97 of 324

TI: Lonidamine and hyperthermia: clinical experience in melanoma. Preliminary results.

AU: Cavaliere-R; Di-Filippo-F; Varanese-A; Carlini-S; Calabro-A; Aloe-L; Piarulli-L

SO: Oncology. 1984; 41 Suppl 1: 116-20

This title is owned by this library

ISSN: 0030-2414

LA: ENGLISH

AB: Lonidamine alone or in combination with hyperthermic perfusion, with or without melphalan, was investigated in 12 patients with stage II, III, and IV malignant melanoma. The authors evaluated the most effective methods and sequence of Lonidamine administration. Preliminary results suggest that the highest effectiveness is obtained with the simultaneous administration of Lonidamine and hyperthermia.

AN: 84192423

MEDLINE EXPRESS (R) 1984-1990 98 of 324

TI: Structure determination of polysaccharides in Aloe saponaria (Hill.) Haw. (Liliaceae).

AU: Yagi-A; Hamada-K; Mihashi-K; Harada-N; Nishioka-I

SO: J-Pharm-Sci. 1984 Jan; 73(1): 62-5

This title is owned by this library

ISSN: 0022-3549

LA: ENGLISH

AB: Neutral polysaccharides that inhibit carrageenin-induced edema in rats were isolated from the nondialysate of the pulp of Aloe saponaria by gel filtration. These were shown to be a linear polymer of a 1,4-linked beta-D-mannopyranose (mol. wt. 15,000) containing 18% acetyl groups (As mannan 1), and a 1,4-linked alpha-D-mannopyranose polymer containing a single branch on the principal chain consisting of D-glucose residues linked at C-2 and C-4 (mol. wt. 66,000), with 10% acetyl groups (As mannan 2). As mannan 1 inhibited carrageenin-induced hind paw edema at 50 mg/kg ip in rats; As mannan 2 was not tested for pharmacological activity. A crude preparation of both As mannans was effective when given intraperitoneally, but was ineffective when given orally.

AN: 84114380

MEDLINE EXPRESS (R) 1984-1990 99 of 324

TI: Adrenal chromaffin cells form functional cholinergic synapses in culture.

AU: Ogawa-M; Ishikawa-T; Irimajiri-A

SO: Nature. 1984 Jan 5-11; 307(5946): 66-8

This title is owned by this library

ISSN: 0028-0836

LA: ENGLISH

AB: Adrenomedullary cells and autonomic ganglion cells originate from the neural crest. Both cell types synthesize, store and release catecholamines; however, their structural and functional properties are distinctly different. Aloe and Levi-Montalcini have shown in vivo that when the adrenal medulla is exposed to exogenous nerve growth factor (NGF) most cells differentiate into neuronal cells substantially similar to sympathetic neurones. Experiments in vitro have also shown that neonatal as well as adult adrenal chromaffin cells and their neoplastic correlate (PC12 cells) undergo neurone-like morphologic differentiation in response to NGF. From these morphological and biochemical studies alone, however, it remains uncertain whether the functional neuronal transformation is also accompanied. We report here that the adrenal chromaffin cells in culture can differentiate into neuronal cells having functional synapses which were found to be cholinergic in nature. Furthermore, the type of synaptic vesicles in the newly formed synapses was apparently dependent upon K+ levels in the culture medium.

AN: 84093594

MEDLINE EXPRESS (R) 1991 - 1995 100 of 324

TI: High-performance liquid chromatographic profiles of aloe constituents and determination of aloin in beverages, with reference to the EEC regulation for flavouring substances.

AU: Zonta-F; Bogoni-P; Masotti-P; Micali-G

AD: Istituto di Statistica e Ricerca Operativa, Universita di Trento, Italy.

SO: J-Chromatogr-A. 1995 Dec 1; 718(1): 99-106

This title is owned by this library

LA: ENGLISH

AB: Characteristic HPLC profiles of fresh and aged aloe solutions, detected at 360 and 220 nm, are presented and compared. Several aloe constituents (aloesin, aloeresin A, hydroxyaloin, aloin A and B and aloinoside A and B) were simultaneously separated and identified. The determination of aloin is described (detection limit 0.15 ppm) and discussed. In aloe-based alcoholic beverages, the aloins could not be detected, owing to their instability and degradation in solution; this is discussed in relation to the EEC Council Directive 88/388, which fixed the values of maximum allowable concentrations for aloin in food and beverages. Instead of aloin, other aloe compounds (e.g., aloeresin A or aloesin) should perhaps be used as an index of the presence of aloe in alcoholic beverages.

AN: 96129767

MEDLINE EXPRESS (R) 1991 - 1995 101 of 324

TI: Mandibular-Lingual Repositioning Device--MLRD: preliminary results of 8 patients with obstructive sleep apnea syndrome--OSAS.

AU: Castro-Barbosa-R; Aloe-F; Tavares-S; Baptista-Silva-A

AD: College of Dentistry of the Universidade de Sao Paulo, Brazil.

SO: Rev-Paul-Med. 1995 May-Jun; 113(3): 888-94

This title is owned by this library

ISSN: 0035-0362

LA: ENGLISH

AB: Dental devices have been employed in the treatment of snoring and obstructive sleep apnea syndrome (OSAS) of mild to moderate degrees. The authors disclose the preliminary results in 8 patients with clinically diagnosed obstructive sleep apnea polisomnographically confirmed and treated with a dental device (Mandibular-Lingual Repositioning Device--MLRD) developed by one of the authors (RCB). Cephalometrics was performed before usage of MLRD, Epworth Sleepiness Scale (ESS) tests were conducted before and after the MLRD and a questionnaire that subjectively qualified the Overall Subjective Improvement of the patient (OSI) was filled out. The tests were repeated 4 weeks after the application of the MLRD. The average subjective overall improvement was 73.75% and the Epworth Sleepiness Scale was 13.88 (pre-MLRD) and 6.63 (post-MLRD) representing a significant statistical variation (p = 0.05). The authors discuss probable factors involved in the improvement of the subjective measurements of excessive sleepiness.

AN: 96318392

MEDLINE EXPRESS (R) 1991 - 1995 102 of 324

TI: Monosodium glutamate increases NGF and NPY concentrations in rat hypothalamus and pituitary.

AU: Tirassa-P; Lundeberg-T; Stenfors-C; Bracci-Laudiero-L; Theodorsson-E; Aloe-L

AD: Institute of Neurobiology, Rome, Italy.

SO: Neuroreport. 1995 Dec 15; 6(18): 2450-2

ISSN: 0959-4965

LA: ENGLISH

AB: The effects of MSG treatment on NGF and NPY levels were analysed in the hypothalamus, pituitary, adrenal, thyroid and testis of adult rats. Daily i.v. injections of MSG (1 g kg-1 for 1 week) induced an increase of NGF in the hypothalamus (control (C) = 378 +/- 54; saline (S) = 369 +/- 36; MSG = 479 +/- 35 pg g-1 tissue; p < 0.001) and pituitary (C = 310 +/- 34; S = 376 +/- 114; MSG = 576 +/- 98 pg g-1 tissue; p < 0.01). Hypothalamic and pituitary NPY concentrations were also altered in the MSG-treated rats. Compared with saline-treated rats, the NPY concentration increased by 43% in the hypothalamus and 37.5% in the pituitary of MSG-treated rats. No significant changes in NGF and NPY content were found in the adrenal or thyroid of treated animals. These results suggest that hypothalamic and pituitary NGF and NPY levels may be involved in the control of neuroendocrine functions that are affected by MSG treatment.

AN: 96342954

MEDLINE EXPRESS (R) 1991 - 1995 103 of 324

TI: A 35 kDa mannose-binding lectin with hemagglutinating and mitogenic activities from "Kidachi Aloe" (Aloe arborescens Miller var. natalensis Berger).

AU: Koike-T; Beppu-H; Kuzuya-H; Maruta-K; Shimpo-K; Suzuki-M; Titani-K; Fujita-K

AD: Institute of Pharmacognosy, Fujita Health University, Mie.

SO: J-Biochem-Tokyo. 1995 Dec; 118(6): 1205-10

This title is owned by this library

ISSN: 0021-924X

LA: ENGLISH

AB: A novel lectin was isolated from the leaf skin of "Kidachi Aloe" (Aloe arborescens Miller var. natalensis Berger) by sequential chromatographies on Sephadex G-25 gel filtration, DEAE ion exchange, and Superdex 75 gel filtration columns. The native lectin exhibited a molecular mass of about 35 kDa on both gel filtration on a Superdex 75 column and native-PAGE under nonreducing conditions. SDS-PAGE in the presence or absence of beta-mercaptoethanol revealed two distinct peptides with molecular masses of about 5.5 and 2.3 kDa, respectively, in addition to a major 9.2 kDa subunit, indicating the presence of a partially processed subunit. The N-terminal amino acid sequence of the intact subunit showed homology with that of snowdrop lectin. The native lectin showed hemagglutinating activity toward rabbit but not human and sheep erythrocytes, and specifically bound to mannose like snowdrop lectin did, indicating that the Aloe and snowdrop lectins are structurally and functionally similar proteins. In addition, the native lectin showed strong mitogenic activity toward mouse lymphocytes.

AN: 96362159

MEDLINE EXPRESS (R) 1991 - 1995 104 of 324

TI: Sleep-related laryngospasm.

AU: Aloe-FS; Thorpy-MJ

AD: Department of Neurology, University of Sao Paulo Medical School, Brasil.

SO: Arq-Neuropsiquiatr. 1995 Mar; 53(1): 46-52

This title is owned by this library

ISSN: 0004-282X

LA: ENGLISH

AB: Seven patients (mean age 46.6; range 33-58; 6M,1F) presented with sleep-related choking episodes and were found to have features in common that distinguished them from other known causes of choking episodes during sleep. The characteristic features include: an awakening from sleep with an acute choking sensation, stridor, panic, tachycardia, short duration of episode (less than 60 seconds), infrequent episodes (typically less than 1 per month), and absence of any known etiology. The disorder most commonly occurs in middle-aged males who are otherwise healthy. In one patient an episode of laryngospasm was polysomnographically documented to occur during stage 3. The clinical features and the polysomnographic findings suggest spasm of the vocal cords of unknown etiology.

AN: 96035632

MEDLINE EXPRESS (R) 1991 - 1995 105 of 324

TI: Effect of aloe vera gel to healing of burn wound a clinical and histologic study.

AU: Visuthikosol-V; Chowchuen-B; Sukwanarat-Y; Sriurairatana-S; Boonpucknavig-V

AD: Department of Surgery, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.

SO: J-Med-Assoc-Thai. 1995 Aug; 78(8): 403-9

ISSN: 0125-2208

LA: ENGLISH

AB: In a study of twenty-seven patients with partial thickness burn wound, they were treated with aloe vera gel compared with vaseline gauze. It revealed the aloe vera gel treated lesion healed faster than the vaseline gauze area. The average time of healing in the aloe gel area was 11.89 days and 18.19 days for the vaseline gauze treated wound. Statistical analysis by using t-test and the value of P < 0.002 was statistically significant. In histologic study, it showed early epithelialization in the treated aloe vera gel area. Only some minor adverse effects, such as discomfort and pain were encountered in the 27 cases. This study showed the effectiveness of aloe vera gel on a partial thickness burn wound, and it might be beneficial to do further trials on burn wounds.

AN: 96033500

MEDLINE EXPRESS (R) 1991 - 1995 106 of 324

TI: Nitric oxide production by chicken macrophages activated by Acemannan, a complex carbohydrate extracted from Aloe vera.

AU: Karaca-K; Sharma-JM; Nordgren-R

AD: University of Minnesota, College of Veterinary Medicine, Department of Pathobiology, St Paul 55108, USA.

SO: Int-J-Immunopharmacol. 1995 Mar; 17(3): 183-8

ISSN: 0192-0561

LA: ENGLISH

AB: Cultures of normal chicken spleen cells and HD11 line cells produce nitric oxide (NO) in response to Acemannan, a complex carbohydrate derived from the Aloe vera plant. Neither cell type produced detectable amounts of NO in response to similar concentrations of yeast mannan, another complex carbohydrate. Nitric oxide production was dose dependent and inhibitable by the nitric oxide synthase inhibitor NG-methyl-L-arginine. In addition, the production of NO was inhibited by preincubation of ACM with concanavalin A in a dose-dependent manner. These results suggest that ACM-induced NO synthesis may be mediated through macrophage mannose receptors, and macrophage activation may be accountable for some of the immunomodulatory effects of ACM in chickens.

AN: 96053455

MEDLINE EXPRESS (R) 1991 - 1995 107 of 324

TI: Age and laxative use in hospitalized patients. A report of the "Gruppo Italiano di Farmacovigilanza nell'Anziano--GIFA".

AU: Pahor-M; Mugelli-A; Guralnik-JM; Manto-A; Carosella-L; Sgadari-A; Carbonin-PU

AD: Istituto di Medicina Interna e Geriatria, Univesita Cattolica del Sacro Cuore, Roma, Italy.

SO: Aging-Milano. 1995 Apr; 7(2): 128-35

ISSN: 0394-9532

LA: ENGLISH

AB: Laxatives are frequently taken especially by older persons, who have multiple diseases and are disabled. Major differences exist among countries in the type of laxatives taken, and knowledge of the intake patterns is important because of the potential adverse effects associated with particular ingredients. Laxatives and factors related to their use are described in a sample of 3257 patients admitted to 58 hospitals of the Gruppo Italiano di Farmacovigilanza nell'Anziano (GIFA) in 1991. The mean age was 68.2 years, median 73 years, more than 30% were age > or = 80 years, and 48.7% were men. The overall prevalence rates of laxative use during hospital stay and prior to admission were 11.5% and 11.3%, respectively. Increasing age was independently associated with laxative use after adjusting for gender, activities of daily living, cognitive function, number of active medical problems, medications taken, Italian region and length of stay. After excluding the prescriptions for hyperammoniemia, lactulose was the most frequently taken laxative (prevalence: 6.7% during hospital stay and 4.3% prior to hospital admission), followed by anthranoid laxatives such as senna, cascara, rhein and aloe (1.9% in-hospital and 3.3% pre-hospital). Other less frequently taken laxatives were picosulfate, phenolphthalein, lactitol, glycerol, bisacodyl and docusate. These patterns differ substantially from those reported by surveys conducted in other countries. Further studies are needed to assess the risks and benefits related to the intake of specific laxative ingredients.

AN: 96044906

MEDLINE EXPRESS (R) 1991 - 1995 108 of 324

TI: Effect of NGF antibodies on mast cell distribution, histamine and substance P levels in the knee joint of TNF-arthritic transgenic mice.

AU: Aloe-L; Probert-L; Kollias-G; Micera-A; Tirassa-P

AD: Institute of Neurobiology, Rome, Italy.

SO: Rheumatol-Int. 1995; 14(6): 249-52

This title is owned by this library

ISSN: 0172-8172

LA: ENGLISH

AB: We have previously shown an increase in nerve growth factor (NGF) levels and in mast cell (MC) distribution in the synovium of patients affected by rheumatoid arthritis. We now report that purified NGF antibodies injected into arthritic transgenic mice carrying the human tumour necrosis factor-alpha (TNF-alpha) gene caused reduction in the number of MCs, as well as a decrease in histamine and substance P levels within the synovium. These observations suggest that NGF antibody might be useful in studying the role of these pro-inflammatory markers in joint arthritis.

AN: 95320524

MEDLINE EXPRESS (R) 1991 - 1995 109 of 324

TI: The complete amino acid sequence of a mannose-binding lectin from "Kidachi Aloe" (Aloe arborescens Miller var. natalensis Berger).

AU: Koike-T; Titani-K; Suzuki-M; Beppu-H; Kuzuya-H; Maruta-K; Shimpo-K; Fujita-K

AD: Institute of Pharmacognosy, Fujita Health University, Mie, Japan.

SO: Biochem-Biophys-Res-Commun. 1995 Sep 5; 214(1): 163-70

This title is owned by this library

ISSN: 0006-291X

LA: ENGLISH

AB: The complete amino acid sequence of a mannose-binding lectin purified from the leaf skin of "Kidachi Aloe" (Aloe arborescens Miller var. natalensis Berger) is presented. The 109-residue sequence of the subunit was determined by analysis of peptides of the intact or S-pyridylethylated protein generated by digestion with cyanogen bromide, BNPS-skatole, Achromobacter protease I, or trypsin. The subunit contains an intrachain disulfide bridge. The sequence is highly homologous to that of a mannose-binding lectin from snowdrop bulb.

AN: 95398630

MEDLINE EXPRESS (R) 1991 - 1995 110 of 324

TI: Increased plasma levels of nerve growth factor in vernal keratoconjunctivitis and relationship to conjunctival mast cells.

AU: Lambiase-A; Bonini-S; Bonini-S; Micera-A; Magrini-L; Bracci-Laudiero-L; Aloe-L

AD: Department of Ophthalmology, University of Rome Tor Vergata, Italy.

SO: Invest-Ophthalmol-Vis-Sci. 1995 Sep; 36(10): 2127-32

This title is owned by this library

ISSN: 0146-0404

LA: ENGLISH

AB: PURPOSE. To evaluate the nerve growth factor (NGF) plasma concentration in patients with vernal keratoconjunctivitis and to correlate it with the histopathology and immunopathology of the disease. METHODS. An immunoenzymatic assay was performed to measure NGF plasma levels in patients with vernal keratoconjunctivitis and in healthy matched controls. A competitive radioimmunoassay was used to detect eosinophil cationic protein (ECP) and total specific immunoglobulin E (IgE) serum levels. Histologic evaluation was performed in tarsal and bulbar conjunctival biopsies. RESULTS. Plasma levels of NGF were significantly higher (P < 0.001) in patients with vernal keratoconjunctivitis (mean = 8224.47 +/- 7802.53 pg/ml; median = 121 pg/ml) than in controls (mean = 51.68 +/- 5.94 pg/ml; median 42.5 pg/ml). Conjunctival tissue showed a significant increase of mast cells, eosinophils, and lymphocytes in vernal keratoconjunctivitis. A significant correlation was observed between plasma levels of NGF and the number of mast cells in the tarsal conjunctiva (Cc = 0.81; P < 0.005) and bulbar conjunctiva (Cc = 0.77; P < 0.01) of patients with vernal keratoconjunctivitis. No correlation was found between NGF plasma levels and total IgE serum levels in patients with vernal keratoconjunctivitis; NGF plasma levels were inversely related to the number of circulating eosinophils (Cc = -0.61; P < 0.05) and to the increased serum levels of ECP (Cc = -0.71; P < 0.02). CONCLUSIONS. These data represent the first reported evidence of increased NGF plasma levels in an allergic human disease and suggest a possible relationship between this neurotrophic polypeptide and inflammatory cells in vernal keratoconjunctivitis.

AN: 95386412

MEDLINE EXPRESS (R) 1991 - 1995 111 of 324

TI: Changes in cerebral blood flow velocity associated with biofeedback-assisted relaxation treatment of migraine headaches are specific for the middle cerebral artery.

AU: Wauquier-A; McGrady-A; Aloe-L; Klausner-T; Collins-B

AD: Department of Neurology, Texas Tech University Health Sciences Center, Lubbock 79430, USA.

SO: Headache. 1995 Jun; 35(6): 358-62

ISSN: 0017-8748

LA: ENGLISH

AB: Twenty-five patients with diagnosed migraine headaches were randomly assigned to a biofeedback-assisted relaxation therapy group or to a group who relaxed on their own. This study confirmed that the biofeedback trained group significantly decreased pain and medication more than the self-relax group. The best responders were those with the more elevated initial cerebral blood flow values and the changes in cerebral blood flow were specific for the middle cerebral artery.

AN: 95362531

MEDLINE EXPRESS (R) 1991 - 1995 112 of 324

TI: Elevated levels of nerve growth factor in the thalamus and spinal cord of rats affected by experimental allergic encephalomyelitis.

AU: Micera-A; De-Simone-R; Aloe-L

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: Arch-Ital-Biol. 1995 Mar; 133(2): 131-42

This title is owned by this library

ISSN: 0003-9829

LA: ENGLISH

AB: In a previous study it was shown that the levels of NGF in the cerebrospinal fluid of patients affected by multiple sclerosis (MS) increase during the acute phase of the disease and decrease in the remission phase. In the present study, using an animal model of MS, we investigate whether any changes in NGF levels occur in brains of rats affected by Experimental Allergic Encephalomyelitis (EAE). The results show an enhanced level of NGF in the thalamus and spinal cord and a numerical increase of mast cells (MCs) expressing mRNA-NGF localized in these two regions. These observations suggest that NGF is involved in EAE and that MCs contribute to the local increase of NGF.

AN: 95351847

MEDLINE EXPRESS (R) 1991 - 1995 113 of 324

TI: Geographical variation in the major compounds of Aloe ferox leaf exudate.

AU: van-Wyk-BE; van-Rheede-van-Oudtshoorn-MC; Smith-GF

AD: Department of Botany, Rand Afrikaans University, Johannesburg, South Africa.

SO: Planta-Med. 1995 Jun; 61(3): 250-3

This title is owned by this library

ISSN: 0032-0943

LA: ENGLISH

AB: Geographical variation in fresh Aloe ferox leaf exudate of which the dried product is commercially known as Cape Aloes, was investigated throughout the natural distribution range of the species. The composition of the major compounds is remarkably invariable, with aloeresin A, aloesin, and aloin (both epimers A and B) contributing between 70% and 97% of total dry weight, in a ratio of approximately 4:3:2, respectively. Minor compounds are less evenly distributed, with aloinoside A and aloinoside B more frequent in the western parts of the distribution area and aloeresin C and 5-hydroxyaloin A generally present in small quantities throughout the distribution area. The aloin content of the exudate is clearly related to provenance but there are no distinct geographical discontinuities. The selection of high-yielding provenances, with total aloin levels above 25%, is recommended for commercial cultivation.

AN: 95343017

MEDLINE EXPRESS (R) 1991 - 1995 114 of 324

TI: Acemannan-containing wound dressing gel reduces radiation-induced skin reactions in C3H mice.

AU: Roberts-DB; Travis-EL

AD: Department of Experimental Radiotherapy, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.

SO: Int-J-Radiat-Oncol-Biol-Phys. 1995 Jul 15; 32(4): 1047-52

This title is owned by this library

ISSN: 0360-3016

LA: ENGLISH

AB: PURPOSE: To determine (a) whether a wound dressing gel that contains acemannan extracted from aloe leaves affects the severity of radiation-induced acute skin reactions in C3H mice; (b) if so, whether other commercially available gels such as a personal lubricating jelly and a healing ointment have similar effects; and (c) when the wound dressing gel should be applied for maximum effect. METHODS AND MATERIALS: Male C3H mice received graded single doses of gamma radiation ranging from 30 to 47.5 Gy to the right leg. In most experiments, the gel was applied daily beginning immediately after irradiation. To determine timing of application for best effect, gel was applied beginning on day -7, 0, or +7 relative to the day of irradiation (day 0) and continuing for 1, 2, 3, 4, or 5 weeks. The right inner thigh of each mouse was scored on a scale of 0 to 3.5 for severity of radiation reaction from the seventh to the 35th day after irradiation. Dose-response curves were obtained by plotting the percentage of mice that reached or exceeded a given peak skin reaction as a function of dose. Curves were fitted by logit analysis and ED50 values, and 95% confidence limits were obtained. RESULTS: The average peak skin reactions of the wound dressing gel-treated mice were lower than those of the untreated mice at all radiation doses tested. The ED50 values for skin reactions of 2.0-2.75 were approximately 7 Gy higher in the wound dressing gel-treated mice. The average peak skin reactions and the ED50 values for mice treated with personal lubricating jelly or healing ointment were similar to irradiated control values. Reduction in the percentage of mice with skin reactions of 2.5 or more was greatest in the groups that received wound dressing gel for at least 2 weeks beginning immediately after irradiation. There was no effect if gel was applied only before irradiation or beginning 1 week after irradiation. CONCLUSION: Wound dressing gel, but not personal lubricating jelly or healing ointment, reduces acute radiation-induced skin reactions in C3H mice if applied daily for at least 2 weeks beginning immediately after irradiation.

AN: 95332055

MEDLINE EXPRESS (R) 1991 - 1995 115 of 324

TI: Treatment of experimental frostbite with pentoxifylline and aloe vera cream.

AU: Miller-MB; Koltai-PJ

AD: Division of Otolaryngology, Albany (NY) Medical College, USA.

SO: Arch-Otolaryngol-Head-Neck-Surg. 1995 Jun; 121(6): 678-80

This title is owned by this library

ISSN: 0886-4470

LA: ENGLISH

AB: OBJECTIVE: To compare the therapeutic effects of systemic pentoxifylline and topical aloe vera cream in the treatment of frostbite. DESIGN: The frostbitten ears of 10 New Zealand white rabbits were assigned to one of four treatment groups: untreated controls, those treated with aloe vera cream, those treated with pentoxifylline, and those treated with aloe vera cream and pentoxifylline. MAIN OUTCOME MEASURES: Tissue survival was calculated as the percent of total frostbite area that remained after 2 weeks. RESULTS: The control group had a 6% tissue survival. Tissue survival was notably improved with pentoxifylline (20%), better with aloe vera cream (24%), and the best with the combination therapy (30%). CONCLUSION: Pentoxifylline is as effective as aloe vera cream in improving tissue survival after frostbite injury.

AN: 95290168

MEDLINE EXPRESS (R) 1991 - 1995 116 of 324

TI: Bioactive anthraquinone glycosides from Picramnia antidesma spp. fessonia.

AU: Solis-PN; Ravelo-AG; Gonzalez-AG; Gupta-MP; Phillipson-JD

AD: Department of Pharamacognosy, School of Pharmacy, London, U.K.

SO: Phytochemistry. 1995 Jan; 38(2): 477-80

This title is owned by this library

ISSN: 0031-9422

LA: ENGLISH

AB: A bioactivity guided fractionation, using KB cells and brine shrimp assays, of the methanolic extract from the leaves of Picramnia antidesma yielded two known anthraquinones, aloe-emodin and aloe-emodin anthrone, and three new aloe-emodin C-glycosides, named picramnioside A, picramnioside B and picramnioside C. Structures were established by spectroscopic methods (UV, IR, mass spectrometry, 1H and 13C and 2D NMR including COSY 45, HMQC, HMBC and ROESY). CD was used to establish the absolute configuration of the picramniosides.

AN: 95290152

MEDLINE EXPRESS (R) 1991 - 1995 117 of 324

TI: [Studies on chemical radioprotectors against X-irradiation used by soft X-ray accelerator]

AU: Shinoda-M

AD: Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan.

SO: Yakugaku-Zasshi. 1995 Jan; 115(1): 24-41

ISSN: 0031-6903

LA: JAPANESE; NON-ENGLISH

AB: This review describes the modes of mice radiation injuries induced by soft X-irradiation under various conditions and the protective effects of several kinds of substances on these injuries. The models of radiation injuries in this study were bone marrow death after lethal irradiation, skin damage induced by irradiation with long length soft X-ray and leukocytopenia in the peripheral blood after sublethal irradiation. Two bioassay methods were established for the survival effect on the lethal irradiation and protective potency on the skin damage induced by soft X-irradiation. The protective potencies of various sulfur compounds, related compounds of ferulic acid, nucleic acid constitutional compounds, crude drugs and chinese traditional medicines were determined and then many effective drugs were recognized. Effective components in the methanol extracts of Cnidii Rhizoma and Aloe arborescens recognized as radioprotectable were fractionated. As a result of these studies, it was observed that the active principles in Cnidii Rhizoma were identified as ferulic acid and adenosine. The scavenge action of active oxygens, a protective effect on the damages of deoxyribonucleic acid and superoxide dismutase by in vitro soft X-irradiation were evaluated as radiation protective mechanisms.

AN: 95213939

MEDLINE EXPRESS (R) 1991 - 1995 118 of 324

TI: Early exposure to aluminium affects eight-arm maze performance and hippocampal nerve growth factor levels in adult mice.

AU: Santucci-D; Rankin-J; Laviola-G; Aloe-L; Alleva-E

AD: Section of Behavioural Pathophysiology, Istituto Superiore di Sanita, Roma, Italy.

SO: Neurosci-Lett. 1994 Jan 17; 166(1): 89-92

This title is owned by this library

ISSN: 0304-3940

LA: ENGLISH

AB: Aluminium has been implicated in the etiology of several human pathologies but, to date, little attention has been given to the potential damage caused by gestational exposure to aluminium or to any long-term effect of early exposure. Aluminium sulphate (200 mg/kg) was administered intraperitoneally to pregnant female mice (days 10 to 13 of gestation), and male progeny was assessed in a radial eight-arm maze task at 70 days (20 daily sessions, 50 microliters water as reward). Overall, aluminium-exposed mice performed less efficiently in the radial maze than the vehicle-exposed animals. Neurobiological data showed that aluminium mice also had a higher level of nerve growth factor in the hippocampal formation when compared to controls.

AN: 94247749

MEDLINE EXPRESS (R) 1991 - 1995 119 of 324

TI: Cold water swimming stress alters NGF and low-affinity NGF receptor distribution in developing rat brain.

AU: Aloe-L; Tirassa-P; Alleva-E

AD: Istituto di Neurobiologia, CNR, Rome, Italy.

SO: Brain-Res-Bull. 1994; 33(2): 173-8

This title is owned by this library

ISSN: 0361-9230

LA: ENGLISH

AB: We have previously shown that the nerve growth factor (NGF) is released into the bloodstream following intraspecific fighting behaviour and that the level released correlates with the number of fighting episodes. We subsequently reported that NGF and its messenger RNA are present in identified hypothalamic nuclei and increase following intermale fighting behaviour. This report provides data showing that in 16-day-old rats cold water swimming stress (CWSS) alters the distribution of low-affinity NGF-Receptors (p75NGFR) and NGF levels in the central nervous system. A significant increase of NGF level was observed in the cortex, while the p75NGFR immunoreactivity decreased in neurons of the septum, nucleus basalis and striatum. Choline acetyltransferase activity in forebrain tissues remained at baseline levels. Our result suggests that NGF and p75NGFR, involved in the development and differentiation of the nervous system, are affected by stress.

AN: 94101435

MEDLINE EXPRESS (R) 1991 - 1995 120 of 324

TI: Neurological complications of coronary arteriography.

AU: Mutarelli-EG; Arie-S; Aloe-F; Scaff-M

AD: Neurological Clinic Divison, General Clinical Hospital, Medical Faculty of Sao Paulo, USP.

SO: Rev-Paul-Med. 1994 Oct-Dec; 112(4): 646-8

This title is owned by this library

ISSN: 0035-0362

LA: ENGLISH

AB: The authors report a 46 year-old female who was submitted to a right femoral artery coronary arteriography. During the procedure she presented an upward, downward and convergence gaze paresis; an upbeating nystagmus. A NMRI showed a change in T-2 weighted signal of the left paramedian mesencephalic region. A diagnosis of stroke was made and dextran 40 was prescribed. Vertical gaze paresis progressively disappeared. Convergence paresis and nystagmus improved partially only. The possible pathophysiological basis underlying this clinical picture are: catheter-induced dislodgement of an atheromatous plaque; small thrombi formation and release from the catheter tip; catheter-induced vascular lesion with subsequent thromboembolism or dissection of the intima; cardiac arrhythmia; catheter-induced or contrast medium-induced arterial spasm; a combination of two or more of these.

AN: 96090533

MEDLINE EXPRESS (R) 1991 - 1995 121 of 324

TI: Nocturnal eating syndrome: a case report with therapeutic response to dexfenfluramine.

AU: Mancini-MC; Aloe-F

AD: Department of Endocrinology, University of Sao Paulo Medical School, General Hospital.

SO: Rev-Paul-Med. 1994 Apr-Jun; 112(2): 569-71

This title is owned by this library

ISSN: 0035-0362

LA: ENGLISH

AB: A woman with nocturnal eating syndrome responsive to dexfenfluramine (DXF) is reported. Eating consisted of nightly ingestion of large amounts of high-calorie meals and often sloppy meal consumption or preparation. Amnesia for the episodes was total. Anorexigenic medications produced partial control of her daytime carbohydrate craving and no nocturnal eating change. DXF stopped her eating behavior completely. Nocturnal eating herein meets all 4 DSM-III-R diagnostic criteria for binge eating disorder. 5-HT role in neural process controlling sleep-wakefulness (SW) has been widely shown. A 5-HT agonist like DXF could determine changes in the SW processes producing the therapeutic outcome reported herein. However, a specific DXF effect on the behavioral control of carbohydrate ingestion can not be dismissed.

AN: 95334663

MEDLINE EXPRESS (R) 1991 - 1995 122 of 324

TI: Nerve growth factor and autoimmune diseases.

AU: Aloe-L; Skaper-SD; Leon-A; Levi-Montalcini-R

AD: Institute of Neurobiology, Consiglio Nazionale delle Ricerche, Rome, Italy.

SO: Autoimmunity. 1994; 19(2): 141-50

This title is owned by this library

ISSN: 0891-6934

LA: ENGLISH

AB: The initiation of a humoral immune response to a foreign antigen is a complex biologic process involving the interaction of many cell types and their secreted products. Autoimmune diseases, which are characterized by an abnormal activation of the immune system, probably result from the failure of normal self-tolerance mechanisms. The etiology of such illnesses, however, is far from being understood. While there have been extensive studies on the participation of the immune and endocrine systems in autoimmune diseases, few have dealt with nervous system-mediated immunoregulation in such situations. Evidence continues to grow suggesting that nerve growth factor (NGF), first identified for its activity in promoting the growth and differentiation of sensory and sympathetic neurons, may exert a modulatory role on neuroimmunoendocrine functions of vital importance in the regulation of homeostatic processes. Newly detected NGF-responsive cells belong to the hemopoietic-immune system and to populations in the brain involved in neuroendocrine functions. NGF levels are elevated in a number of autoimmune states, along with increased accumulation of mast cells. NGF and mast cells both appear to be involved in neuroimmune interactions and tissue inflammation. Moreover, mast cells themselves synthesize, store, and release NGF, proposing that alterations in normal mast cell behaviors may provoke maladaptive neuroimmune tissue responses whose consequences could have profound implications in inflammatory disease states, including those of an autoimmune nature. This review focuses on these cellular events and presents a working model which attempts to explain the close interrelationships of the neuroendocrinoimmune triade via a modulatory action of NGF.

AN: 95290609

MEDLINE EXPRESS (R) 1991 - 1995 123 of 324

TI: NGF effects on hot plate behaviors in mice.

AU: Della-Seta-D; de-Acetis-L; Aloe-L; Alleva-E

AD: Section of Behavioral Pathophysiology, Istituto Superiore di Sanita, Rome, Italy.

SO: Pharmacol-Biochem-Behav. 1994 Nov; 49(3): 701-5

This title is owned by this library

ISSN: 0091-3057

LA: ENGLISH

AB: Adult CD-1 male mice were injected intravenously with 2.5 micrograms/g of highly purified murine NGF and then assessed for hot plate responding (52 degrees C) at 15, 30, 60, 180, and 360 min (repeated test) or at 30, 60, or 360 min (single test, i.e., exposure to hot plate only once). Control animals received cytochrome c (2.5 micrograms/g). In the repeated test, NGF produced hyperalgesia, increasing the number of jumps (particularly at 30 and 60 min postinjection), while in the single test the pain reaction of NGF-treated animals remained unaffected. Sensitization of C-fibers in the periphery or release of bioactive mediators from mast cells may account for NGF-induced changes in nociception.

AN: 95166869

MEDLINE EXPRESS (R) 1991 - 1995 124 of 324

TI: Aloe vera, hydrocortisone, and sterol influence on wound tensile strength and anti-inflammation.

AU: Davis-RH; DiDonato-JJ; Johnson-RW; Stewart-CB

AD: Pennsylvania College of Podiatric Medicine, Philadelphia.

SO: J-Am-Podiatr-Med-Assoc. 1994 Dec; 84(12): 614-21

ISSN: 8750-7315

LA: ENGLISH

AB: Aloe vera at doses of 100 and 300 mg/kg daily for 4 days blocked the wound healing suppression of hydrocortisone acetate up to 100% using the wound tensile strength assay. This response was because of the growth factors present in A. vera masking the wound healing inhibitors such as sterols and certain amino acids. The sterols showed good anti-inflammatory activity (-36%) in reducing the croton oil-induced ear swelling. This activity displayed a dose-response relationship.

AN: 95156229

MEDLINE EXPRESS (R) 1991 - 1995 125 of 324

TI: Oral ulcers remedy gets FDA clearance [news]

SO: J-Am-Dent-Assoc. 1994 Oct; 125(10): 1308, 1310

This title is owned by this library

ISSN: 0002-8177

LA: ENGLISH

AN: 95146704

MEDLINE EXPRESS (R) 1991 - 1995 126 of 324

TI: Yawning.

AU: Aloe-F

AD: Department of Neurology, University of Sao Paulo Medical School, Brasil.

SO: Arq-Neuropsiquiatr. 1994 Jun; 52(2): 273-6

This title is owned by this library

ISSN: 0004-282X

LA: ENGLISH

AB: The ubiquitousness of normal yawning and the existence of abnormal yawning warrant an understanding of this reflex. Its mechanisms and functional role are not entirely known. A review of the literature reveals that yawning is a brain stem arousal reflex with both peripheral and central loops subserving reversal of brain hypoxia or hypoxemia. Behaviorally, yawning is a semi-involuntary act that occurs also because of loss of interest in the surroundings and it is not necessarily associated with fatigue. Socio-environmental factors can influence the emergence of yawning. Dopaminergic, acetylcholinergic, ACTHergic and oxytocinergic systems are involved in the generation and modulation of yawning in animal experimentation.

AN: 95126833

MEDLINE EXPRESS (R) 1991 - 1995 127 of 324

TI: Emotional stress induced by parachute jumping enhances blood nerve growth factor levels and the distribution of nerve growth factor receptors in lymphocytes.

AU: Aloe-L; Bracci-Laudiero-L; Alleva-E; Lambiase-A; Micera-A; Tirassa-P

AD: Institute of Neurobiology, Consiglio Nazionale delle Ricerche, Rome, Italy.

SO: Proc-Natl-Acad-Sci-U-S-A. 1994 Oct 25; 91(22): 10440-4

This title is owned by this library

ISSN: 0027-8424

LA: ENGLISH

AB: We examined the plasma nerve growth factor (NGF) level and the distribution of NGF receptors in peripheral lymphocytes of young soldiers (mean age, 20-24 yr) experiencing the thrill of a novice about to make their first parachute jumps. Blood was collected from soldiers who knew they were selected to jump (n = 26), as well as from soldiers who knew they were not selected (n = 17, controls). The former group was sampled the evening before the jump and 20 min after landing. Compared with controls, NGF levels increased 84% in prejump and 107% in postjump sampling. Our studies also showed that the increase of NGF levels preceded the increase of plasma cortisol and adrenocorticotropic hormone. No changes in the baseline levels of circulating interleukin 1 beta or tumor necrosis factor were found, suggesting that the increased levels of NGF were not correlated with change in these cytokines. Moreover, immunofluorescence analysis demonstrated that parachuting stress enhances the distribution of low-affinity p75LNGFR and high-affinity p140trkA NGF receptors in circulating peripheral blood mononuclear cells. These observations suggest that the release of NGF might be involved in the activation of cells of the immune system and is most probably associated with homeostatic adaptive mechanisms, as previously shown for stressed rodents.

AN: 95024130

MEDLINE EXPRESS (R) 1991 - 1995 128 of 324

TI: Studies of aloe. V. Mechanism of cathartic effect. (4).

AU: Ishii-Y; Tanizawa-H; Takino-Y

AD: School of Pharmaceutical Sciences, University of Shizuoka, Japan.

SO: Biol-Pharm-Bull. 1994 May; 17(5): 651-3

ISSN: 0918-6158

LA: ENGLISH

AB: Aloe-emodin-9-anthrone(AE-anthrone), produced from barbaloin in the rat large intestine, caused not only an increase in the intestinal water content but also stimulated mucus secretion. This might play an important role in the occurrence of diarrhea. It was demonstrated that the amount of AE-anthrone produced in the rat large intestine(maximal amount: 568 micrograms/rat at 4 h after injection) was enough to cause both of these effects, which were observed following intracecal administration of barbaloin (31.1 mg/kg). These results together with our previous data, which showed a relationship between increase in the intestinal water content and the stimulation of peristalsis, confirm that AE-anthrone is the principal agent responsible for the cathartic effect of barbaloin. We also propose that the increase in water content is a more important factor than stimulation of peristalsis in the induction of diarrhea by barbaloin.

AN: 95004237

MEDLINE EXPRESS (R) 1991 - 1995 129 of 324

TI: Prevention of ultraviolet radiation-induced suppression of contact and delayed hypersensitivity by Aloe barbadensis gel extract.

AU: Strickland-FM; Pelley-RP; Kripke-ML

AD: Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston 77030.

SO: J-Invest-Dermatol. 1994 Feb; 102(2): 197-204

This title is owned by this library

ISSN: 0022-202X

LA: ENGLISH

AB: We investigated the ability of Aloe barbadensis gel extract to prevent suppression of contact hypersensitivity (CHS) and delayed-type hypersensitivity (DTH) responses in mice by ultraviolet (UV) irradiation. Local immune suppression was induced in C3H mice by exposure to four daily doses of 400 J/m2 UV-B (280-320 nm) radiation from FS40 sunlamps, followed by sensitization with 0.5% fluorescein isothiocyanate (FITC) through the irradiated skin. Topical application of 0.167-1.67% Aloe gel after each irradiation significantly reduced this suppression. Aloe treatment partially preserved the number and morphology of Langerhans and Thy-1+ dendritic epidermal cells in skin, compared to those in the skin of mice given only UVR or UVR plus the vehicle. Experiments using a single (2 kJ/m2) dose of UVR followed by Aloe treatment showed that the effect of Aloe was not due to screening of the UVR. Systemic suppression of DTH to Candida albicans or CHS to FITC was induced in C3H mice exposed to 5 or 10 kJ/m2 UV-B radiation, respectively, on shaved dorsal skin and sensitized 3 d later with a subcutaneous injection of formalin-fixed Candida or FITC painted on unirradiated, ventral skin. Treatment of the UV-irradiated skin with Aloe immediately after irradiation prevented suppression of both DTH to Candida and CHS to FITC. Aloe treatment did not prevent the formation of cyclobutyl pyrimidine dimers in the DNA of UV-irradiated skin or accelerate the repair of these lesions. These studies demonstrate that topical application of Aloe barbadensis gel extract to the skin of UV-irradiated mice ameliorates UV-induced immune suppression by a mechanism that does not involve DNA damage or repair.

AN: 94149291

MEDLINE EXPRESS (R) 1991 - 1995 130 of 324

TI: Schistosoma mansoni infection enhances the levels of NGF in the liver and hypothalamus of mice.

AU: Aloe-L; Moroni-R; Mollinari-C; Tirassa-P

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: Neuroreport. 1994 May 9; 5(9): 1030-2

ISSN: 0959-4965

LA: ENGLISH

AB: Schistosoma mansoni infection in adult mice is known to cause granulomas in the liver and intestine. Using a specific enzyme-linked immunoassay, it was found that Schistosoma mansoni infection enhances the level of nerve growth factor in the liver and surprisingly also in the hypothalamus. Exogenous administration of purified NGF antibodies inhibits NGF biological activity both in the hypothalamus and liver and drastically reduces the number of NGF-responsive cells, the mast cells, present in liver granuloma. These findings and those reported by others showing the effect of NGF on cells of the immune system support the hypothesis that this molecule plays a role in neuroendocrine-immune interactions.

AN: 94362178

MEDLINE EXPRESS (R) 1991 - 1995 131 of 324

TI: Studies of aloe. IV. Mechanism of cathartic effect. (3).

AU: Ishii-Y; Tanizawa-H; Takino-Y

AD: School of Pharmaceutical Sciences, University of Shizuoka, Japan.

SO: Biol-Pharm-Bull. 1994 Apr; 17(4): 495-7

ISSN: 0918-6158

LA: ENGLISH

AB: Charcoal transport, as an indicator of the degree of peristalsis, and water content in the large intestine after the intracaecal administration of barbaloin, were measured simultaneously in the same rat. Charcoal transport was significantly accelerated at both 3.5 and 6.5 h after the administration of barbaloin. At 6.5 h, diarrhea instead of normal faeces was observed. Moreover, at 1 h before the acceleration of charcoal transport, a marked increase in the relative water content of the large intestine was observed. It appears that the increase in water content of the large intestine induced by barbaloin precedes the stimulation of peristalsis, attended by diarrhea. Therefore, it is suggested that the increase in water content is a more important factor than the stimulation of peristalsis in the diarrhea induced by barbaloin.

AN: 94348457

MEDLINE EXPRESS (R) 1991 - 1995 132 of 324

TI: Expression and function of nerve growth factor and nerve growth factor receptor on cultured keratinocytes.

AU: Pincelli-C; Sevignani-C; Manfredini-R; Grande-A; Fantini-F; Bracci-Laudiero-L; Aloe-L; Ferrari-S; Cossarizza-A; Giannetti-A

AD: Department of Hematology, University of Modena, Italy.

SO: J-Invest-Dermatol. 1994 Jul; 103(1): 13-8

This title is owned by this library

ISSN: 0022-202X

LA: ENGLISH

AB: Keratinocytes, a key cellular component both for homeostasis and pathophysiologic processes of the skin, secrete a number of cytokines and are stimulated by several growth factors. Nerve growth factor (NGF) is synthesized in the skin and basal keratinocytes express the low-affinity nerve growth factor receptor (NGF-R). We present evidence that normal human keratinocytes in culture express the low- and the high-affinity NGF-R both at the mRNA level, as determined by reverse-transcription polymerase chain reaction and at the protein level, as shown by cytofluorimetric analysis. NGF significantly stimulates the proliferation of normal human keratinocytes in culture in a dose-dependent manner. This effect can be prevented by the addition of both an anti-NGF neutralizing antibody and a high-affinity NGF-R (trk) specific inhibitor, the natural alkaloid K252a. By contrast, keratinocyte proliferation is not inhibited by an anti-low-affinity NGF-R monoclonal antibody, thus suggesting that NGF effect on human keratinocytes is mediated by the high-affinity NGF-R. Moreover, NGF mRNA is expressed in normal human keratinocytes and NGF is secreted by keratinocytes in increasing amounts during growth, as detected by enzyme-linked immunosorbent assay. These results suggest that NGF could act as a cytokine in human skin and take part in disorders of keratinocyte proliferation.

AN: 94300118

MEDLINE EXPRESS (R) 1991 - 1995 133 of 324

TI: Mast cells synthesize, store, and release nerve growth factor.

AU: Leon-A; Buriani-A; Dal-Toso-R; Fabris-M; Romanello-S; Aloe-L; Levi-Montalcini-R

AD: Researchlife, Ospedale Civile, Castelfranco Veneto, Italy.

SO: Proc-Natl-Acad-Sci-U-S-A. 1994 Apr 26; 91(9): 3739-43

This title is owned by this library

ISSN: 0027-8424

LA: ENGLISH

AB: Mast cells and nerve growth factor (NGF) have both been reported to be involved in neuroimmune interactions and tissue inflammation. In many peripheral tissues, mast cells interact with the innervating fibers. Changes in the behaviors of both of these elements occur after tissue injury/inflammation. As such conditions are typically associated with rapid mast cell activation and NGF accumulation in inflammatory exudates, we hypothesized that mast cells may be capable of producing NGF. Here we report that (i) NGF mRNA is expressed in adult rat peritoneal mast cells; (ii) anti-NGF antibodies clearly stain vesicular compartments of purified mast cells and mast cells in histological sections of adult rodent mesenchymal tissues; and (iii) medium conditioned by peritoneal mast cells contains biologically active NGF. Mast cells thus represent a newly recognized source of NGF. The known actions of NGF on peripheral nerve fibers and immune cells suggest that mast cell-derived NGF may control adaptive/reactive responses of the nervous and immune systems toward noxious tissue perturbations. Conversely, alterations in normal mast cell behaviors may provoke maladaptive neuroimmune tissue responses whose consequences could have profound implications in inflammatory disease states, including those of an autoimmune nature.

AN: 94224817

MEDLINE EXPRESS (R) 1991 - 1995 134 of 324

TI: Anti-inflammatory and wound healing activity of a growth substance in Aloe vera.

AU: Davis-RH; Donato-JJ; Hartman-GM; Haas-RC

AD: Department of Biomedical Sciences, Pennsylvania College of Podiatric Medicine, Philadelphia.

SO: J-Am-Podiatr-Med-Assoc. 1994 Feb; 84(2): 77-81

ISSN: 8750-7315

LA: ENGLISH

AB: Aloe vera improves wound healing and inhibits inflammation. Since mannose-6-phosphate is the major sugar in the Aloe gel, the authors examined the possibility of its being an active growth substance. Mice receiving 300 mg/kg of mannose-6-phosphate had improved wound healing over saline controls. This dose also had anti-inflammatory activity. The function of mannose-6-phosphate in A. vera is discussed.

AN: 94223512

MEDLINE EXPRESS (R) 1991 - 1995 135 of 324

TI: An updated role for nerve growth factor in neurobehavioural regulation of adult vertebrates.

AU: Alleva-E; Aloe-L; Bigi-S

AD: Section of Behavioural Pathophysiology, Istituto Superiore di Sanita, Rome, Italy.

SO: Rev-Neurosci. 1993 Jan-Mar; 4(1): 41-62

This title is owned by this library

ISSN: 0334-1763

LA: ENGLISH

AB: Increasing attention has been focused on the role(s) of nerve growth factor (NGF) in neurobehavioural regulations of adult vertebrates. This interest springs from the emerging evidence that NGF is a "regulator" of physiological processes belonging to the three main homeostatic systems: the nervous, immune and endocrine systems. In fact, the spectrum of action of the NGF molecule is not restricted to neuronal cell types (central basal forebrain; peripheral sensory and sympathetic neurons) but extends also to nonneuronal cells. In mice intermale aggressive behaviour enhances serum NGF levels and promotes its synthesis in some hypothalamic areas. Other types of social events are able to cause NGF release, particularly under stress conditions. The achievement of a social role (dominant vs subordinate) is due to a functional loop involving salivary NGF release-->enhanced production of adrenal hormones-->submissive behaviour-->NGF release. In humans, plasma platelet-derived growth factor (PDGF) increases following mental stress. The aim of this review is to give an updated survey on NGF roles in neurobehavioural regulations of adult animals.

AN: 95040108

MEDLINE EXPRESS (R) 1991 - 1995 136 of 324

TI: Influence of ethanol consumption on brain nerve growth factor and its target cells in developing and adult rodents.

AU: De-Simone-R; Aloe-L

AD: Istituto di Neurobiologia, Consiglio Nazionale delle Ricerche, Rome, Italy.

SO: Ann-Ist-Super-Sanita. 1993; 29(1): 179-83

This title is owned by this library

ISSN: 0021-2571

LA: ENGLISH

AB: In the present study the effect of alcohol consumption on brain nerve growth factor (NGF) and the NGF-target cells of adult male rats and pups exposed prenatally to ethanol is evaluated. It is hypothesized that NGF, a trophic agent for the survival and maintenance of basal forebrain cholinergic neurons, might be affected by the neurodegenerative events which occur during ethanol consumption. To test this hypothesis, we used adult rats exposed to ethanol for 16 weeks and pregnant rats exposed to ethanol for six days. Our experiments show that ethanol ingestion causes a reduction of NGF in the hippocampus, of choline acetyltransferase activity in both the septum and hippocampus, and in the distribution of NGF receptor in the basal forebrain cholinergic neurons. The results indicate that the damaging effect of alcohol on forebrain cholinergic neurons is also associated with impairment of central NGF-target structures.

AN: 94175408

MEDLINE EXPRESS (R) 1991 - 1995 137 of 324

TI: Sennosides and aloin do not promote dimethylhydrazine-induced colorectal tumors in mice.

AU: Siegers-CP; Siemers-J; Baretton-G

AD: Institutes of Toxicology, Medical University of Lubeck, FRG.

SO: Pharmacology. 1993 Oct; 47 Suppl 1: 205-8

This title is owned by this library

ISSN: 0031-7012

LA: ENGLISH

AB: In a model of dimethylhydrazine-induced colorectal tumors in male mice aloin- or sennoside-enriched diets (0.03%) did not promote incidence and growth of adenomas and carcinomas after 20 weeks. Furthermore, in anthranoid-fed mice no significant changes in serum electrolytes as well as parameters of hepato- and nephrotoxicity were observed.

AN: 94052309

MEDLINE EXPRESS (R) 1991 - 1995 138 of 324

TI: Postnatal cocaine exposure affects neonatal passive avoidance performance and cholinergic development in rats.

AU: Ricceri-L; Tirassa-P; Aloe-L; Alleva-E

AD: Section of Behavioral Pathophysiology, Istituto Superiore di Sanita, Rome, Italy.

SO: Pharmacol-Biochem-Behav. 1993 Jun; 45(2): 283-9

This title is owned by this library

ISSN: 0091-3057

LA: ENGLISH

AB: Wistar rat pups received either cocaine HCl (25 mg/kg) or saline (0.9% NaCl) SC from postnatal days 1-11. On days 12 (acquisition) and 13 (retention), they underwent a passive avoidance task (step-off response; grid foot-shock at 0.35 mA). Slight deficits were found in cocaine-treated subjects for latency to step-off during acquisition and for generalized increase in the number of trials to criterion in retention. On postnatal day 13, the level of choline acetyltransferase (ChAT) enzymatic activity and the distribution of ChAT neuronal immunoreactivity in forebrain structures were examined. These morphometric and biochemical studies demonstrate a decrease of cholinergic enzymes in the septum, while the remaining basal forebrain cholinergic regions were unaffected.

AN: 93317702

MEDLINE EXPRESS (R) 1991 - 1995 139 of 324

TI: The effect of chronic ethanol intake on brain NGF level and on NGF-target tissues of adult mice.

AU: Aloe-L; Bracci-Laudiero-L; Tirassa-P

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: Drug-Alcohol-Depend. 1993 Jan; 31(2): 159-67

This title is owned by this library

ISSN: 0376-8716

LA: ENGLISH

AB: The effect of ethanol consumption on the forebrain and hypothalamus of adult mice was investigated. A consistent decrease of biological activity and of nerve growth factor (NGF) immunoreactivity was observed in the hippocampus and hypothalamus of alcohol-treated mice. Biochemical studies also indicate that chronic ethanol intake causes a reduction in the level of choline-acetyltransferase in the septum, hippocampus and striatum, but not in the cortex and other brain regions. This study provides evidence that long-term ethanol intake causes impairment of brain NGF level and of the cholinergic enzyme, regulated by NGF, suggesting that NGF synthesis and/or biological activity is affected in alcohol-related brain neuropathology.

AN: 93170126

MEDLINE EXPRESS (R) 1991 - 1995 140 of 324

TI: [The therapeutic properties of aloe]

TO: Likuval'ni vlastyvosti aloe.

AU: Iena-IaM

SO: Lik-Sprava. 1993 Feb-Mar(2-3): 142-5

ISSN: 1019-5297

LA: UKRAINIAN; NON-ENGLISH

AB: Data are reported on the use of aloes in research and folk medicine. Aloes is used in the form of dry juice of aloe leaves, fluid extract, juice, ointments. The author discusses indications and contraindications to the use of aloe. Recipes are given of mixtures with aloe which may be used in domestic conditions for increasing the defensive forces of the body during radiation lesions.

AN: 94249208

MEDLINE EXPRESS (R) 1991 - 1995 141 of 324

TI: A proposed autacoid mechanism controlling mastocyte behaviour.

AU: Aloe-L; Leon-A; Levi-Montalcini-R

AD: Institute of Neurobiology, National Research Council, Rome, Italy.

SO: Agents-Actions. 1993; 39 Spec No: C145-7

This title is owned by this library

ISSN: 0065-4299

LA: ENGLISH

AB: Evidence is provided here supporting the existence of a novel autacoid mechanism negatively modulating mast cell behaviour in response to noxious stimuli in vivo; hence, the denomination "autacoid local inflammation antagonism" (ALIA). In particular, as lipid amides of the N-acylethanolamine type have been reported to accumulate in tissues in degenerative inflammatory conditions, we examined whether these N-acylated lipids could exert regulatory effects on mast cell activation in vivo. The results reported show that both long- and short-chain N-acylethanolamines, when systemically administered, are effective in reducing mast cell degranulation induced by local injection of substance P in the ear pinna of developing rats. These and other data suggest that the endogenous production of N-acylethanolamines may constitute a local autocrine/paracrine response for the negative feedback control of mast cell responses to various activating signals. Such a process may be of physio-pathological relevance in the regulation of functional neuro-immune-mast cell interactions.

AN: 94099092

MEDLINE EXPRESS (R) 1991 - 1995 142 of 324

TI: Neonatal capsaicin exposure affects isolation-induced aggressive behavior and hypothalamic substance P levels of adult male mice (Mus musculus).

AU: Bigi-S; De-Acetis-L; De-Simone-R; Aloe-L; Alleva-E

AD: Section of Behavioral Pathophysiology, Istituto Superiore di Sanita, Rome, Italy.

SO: Behav-Neurosci. 1993 Apr; 107(2): 363-9

This title is owned by this library

ISSN: 0735-7044

LA: ENGLISH

AB: Subcutaneous administration of capsaicin (50 mg/kg) at Postnatal Days 2 and 5 exerted long-term effects on isolation-induced aggressive behavior of adult mice (Mus musculus) of the CD-1 strain. Isolated capsaicin-treated mice (scored during a 10-min session) showed the highest frequency and the longest duration of total attacks, attacks, rattling, and offensive upright posture when compared with nonisolated capsaicin-treated subjects and both isolated and nonisolated vehicle control animals. Hypothalamic Substance P (SP) was assessed by radioimmunoassay. Capsaicin treatment significantly lowered hypothalamic SP content in both isolated and nonisolated mice. Moreover, individual scores of isolated capsaicin-treated subjects showed a significant correlation between SP depletion and expression of offensive upright posture. Isolation per se was revealed to play an important role in depleting SP from the hypothalamus.

AN: 93249647

MEDLINE EXPRESS (R) 1991 - 1995 143 of 324

TI: Components of the gel of Aloe vera (L.) burm. f.

AU: Yamaguchi-I; Mega-N; Sanada-H

AD: Department of Nutrition, Faculty of Home Economics, Tokyo Kasei University, Japan.

SO: Biosci-Biotechnol-Biochem. 1993 Aug; 57(8): 1350-2

This title is owned by this library

ISSN: 0916-8451

LA: ENGLISH

AN: 93379359

MEDLINE EXPRESS (R) 1991 - 1995 144 of 324

TI: Polycythaemia vera and cerebral blood flow: a preliminary study with transcranial Doppler.

AU: Fiermonte-G; Aloe-Spiriti-MA; Latagliata-R; Petti-MC; Giacomini-P

AD: Institute of Clinical Nervous and Mental Diseases, La Sapienza University, Rome, Italy.

SO: J-Intern-Med. 1993 Dec; 234(6): 599-602

This title is owned by this library

ISSN: 0954-6820

LA: ENGLISH

AB: OBJECTIVES. The purpose of this study has been to investigate by ultrasonographic methods the flow velocities of cerebral arteries because increased blood viscosity due to haematocrit elevation can cause neurological symptoms in polycythaemia vera patients, because of the resulting decrease in cerebral flow. SUBJECTS AND DESIGN. Twenty newly diagnosed patients, with haemoglobin values of > 18 g dl-1 and/or an haematocrit of > 50%, were examined by transcranial Doppler. Recordings were performed in basal conditions and after pharmacological and/or phlebotomic treatment, when haematocrit values were < or = 50%. Blood velocities were evaluated in middle (MCA), anterior (ACA), posterior (PCA) cerebral arteries and in the basilar (BA) artery. RESULTS. Basal recordings showed decreased velocities (MCA: 39.40 +/- 9.34 cm s-1; ACA: 34.05 +/- 10.25 cm s-1; PCA: 31.46 +/- 5.97 cm s-1; and BA: 27.47 +/- 7.42 cm s-1); pre- and post-treatment value differences observed in MCA, ACA and BA were highly significant (P < 0.001). CONCLUSIONS. A decrease in cerebral flow could be a risk for multifocal micro-ischaemic cerebral infarctions leading, after several years, to a multi-infarct dementia; an early reduction in erythrocyte burden should be very useful in polycythaemic patients in preventing lacunar lesions.

AN: 94081416

MEDLINE EXPRESS (R) 1991 - 1995 145 of 324

TI: [The molecular structure of iso-aloesin isolated from the leaves of Aloe vera L. var. chinensis (Haw.) Berge]

AU: Yuan-AX

AD: Guanxi Institute of Traditional Chinese Medical and Pharmaceutical Sciences, Nanning.

SO: Chung-Kuo-Chung-Yao-Tsa-Chih. 1993 Oct; 18(10): 609-11, 639

ISSN: 1001-5302

LA: CHINESE; NON-ENGLISH

AB: A new constituent iso-aloesin was isolated from the leaves of Aloe vera var. chinensis found in the Province of Guangxi. The molecular formula of iso-aloesin is C19H22O9, which is 2-acetonyl-6-C-beta-D-glucopyranosyl-7-hydroxy-5-methyl-chromone.

AN: 94271443

MEDLINE EXPRESS (R) 1991 - 1995 146 of 324

TI: Purification and characterization of a glutathione peroxidase from the Aloe vera plant.

AU: Sabeh-F; Wright-T; Norton-SJ

AD: Department of Biological Sciences, University of North Texas, Denton 76201.

SO: Enzyme-Protein. 1993; 47(2): 92-8

This title is owned by this library

ISSN: 1019-6773

LA: ENGLISH

AB: Extracts from the parenchymous leaf-gel of the Aloe vera plant (Aloe barbadensis Miller) were shown to contain glutathione peroxidase (GSHPx) activity. The activity was purified to homogeneity by ion exchange and gel filtration (FPLC) chromatography in the presence of 0.5 mM glutathione. The native enzyme has an apparent molecular weight of 62 kD as determined by gel filtration. In the presence of sodium dodecylsulfate (SDS), the molecular weight was estimated to be about 16 kD as determined by polyacrylamide-gel electrophoresis (SDS-PAGE). The native enzyme is proposed to be constituted of four identical subunits; it also contains one atom of selenium per subunit, as found with most glutathione peroxidases from animal sources. The Km values were determined to be 3.2 mM for glutathione and 0.26 mM for the hydroperoxide substrate, cumene hydroperoxide. The enzyme is competitively inhibited by N, S, bis-FMOC glutathione (Ki = 0.32 mM), a potent inhibitor of glyoxalase II. Inhibitors of glyoxalase I (e.g. S-octylglutathione) have no effect on the peroxidase activity.

AN: 94251296

MEDLINE EXPRESS (R) 1991 - 1995 147 of 324

TI: Level of nerve growth factor and distribution of mast cells in the synovium of tumour necrosis factor transgenic arthritic mice.

AU: Aloe-L; Probert-L; Kollias-G; Bracci-Laudiero-L; Micera-A; Mollinari-C; Levi-Montalcini-R

AD: Institute of Neurobiology, Rome, Italy.

SO: Int-J-Tissue-React. 1993; 15(4): 139-43

This title is owned by this library

ISSN: 0250-0868

LA: ENGLISH

AB: We have recently reported that nerve growth factor (NGF) increases in the synovium of patients affected by arthritis, as well as in animal models. We report here that the synovium of transgenic arthritic mice expressing human tumour necrosis factor (TNF) contains numerous mast cells (MC) and that their appearance is a phenomenon which was correlated to the local increase in NGF level. These findings provide further evidence that NGF plays a role in inflammation and suggest a functional link between NGF and MC.

AN: 94245446

MEDLINE EXPRESS (R) 1991 - 1995 148 of 324

TI: Erythropoietin treatment of idiopathic myelofibrosis.

AU: Aloe-Spiriti-M; Latagliata-R; Avvisati-G; Battistel-V; Montefusco-E; Spadea-A; Petti-MC

AD: Department of Human Biopathology, University La Sapienza of Roma, Italy.

SO: Haematologica. 1993 Nov-Dec; 78(6): 371-3

This title is owned by this library

ISSN: 0390-6078

LA: ENGLISH

AB: BACKGROUND. In order to determine whether recombinant human erythropoietin (rHuEPO) may play a role in treating anemia in idiopathic myelofibrosis (IMF), a pilot study using high doses of rHuEPO was conducted on patients with IMF. METHODS AND RESULTS. From September, 1990 to December, 1992, 7 patients (6 males and 1 female, median age 68 years) affected by IMF entered the trial. RHuEPO was administered subcutaneously 5 days a week at a dosage of 160 U/kg daily for three months. Out of 7 patients, 4 obtained a response. These responders received additional maintenance treatment with rHuEPO until relapse. Response duration was 6, 7, 10+, and 16 months, respectively. Treatment was well-tolerated. CONCLUSIONS. Our data suggest that rHuEPO may play a role in the treatment of anemia in some IMF patients.

AN: 94229629

MEDLINE EXPRESS (R) 1991 - 1995 149 of 324

TI: Anthranoid laxative abuse--a risk for colorectal cancer?

AU: Siegers-CP; von-Hertzberg-Lottin-E; Otte-M; Schneider-B

AD: Institute of Toxicology, Medical University of Lubeck, Germany.

SO: Gut. 1993 Aug; 34(8): 1099-101

This title is owned by this library

ISSN: 0017-5749

LA: ENGLISH

AB: Anthranoid-containing laxatives--aloe, cascara, frangula, and rheum--may play a role in colorectal cancer. This risk is particularly important in view of the wide abuse of self administered laxatives for chronic constipation. There are data on the genotoxic potential of anthranoids and there is evidence of a tumourigenic potential in rodents. A case report and clinical-epidemiological studies have evaluated the cancer risk in patients who have abused anthranoid laxatives over a long period. Pseudomelanosis coli is a reliable parameter of chronic laxative abuse (> 9-12 months) and is specific for anthranoid drugs. In a retrospective study of 3049 patients who underwent diagnostic colorectal endoscopy the incidence of pseudomelanosis coli was 3.13% in patients without pathological changes. In those with colorectal adenomas, the incidence increased to 8.64% (p < 0.01), and in those with colorectal carcinomas it was 3.29%. This lower rate was probably caused by incomplete documentation of pseudomelanosis coli in those with carcinoma. In a prospective study of 1095 patients, the incidence of pseudomelanosis coli was 6.9% for patients with no abnormality seen on endoscopy, 9.8% (p = 0.068) for patients with adenomas, and 18.6% for patients with colorectal carcinomas. From these data a relative risk of 3.04 (1.18, 4.90; 95% confidence interval) can be calculated for colorectal cancer as a result of anthranoid laxative abuse.

AN: 94229551

MEDLINE EXPRESS (R) 1991 - 1995 150 of 324

TI: Identification of natural antimutagens with modulating effects on DNA repair.

AU: Vukovic-Gacic-B; Simic-D

AD: Botanical Institute and Garden, University of Belgrade, Yugoslavia.

SO: Basic-Life-Sci. 1993; 61: 269-77

This title is owned by this library

ISSN: 0090-5542

LA: ENGLISH

AB: The results of a study of bioantimutagenesis, with emphasis on natural antimutagens from plant extracts with modulating effects on DNA repair in Escherichia coli bacteria are presented in this chapter. Comparative screening for spontaneous or induced mutagenesis, as well as expression of the SOS gene, sfiA was accomplished. Antimutagenic capacity was obtained with nontoxic concentrations of the plant extracts; the same plant extract may decrease or increase the mutation rate, or even be ineffective, depending on the bacterial strain used and the concentration of the extract applied. Since antimutagenic effects may be the consequence of either stimulation of error-free repair, inhibition of error-prone repair, or involvement of multiple mechanisms, the effects of several plant extracts on the level of UV-induced beta-galactosidase were screened (to monitor SOS induction in cells). Reduction of the enzyme activity induced by UV was observed following addition of St. John's wort extract, while there was not reduction after thyme, aloe, camomile, or lime-tree and the level of UV-induced enzyme was even higher with sage extract. Our results indicate that the antimutagenic effect of St. John's wort is probably due to suppression of error-prone repair. Moreover, we assume that an antimutagenic effect obtained with thyme, mint, and sage under certain conditions may be due to enhanced error-free repair.

AN: 94137240

MEDLINE EXPRESS (R) 1991 - 1995 151 of 324

TI: Immunohistochemical localization of nerve growth factor (NGF) and NGF-receptor in the hypothalamus of adult rats.

AU: Talamini-LM; Aloe-L

AD: Institute of Neurobiology, CNR, Roma, Italy.

SO: Arch-Ital-Biol. 1993 Sep; 131(4): 255-66

This title is owned by this library

ISSN: 0003-9829

LA: ENGLISH

AB: In the adult mammalian central nervous system (CNS) the nerve growth factor (NGF) has thus far been associated mainly with the metabolic support and pathophysiology of cholinergic basal forebrain (CBF) neurons. Recently, however, the presence of NGF and NGF mRNA has been demonstrated in the hypothalamus of adult mice. In addition there have been reports on the stimulation of the pituitary-adrenocortical axis by NGF. These and other findings suggest the involvement of NGF in hypothalamic functioning. The aim of the present study was to improve immunohistochemical methodologies for the investigation of NGF and NGF-receptor (NGF-r) in the hypothalamus of adult rats. Our results show NGF and NGF-r expression in various regions of the hypothalamus. Moreover, in some areas, distribution and morphology of immunoreactive neurons suggest neuronal colocalization of the two markers. To study the function of NGF in the hypothalamus, anti-NGF antibody was injected in the area of the paraventricular nucleus. We did not, however, find an effect of treatment on NGF-r immunoreactive neurons in this area.

AN: 94071578

MEDLINE EXPRESS (R) 1991 - 1995 152 of 324

TI: NGF is released into plasma during human pregnancy: an oxytocin-mediated response?

AU: Luppi-P; Levi-Montalcini-R; Bracci-Laudiero-L; Bertolini-A; Arletti-R; Tavernari-D; Vigneti-E; Aloe-L

AD: Institute of Neurobiology, C.N.R., Roma, Italy.

SO: Neuroreport. 1993 Aug; 4(8): 1063-5

ISSN: 0959-4965

LA: ENGLISH

AB: The presence of biologically active nerve growth factor (NGF) in the peripheral circulation of women during pregnancy, labour and lactation was investigated. Using a sensitive immunoenzymatic assay (ELISA), we found an approximately five-fold increase in plasma NGF levels during labour and lactation compared with the concentrations found at the term of gestation or in control healthy women. Since labour and lactation are characterized by activation of the hypothalamo-pituitary-adrenal axis and by high plasma levels of the neurohypophyseal hormone oxytocin, and since the intravenous injection of oxytocin in female rats causes a 176% increase in the hypothalamic levels of NGF, it is possible that the increased amount of circulating NGF is correlated with one or both of these events.

AN: 94060318

MEDLINE EXPRESS (R) 1991 - 1995 153 of 324

TI: Analytics of senna drugs with regard to the toxicological discussion of anthranoids.

AU: Grimminger-W; Witthohn-K

AD: Chemical Research Department, Madaus AG, Koln, FRG.

SO: Pharmacology. 1993 Oct; 47 Suppl 1: 98-109

This title is owned by this library

ISSN: 0031-7012

LA: ENGLISH

AB: Toxicological studies indicate that two hydroxyanthraquinones (HAs), aloe-emodin and emodin, present as minor components in senna, might represent a genotoxic or cancerogenetic risk for man. Since aloe-emodin and emodin occur in senna in the free form as well as their glucosides and dianthrone glucosides, a HPLC method was established to allow the quantification of all free and glycosidic 1,8-dihydroxy anthranoids. The sum of the free HAs and their calculated content in each of their prodrug forms is defined as the potential HA content. For the comparison of different senna drugs in respect to the genotoxic risk arising from their potential aloe-emodin or emodin contents, a risk index has been established.

AN: 94052328

MEDLINE EXPRESS (R) 1991 - 1995 154 of 324

TI: Antioxidant and radical scavenging effects of anthraquinones and anthrones.

AU: Malterud-KE; Farbrot-TL; Huse-AE; Sund-RB

AD: Department of Pharmacognosy, University of Oslo, Norway.

SO: Pharmacology. 1993 Oct; 47 Suppl 1: 77-85

This title is owned by this library

ISSN: 0031-7012

LA: ENGLISH

AB: The activity of seven anthraquinones and four anthrones against nonenzymatic and enzymatic lipid peroxidation in vitro and their ability to scavenge free radicals have been studied. In nonenzymatic peroxidation in rat hepatocytes induced by t-butyl hydroperoxide, dithranol and anthrone were the strongest antioxidants, having IC50 values of 8 +/- 1 and 24 +/- 5 mumol/l, respectively. Rhein (IC50 64 +/- 2 mumol/l) and aloe-emodin (IC50 65 +/- 3 mumol/l) showed the highest inhibitory activity against peroxidation of linoleic acid catalyzed by soybean 15-lipoxygenase. Anthrone (IC50 62 +/- 2 mumol/l), dithranol (IC50 72 +/- 1 mumol/l) and rhein anthrone (IC50 76 +/- 6 mumol/l) were the most effective radical scavengers of the diphenylpicrylhydrazyl radical. The antioxidant activities in hepatocytes and the radical scavenging activities were correlated, whereas the inhibition of enzymatic lipid peroxidation showed no correlation with the two other effects.

AN: 94052326

MEDLINE EXPRESS (R) 1991 - 1995 155 of 324

TI: The senna drug and its chemistry.

AU: Franz-G

AD: Department of Pharmacy, University of Regensburg, FRG.

SO: Pharmacology. 1993 Oct; 47 Suppl 1: 2-6

This title is owned by this library

ISSN: 0031-7012

LA: ENGLISH

AB: Senna consists of the dried leaflets or fruits of Cassia senna (C. acutifolia) known in commerce as Alexandrian senna and of Cassia angustifolia commonly known as Tinnevelly senna. The senna plants are small shrubs of Leguminosae cultivated either in Somalia, the Arabian peninsula and near the Nile river. Tinnevelly senna is obtained from cultivated plants mainly in South India and Pakistan. Owing to the careful way in which the plant is harvested, the leaflets of the drug are usually little broken. Damaged leaves and lower quality products are often used for making galenicals. The senna pods (fruits) are collected during the same period as the leaves, then dried and separated into various qualities. The active principle of Senna was first isolated and characterized by Stoll in 1941. The first two glycosides were identified and attributed to the anthraquinone family. These were found to be dimeric products of aloe emodin and/or rhein which were named sennoside A and sennoside B. They both hydrolyze to give the aglycones sennidin A and B and two molecules of glucose. Later work confirmed these findings and further demonstrated the presence of sennosides C and D. Small quantities of monomeric glycosides and free anthraquinones seem to be present as well. The active constituents of the pods are similar to those of the leaves but present in larger quantities. Two naphthalene glycosides isolated from senna leaves and pods are 6-hydroxymusicin glucoside and tinnevellin glucoside.Both compounds can be utilized to distinguish between the Alexandrian senna and the India senna, since tinnevellin glucoside is only found in the latter and the first only in the C. senna.(ABSTRACT TRUNCATED AT 250 WORDS)

AN: 94052308

MEDLINE EXPRESS (R) 1991 - 1995 156 of 324

TI: The genotoxicity status of senna.

AU: Heidemann-A; Miltenburger-HG; Mengs-U

AD: CCR Cytotest Cell Research, Rossdorf, FRG.

SO: Pharmacology. 1993 Oct; 47 Suppl 1: 178-86

This title is owned by this library

ISSN: 0031-7012

LA: ENGLISH

AB: Genotoxicity tests were performed by several laboratories with the drug fructus sennae, senna extract, sennosides, rhein and aloe-emodin. The drug fructus sennae, the sennosides and rhein did not increase mutation frequencies in the following test systems: bacterial systems (Salmonella reverse mutation test and/or Escherichia coli forward mutation test); mammalian cell cultures [hypoxanthine guanine phosphoribosyl transferase (HGPRT) test; mouse lymphoma test; chromosome aberration test with Chinese hamster ovary cells]; bone marrow (micronucleus test; chromosome aberration test); melanoblast cells (mouse spot test) of rodents. With aloe-emodin mutagenic effects were observed only in vitro in the chromosome aberration test with CHO cells and in the Salmonella reverse mutation test (frameshift mutations in strains TA 1537, TA 1538 and TA 98). In the in vitro gene mutation test with V79 cells (HGPRT test) no mutagenic potential of aloe-emodin was observed. In in vivo studies [micronucleus test with bone marrow cells of NMRI mice, chromosome aberration test with bone marrow cells of Wistar rats, mouse spot test (crossing DBA/2J x NMRI) no indication for a mutagenic activity of aloe-emodin was found. The relevance of the absence of a mutagenic potential in in vivo test systems was strengthened by the fact that aloe-emodin could be found in the blood serum after oral administration. Additional information on the interaction of aloe-emodin with DNA was obtained from an ex vivo unscheduled DNA synthesis test performed with hepatocytes of male Wistar rats: aloe-emodin did not induce unscheduled DNA synthesis as expression of DNA damage.(ABSTRACT TRUNCATED AT 250 WORDS)

AN: 94052305

MEDLINE EXPRESS (R) 1991 - 1995 157 of 324

TI: Cleavages of the O- and C-glucosyl bonds of anthrone and 10,10'-bianthrone derivatives by human intestinal bacteria.

AU: Hattori-M; Akao-T; Kobashi-K; Namba-T

AD: Research Institute for Wakan-Yaku (Traditional Sino-Japanese Medicines), Toyama Medical and Pharmaceutical University.

SO: Pharmacology. 1993 Oct; 47 Suppl 1: 125-33

This title is owned by this library

ISSN: 0031-7012

LA: ENGLISH

AB: A strictly anaerobic bacterium, Bifidobacterium sp. SEN, capable of hydrolyzing the O-glucosyl of sennosides was isolated from human feces. The bacterium stepwisely hydrolyzed sennoside B to sennidin B through sennidin-8-monoglucoside in PYF medium but not in GAM broth. Addition of D-glucose to PYF medium resulted in loss of the hydrolyzing activity in culture but addition of D-fructose did not affect the activity. Coculture of this bacterium with Peptostreptococcus intermedius led to rapid accumulation of rhein anthrone in the medium. Similarly, a bacterium, Eubacterium sp. BAR, capable of cleaving the C-glucosyl of barbaloin was isolated from human feces. This bacterium grew in PYF medium containing barbaloin and produced enzyme(s) that cleave(s) the C-glucosyl. The induction of the enzymes was completely inhibited in the presence of D-glucose. Nojirimycin inhibited the enzyme activity induced by barbaloin but it did not inhibit the bacterial growth in the presence of D-glucose.

AN: 94052297

MEDLINE EXPRESS (R) 1991 - 1995 158 of 324

TI: Rhein and aloe-emodin kinetics from senna laxatives in man.

AU: Krumbiegel-G; Schulz-HU

AD: Department of Pharmacokinetics, Madaus AG, Koln, FRG.

SO: Pharmacology. 1993 Oct; 47 Suppl 1: 120-4

This title is owned by this library

ISSN: 0031-7012

LA: ENGLISH

AB: Therapeutic doses of two laxatives (Agiolax and Sennatin) were repeatedly administered to 10 healthy volunteers in a two-way change-over design. Blood samples were collected up to 96 h after the first dose, and plasma levels of total aloe-emodin and rhein were determined simultaneously with a sensitive (lower limit of quantification: 0.5 ng aloe-emodin and 2.5 ng rhein per millilitre plasma) and specific fluorometric HPLC method. Aloe-emodin was not detectable in any plasma sample of any subject. Rhein concentration time courses showed highest levels of 150-160 ng/ml and peak maxima at 3-5 h and 10-11 h after dosing probably according to absorption of free rhein and rhein released from prodrugs (e.g. sennosides) by bacterial metabolism, respectively.

AN: 94052296

MEDLINE EXPRESS (R) 1991 - 1995 159 of 324

TI: Pharmacokinetic-metabolic studies with 14C-aloe emodin after oral administration to male and female rats.

AU: Lang-W

AD: Department of Radiobiochemistry, Madaus AG, Koln, FRG.

SO: Pharmacology. 1993 Oct; 47 Suppl 1: 110-9

This title is owned by this library

ISSN: 0031-7012

LA: ENGLISH

AB: After oral administration of 4.5 mg/kg 14C-aloe emodin (AE) to rats 20-30% of the dose was excreted in urine and the rest in feces. AE was quickly metabolized to rhein, to an unknown metabolite and to conjugates of all three. In the plasma about 10% of 14C-activity was identified as free AE. Maximum plasma values were reached 1.5-3 h p.a. with 248 (male) and 441 (female) ng equivalents AE/ml. Maximum concentrations in plasma were about 3 times higher than those in ovaries and 10 times higher than those in testes. Only liver, kidney and intestinal tract showed higher concentrations than plasma. Terminal half-life (for radioactivity) in blood was about 50 h.

AN: 94052295

MEDLINE EXPRESS (R) 1991 - 1995 160 of 324

TI: The synovium of transgenic arthritic mice expressing human tumor necrosis factor contains a high level of nerve growth factor.

AU: Aloe-L; Probert-L; Kollias-G; Bracci-Laudiero-L; Spillantini-MG; Levi-Montalcini-R

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: Growth-Factors. 1993; 9(2): 149-55

This title is owned by this library

ISSN: 0897-7194

LA: ENGLISH

AB: We have recently reported that nerve growth factor (NGF) increases in the synovium of patients affected by rheumatoid arthritis and in the synovium of pharmacologically-induced arthritis in animal models. In the present study, we demonstrate that arthritic transgenic mice which carry and express the human TNF gene (Tg197) also express elevated levels of NGF, and that subcutaneous injection of NGF-antibodies attenuates the loss of body weight caused by the development of disease in these mice. Along with our previous findings, which show an increase in the level of NGF during the acute phase of other autoimmune diseases, these results suggest a role of NGF in these pathologies. The functional significance of NGF in rheumatoid arthritis (RA) is currently under study.

AN: 94031113

MEDLINE EXPRESS (R) 1991 - 1995 161 of 324

TI: Production and characterization of a monoclonal antibody against nerve growth factor (NGF) which recognizes rodent and human NGF.

AU: Vigneti-E; Bracci-Laudiero-L; Aloe-L

AD: Institute of Cell Biology, CNR, Rome, Italy.

SO: Year-Immunol. 1993; 7: 146-9

This title is owned by this library

ISSN: 0256-2308

LA: ENGLISH

AN: 93383479

MEDLINE EXPRESS (R) 1991 - 1995 162 of 324

TI: NGF retards apoptosis in chick embryo bursal cell in vitro.

AU: Bracci-Laudiero-L; Vigneti-E; Iannicola-C; Aloe-L

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: Differentiation. 1993 Jun; 53(2): 61-6

This title is owned by this library

ISSN: 0301-4681

LA: ENGLISH

AB: Recent studies have demonstrated that the action of nerve growth factor (NGF) is not restricted to neuronal cells but also affects cells of the immune system. In a previous work on the effect of NGF on the chick embryo bursa of Fabricius both in vivo and in vitro, we observed that NGF prolongs bursal cell survival in vitro. In the present study we report that the increase of viable cells in NGF-treated cultures is not due to a proliferative effect of NGF on bursal cells but to a reduction of cell mortality. The morphological analysis revealed that bursal cells in cultures die by apoptosis, which was also shown by the typical pattern of DNA fragmentation, a hallmark of this cell death process. It is concluded that NGF, with an action similar to that described in sympathetic neurons and PC12, could retard bursal cell death by influencing apoptosis.

AN: 93366032

MEDLINE EXPRESS (R) 1991 - 1995 163 of 324

TI: Nerve growth factor and mast cell distribution in the skin of patients with systemic sclerosis.

AU: Tuveri-MA; Passiu-G; Mathieu-A; Aloe-L

AD: II Chair of Rheumatology, University of Cagliari, Italy.

SO: Clin-Exp-Rheumatol. 1993 May-Jun; 11(3): 319-22

This title is owned by this library

ISSN: 0392-856X

LA: ENGLISH

AB: Nerve growth factor (NGF), a well-characterized neurotrophic factor, induces an increase in the number of mast cells (MCs) in the peripheral tissues of developing rats, as well as histamine release in fully differentiated MCs. Since MCs increase in the dermis of patients with early systemic sclerosis (SSc), we examined the distribution of NGF in the skin of patients affected by SSc. Immunohistochemical studies showed that NGF distribution was more intense in the dermis of patients with SSc than in the skin of controls. A possible correlation between MCs and NGF in disease activity is hypothesized.

AN: 93358534

MEDLINE EXPRESS (R) 1991 - 1995 164 of 324

TI: Recombinant human erythropoietin in the treatment of myelodysplastic syndromes. An interim report.

AU: Aloe-Spiriti-MA; Petti-MC; Latagliata-R; Avvisati-G; De-Gregoris-C; Proia-S; Fazi-P; Jaalouk-G; Mancini-M; Spadea-A; et-al

AD: Dipartimento di Biopatologia Umana, Universita La Sapienza, Roma, Italy.

SO: Haematologica. 1993 Mar-Apr; 78(2): 123-6

This title is owned by this library

ISSN: 0390-6078

LA: ENGLISH

AB: BACKGROUND. It has recently been demonstrated that erythropoietin increases hemoglobin levels in anemia secondary to chronic renal failure. Some recent experiences have suggested a possible role in the treatment of anemia in patients with myelodysplastic syndrome (MDS). METHODS AND RESULTS. From April, 1990 to March, 1991, 16 patients (11 males and 5 females, median age 58.5 years) affected by low-risk myelodysplastic syndromes (MDS) were treated with recombinant human erythropoietin (rHuEPO) to ameliorate Hb levels and reduce transfusional requirement. All patients received high doses of rHuEPO (400 U/Kg s.c. twice weekly for 3 months). A partial response, defined as a stable increase in Hb levels > 1g/dL and/or a reduction in transfusional need > 50% lasting at least 3 months, was achieved by 5/16 patients. Those who responded received an additional course of treatment with rHuEPO at an increased dosage (600 U/Kg twice weekly for 3 months), and one of these five showed a progressive rise in Hb level up to normalization, while the other 4 remained stable. The treatment was well tolerated and no adverse reactions were observed. CONCLUSIONS. These results suggest that some patients with MDS may benefit from rHuEPO treatment.

AN: 93351953

MEDLINE EXPRESS (R) 1991 - 1995 165 of 324

TI: Increased levels of NGF in sera of systemic lupus erythematosus patients.

AU: Bracci-Laudiero-L; Aloe-L; Levi-Montalcini-R; Galeazzi-M; Schilter-D; Scully-JL; Otten-U

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: Neuroreport. 1993 May; 4(5): 563-5

ISSN: 0959-4965

LA: ENGLISH

AB: Using a specific enzyme-linked immunosorbent assay (ELISA) for human nerve growth factor (NGF), serum levels in patients with systemic lupus erythematosus (SLE) were measured. We found a consistent increase in NGF levels in SLE patients compared with controls. A good correlation exists between serum NGF level and severity of clinical manifestation. We hypothesize that NGF might play a role in the pathogenesis of autoimmune disorders such as SLE.

AN: 93291431

MEDLINE EXPRESS (R) 1991 - 1995 166 of 324

TI: [The effect of heparin treatment on the rose bengal test for Brucella (letter)]

TO: Influencia del tratamiento con heparina sobre la prueba del rosa de Bengala para Brucella.

AU: Zancada-Diaz-de-Entre-Sotos-F; Perez-Aloe-Mejias-MT; Roldan-Montaud-A; Gomez-Pastor-AM

SO: Rev-Clin-Esp. 1993 Mar; 192(5): 247

This title is owned by this library

ISSN: 0014-2565

LA: SPANISH; NON-ENGLISH

AN: 93248454

MEDLINE EXPRESS (R) 1991 - 1995 167 of 324

TI: Is recombinant human erythropoietin treatment in myelodysplastic syndromes worthwhile?

AU: Aloe-Spiriti-MA; Petti-MC; Latagliata-R; Avvisati-G; De-Gregoris-C; Proia-S; Fazi-P; Jaalouk-G; Mancini-M; Spadea-A; et-al

AD: Department of Human Biopathology, University La Sapienza, Rome, Italy.

SO: Leuk-Lymphoma. 1993 Jan; 9(1-2): 79-83

This title is owned by this library

ISSN: 1042-8194

LA: ENGLISH

AB: It has been recently demonstrated that erythropoietin increases the haemoglobin levels in anemia secondary to chronic renal failure. Moreover some recent experiences also suggested a possible role in the treatment of MDS. From April 1990 to April 1992, 23 patients (16 males and 7 females, median age 63.5 years) affected with low risk myelodysplastic syndrome (MDS) were treated with recombinant human erythropoietin (rHuEPO) to ameliorate Hb levels and transfusional requirement. All patients received high doses of rHuEPO (800 U/Kg weekly s.c. in 2-3 divided doses, for 3 months). A complete remission, defined as stable normalization of Hb level, was achieved in 1/23 patients. This patient had refractory anemia, by FAB criteria. A partial response, defined as stable increase of Hb levels > or = 1 g/dl and/or reduction of transfusional requirement > or = 50% lasting at least 3 months, was achieved in 7/23 patients. Patients with a partial response received rHuEPO at increased dosages (1200 U/Kg weekly s.c. 2-3 times): 1/7 achieved a complete response, 4/7 remained stable and 2/7 decreased to pre-therapy Hb value. These results suggest that rHuEPO may be a promising therapeutic tool for some MDS patients.

AN: 93237816

MEDLINE EXPRESS (R) 1991 - 1995 168 of 324

TI: Determination of the position of the O-acetyl group in a beta-(1-->4)-mannan (acemannan) from Aloe barbardensis Miller.

AU: Manna-S; McAnalley-BH

AD: Synthetic Organic Division, Carrington Laboratories, Inc., Dallas, TX 75356-9500.

SO: Carbohydr-Res. 1993 Mar 17; 241: 317-9

This title is owned by this library

ISSN: 0008-6215

LA: ENGLISH

AN: 93230529

MEDLINE EXPRESS (R) 1991 - 1995 169 of 324

TI: Mitoxantrone, etoposide and intermediate-dose Ara-C (MEC): an effective regimen for poor risk acute myeloid leukemia.

AU: Spadea-A; Petti-MC; Fazi-P; Vegna-ML; Arcese-W; Avvisati-G; Aloe-Spiriti-MA; Latagliata-R; Meloni-G; Testi-AM; et-al

AD: Department of Human Biopathology, University La Sapienza, Rome, Italy.

SO: Leukemia. 1993 Apr; 7(4): 549-52

This title is owned by this library

ISSN: 0887-6924

LA: ENGLISH

AB: A total of 74 patients with poor risk AML (median age 36.7 years, range 4.5-60.6) received a single course of a regimen including mitoxantrone (6 mg/m2 intravenous bolus daily, days 1 to 6), etoposide (80 mg/m2 intravenous over 1 h, daily, days 1 to 6) and intermediate-dose Ara-C (1 g/m2 over 6 h, daily days 1 to 6). 28 patients had failed initial remission induction with daunorubicin and conventional doses of Ara-C, 16 patients had secondary AML and 30 patients had relapsed from initial remission (five within six months, 15 over six months and ten after autologous or allogeneic bone marrow transplantation). Overall 41/74 patients (55%) achieved complete remission, 26 (35%) had resistant disease and seven (10%) died of infection during marrow hypoplasia. A 4-day course of the same regimen was given as consolidation to patients in complete remission. Subsequent antileukemic therapy was individualized. Profound myelosuppression and pancytopenia were universal resulting in fever or documented infections in almost 100% of patient; major hemorrhagic complications occurred in 39% of patients. Extrahematologic toxicity was mild to moderate consisting mostly of nausea and vomiting, oral mucositis and transient liver and cardiac dysfunction. We conclude that the MEC combination chemotherapy program seems to be an effective antileukemic regimen for secondary and advanced AML, with acceptable toxicity.

AN: 93218272

MEDLINE EXPRESS (R) 1991 - 1995 170 of 324

TI: Laryngeal brain stem evoked response in the porcine model.

AU: Cohen-S; Esclamado-RM; Telian-S; Aloe-L; Kileny-P

AD: Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor 48109-0312.

SO: Ann-Otol-Rhinol-Laryngol. 1993 Jan; 102(1 Pt 1): 28-34

This title is owned by this library

ISSN: 0003-4894

LA: ENGLISH

AB: Exaggeration of normally protective laryngeal reflexes is thought to play a role in several disorders, including the sudden infant death syndrome. An analysis of brain stem neural activity following laryngeal stimulation may provide insight into the pathophysiology of pathologic laryngeal reflexes and help to identify individuals at risk for these disorders. The purpose of this study was to define the far-field brain stem activity following laryngeal stimulation in the porcine model. This activity has been termed the laryngeal brain stem evoked response and may represent a potentially useful and objective measure of the neuronal activity in the laryngeal reflex pathway. Electrical stimulation of the superior laryngeal nerve was performed in 14 mixed-breed piglets under a variety of physiologic conditions. A total of six positive and six negative discrete waves were detected, with mean latencies ranging from 1.24 to 7.16 milliseconds. Stimulations performed during hypoxic, hypercapneic, or hypocapneic conditions resulted in no significant differences in waveform latencies. There appears to be a reproducible, but somewhat variable, brain stem response elicited by superior laryngeal nerve stimulation that can be recorded via a far-field technique in the porcine model.

AN: 93128825

MEDLINE EXPRESS (R) 1991 - 1995 171 of 324

TI: The effect of long-term alcohol intake on brain NGF-target cells of aged rats.

AU: Aloe-L; Tirassa-P

AD: Instituto di Neurobiologia, Consiglio Nazionale delle Ricerche, Rome, Italy.

SO: Alcohol. 1992 Jul-Aug; 9(4): 299-304

ISSN: 0741-8329

LA: ENGLISH

AB: It was reported that chronic exposure to ethanol causes a loss of hippocampal pyramidal cells and of brain cholinergic neurons in both laboratory animals and humans. In the present study, it was hypothesized that nerve growth factor (NGF), a trophic agent for the survival and maintenance of basal forebrain cholinergic neurons (FCN), might be affected by the neurodegenerative events which occur during ethanol consumption. To test this hypothesis, we used aged rats (14 months) exposed for 16 weeks to 40 g/kg per day of undiluted wine. Our experiments showed that chronic alcohol consumption causes a reduction of NGF in the hippocampus (HI) and of choline acetyltransferase (ChAT) activity in both the septum and the HI and a reduction in the distribution of NGF-receptors (NGF-R) in the septum and nucleus of Meynert. Intracerebral injection of NGF in alcohol-exposed rats results in a return to normal levels of ChAT enzymatic activity and NGF-R expression. These experiments indicate that the damaging effect of alcohol on the FCN is also associated with impairment of central NGF-target structures.

AN: 92344751

MEDLINE EXPRESS (R) 1991 - 1995 172 of 324

TI: Unbalanced 6p translocation as primary karyotypic anomaly in secondary acute nonlymphocytic leukemia.

AU: Mancini-M; Mecucci-C; Cedrone-M; Rondinelli-MB; Aloe-Spiriti-MA; Alimena-G

AD: Hematology, Department of Human Biopathology, University of Rome La Sapienza, Italy.

SO: Cancer-Genet-Cytogenet. 1992 May; 60(1): 93-5

This title is owned by this library

ISSN: 0165-4608

LA: ENGLISH

AB: A case of acute nonlymphocytic leukemia after radiochemotherapy for Hodgkin's disease, with a rearrangement of 6p23 region, is described. This chromosome change, which has been previously reported in secondary leukemias or myelodysplastic syndromes, was an isolated karyotypic anomaly in our case, which strongly supports the nonrandom involvement of chromosome 6p in induced leukemias.

AN: 92274350

MEDLINE EXPRESS (R) 1991 - 1995 173 of 324

TI: Metabolic activation of sennoside C in mice: synergistic action of anthrones.

AU: Yamauchi-K; Shinano-K; Nakajima-K; Yagi-T; Kuwano-S

AD: Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan.

SO: J-Pharm-Pharmacol. 1992 Dec; 44(12): 973-6

This title is owned by this library

ISSN: 0022-3573

LA: ENGLISH

AB: Sennosides A and C directly injected into the caecum of mice showed equal purgative activity. Intracaecal administration reduced time to onset of diarrhoea induced by sennoside C from about 3 h after oral administration to about 24 min. At 2.3 h after oral administration of sennoside C, nearly equimolar amounts of aloe-emodin anthrone and rhein anthrone were detected in the large intestine of mice. The purgative effect of oral sennoside C could be reduced by pretreating mice with chloramphenicol. This was observed as a decreased formation of total anthrones in the large intestine. Both anthrones and an equimolar mixture of both anthrones directly injected into the caecum exerted a purgative effect, although the activity was lower for aloe-emodin anthrone. The intracaecal ED50 values were 54.5 (24.1-89.6), 11.4 (5.0-15.7) and 11.2 (6.1-14.6) mumol kg-1 for aloe-emodin anthrone, rhein anthrone and an equimolar mixture of both anthrones, respectively. We concluded that aloe-emodin anthrone and rhein anthrone, formed mainly by intraluminal bacterial action, are the true active metabolites of sennoside C in mice and that both anthrones synergistically exert their purgative effects on mice.

AN: 93108236

MEDLINE EXPRESS (R) 1991 - 1995 174 of 324

TI: Some pharmacological actions of aloe extracts and Cassia abbreviata on rats and mice.

AU: Parry-O; Matambo-C

AD: Department of Physiology, University of Zimbabwe, Harare.

SO: Cent-Afr-J-Med. 1992 Oct; 38(10): 409-14

ISSN: 0008-9176

LA: ENGLISH

AB: The effects of crude extracts (500 mg/kg IP and 500-1000 mg/kg PO) of three species of aloes and Cassia abbreviata in rats and mice were studied, particularly as regards their abortifacient actions. The LD50 values were as follows: A. globuligemma < 250 mg/kg IP; A.chabaudii 250-500 mg/kg IP; A. cryptopoda > 1500 mg/kg IP and C.abbreviata 500-750 mg/kg IP. Thus the most toxic was A.globuligemma. Their most visibly striking toxic effects in rats were CNS depression, and post mortem investigations showed widespread haemorrhagic lesions. Administration of the aloes and A.abbreviata to pregnant mice and rats did not cause expulsion or resorption of the foetuses. Several rats died within 36 hours of injection of A.chabaudii and A.globuligemma, but even in these rats there was no expulsion or resorption of the foetuses. Rats which survived the treatment delivered normal sized, healthy litters at term. It is suggested that neither the aloe species test nor C.abbreviata possess abortifacient activity at doses which are not toxic to the animals.

AN: 93380002

MEDLINE EXPRESS (R) 1991 - 1995 175 of 324

TI: In vivo activated brain astrocytes may produce and secrete nerve growth factor-like molecules.

AU: Oderfeld-Nowak-B; Bacia-A; Gradkowska-M; Fusco-M; Vantini-G; Leon-A; Aloe-L

AD: Nencki Institute of Experimental Biology, Department of Neurophysiology, Warsaw, Poland.

SO: Neurochem-Int. 1992 Oct; 21(3): 455-61

This title is owned by this library

ISSN: 0197-0186

LA: ENGLISH

AB: The cellular localization of the nerve growth factor-like immunoreactivity (NGF-LIR) has been studied in the septum and hippocampus of the rat brain 7 days following partial electrolytic lesion (2 mA, 30 s) of the septohippocampal pathways or after single intraventricular administration of 15 U of interleukin-1 beta (IL-1 beta). A double immunostaining technique which allowed a simultaneous localization of NGF-LIR and that of astroglia marker glial fibrillary acidic protein was used. Our data show that after both treatments, apart from neuronal localization of NGF-LIR typical for normal brain, many astrocytes both in the septum and hippocampus became NGF-like immunoreactive. Besides, NGF-LIR often formed a "halo" reaction around astrocytes. These results support the notion that activated in vivo brain astrocytes may, just as astrocytes growing in vitro, synthesize and secrete NGF-like molecules. Our findings may be of importance in considerations concerning trophic support to the cholinergic neurons of the basal forebrain nuclei whose impaired function is essentially responsible for some cognitive deficits in neurodegenerative diseases such as Alzheimer disease.

AN: 93264940

MEDLINE EXPRESS (R) 1991 - 1995 176 of 324

TI: Levocarnitine acetyl treatment promotes iris reinnervation following 6-hydroxydopamine-induced noradrenergic denervation in rats.

AU: De-Simone-R; Aloe-L; Ferraris-L; Ramacci-MT

AD: Institute of Neurobiology, National Research Centre, Rome, Italy.

SO: Int-J-Clin-Pharmacol-Res. 1992; 12(5-6): 281-7

ISSN: 0251-1649

LA: ENGLISH

AB: The effect of levocarnitine acetyl on neurite regeneration was evaluated following administration of 6-hydroxydopamine which caused a selective degeneration of peripheral catecholaminergic neurites in the irides of newborn and young rats. Levocarnitine acetyl 20 mg/kg body weight was administered either subcutaneously to newborn rats for 20 days or dissolved in drinking water to young rats for 90 days. Treatment with levocarnitine acetyl promoted neurite outgrowth following axotomy in both groups, thus demonstrating a stimulatory role of the substance in nerve regeneration of the peripheral nervous system. The results further confirm that levocarnitine acetyl is endowed with therapeutical efficacy in peripheral neuropathies.

AN: 93246328

MEDLINE EXPRESS (R) 1991 - 1995 177 of 324

TI: Studies on carrageenan-induced arthritis in adult rats: presence of nerve growth factor and role of sympathetic innervation.

AU: Aloe-L; Tuveri-MA; Levi-Montalcini-R

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: Rheumatol-Int. 1992; 12(5): 213-6

This title is owned by this library

ISSN: 0172-8172

LA: ENGLISH

AB: Recent studies effected by our Institute indicate that various forms of human arthritis express both immunohistochemically and biologically active nerve growth factor (NGF) in the synovium. In the present study, we used a model of carrageenan-induced arthritis to further evaluate the effects of joint inflammation on NGF level. These studies showed that experimentally-induced arthritis in rats caused a significant increase in NGF in the perivascular area of the synovium. We also showed that injection into the synovium of purified NGF did not cause inflammation per se and that the destruction of peripheral sympathetic innervation significantly reduced both the inflammation and the level of NGF following carrageenan injection.

AN: 93174145

MEDLINE EXPRESS (R) 1991 - 1995 178 of 324

TI: Multiple sclerosis patients express increased levels of beta-nerve growth factor in cerebrospinal fluid.

AU: Laudiero-LB; Aloe-L; Levi-Montalcini-R; Buttinelli-C; Schilter-D; Gillessen-S; Otten-U

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: Neurosci-Lett. 1992 Nov 23; 147(1): 9-12

This title is owned by this library

ISSN: 0304-3940

LA: ENGLISH

AB: We describe the measurement of beta-nerve growth factor (NGF) content in cerebrospinal fluid (CSF) from multiple sclerosis (MS) patients compared with CSF from age-matched normal subjects using a specific sandwich immunoassay (ELISA). During acute attacks patients exhibit a significant increase of NGF content compared to controls. In contrast during remission the mean NGF levels in CSF markedly decrease. These results strongly indicate that increased NGF production in CSF is a characteristic feature of the MS inflammatory response.

AN: 93125859

MEDLINE EXPRESS (R) 1991 - 1995 179 of 324

TI: In vivo and in vitro NGF studies on developing cerebellar cells.

AU: Aloe-L; Vigneti-E

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: Neuroreport. 1992 Mar; 3(3): 279-82

ISSN: 0959-4965

LA: ENGLISH

AB: The biological effect of nerve growth factor (NGF) in the early prenatal cerebellar cell was studied in vivo and in vitro with autoradiographic, tissue culture and immunohistochemical techniques. Iodinated NGF (125I-NGF) injected into the cerebella of 16-day-old rat embryos showed accumulation of this ligand in the Purkinje cell layers. The ability of these cells to accumulate NGF lasted to the 19th day of embryonic life. Cerebellar cells isolated from embryos of the same age, but not older embryos, cultured in vitro for two weeks in the presence of NGF, showed morphological characteristics similar to Purkinje-like cells. These findings suggest that NGF exerts a time-limited trophic effect on immature Purkinje cell precursors.

AN: 92385729

MEDLINE EXPRESS (R) 1991 - 1995 180 of 324

TI: Subchronic oral administration of acemannan in the rat and dog.

AU: Fogleman-RW; Shellenberger-TE; Balmer-MF; Carpenter-RH; McAnalley-BH

AD: Carrington Laboratories, Dallas, TX 75356.

SO: Vet-Hum-Toxicol. 1992 Apr; 34(2): 144-7

ISSN: 0145-6296

LA: ENGLISH

AB: Acemannan is the USAN-accepted name for long-chain polydispersed beta-(1,4)-acetylated polymannose with interspersed O-acetyl groups, with a mannose monomer/acetyl ratio of approximately 1:1. This complex polysaccharide is extracted from Aloe vera (barbadensis Miller); the technical material contains approximately 78% acemannan. Technical grade acemannan was administered po to rats for 14 d at 5% of the diet and for 6 mo at up to 2,000 mg/kg/d, and to beagle dogs for 90 d at up to 1,500 mg/kg/d without significant effect on any parameter measured in either species.

AN: 92377179

MEDLINE EXPRESS (R) 1991 - 1995 181 of 324

TI: [The clinical evaluation of the hypocholesterolemic effects of an inhibitor of cholesterol synthesis: mevalonic acid]

TO: Valutazione clinica degli effetti ipocolesterolemizzanti di un inibitore della sintesi del colesterolo: l'acido mevalonico.

AU: Del-Nero-E; Aloe-N; Augeri-C; Avola-F; Carta-G; Cavagnaro-A; De-Grandi-R; Gianfreda-M; Magro-GP; Mazzarello-GP; et-al

AD: I.N.R.C.A. sede di Genova.

SO: Clin-Ter. 1992 Jul; 141(7): 47-50

This title is owned by this library

ISSN: 0009-9074

LA: ITALIAN; NON-ENGLISH

AB: Twenty eight patients with heterozygous familial hypercholesterolemia were treated with mevalonic acid (an inhibitor of cholesterol synthesis) for 45 days. Patients received a daily dose of 750 to 1500 mg mevalonic acid depending on plasma cholesterol levels. Results showed a significant reduction in cholesterol values whereas no significant difference was observed in HDL cholesterol and triglyceride levels.

AN: 92370933

MEDLINE EXPRESS (R) 1991 - 1995 182 of 324

TI: Nerve growth factor and distribution of mast cells in the synovium of adult rats [letter]

AU: Aloe-L; Tuveri-MA; Levi-Montalcini-R

SO: Clin-Exp-Rheumatol. 1992 Mar-Apr; 10(2): 203-4

This title is owned by this library

ISSN: 0392-856X

LA: ENGLISH

AN: 92370835

MEDLINE EXPRESS (R) 1991 - 1995 183 of 324

TI: Ph-negative and bcr-negative atypical chronic myelogenous leukemia: biological features and clinical outcome.

AU: Montefusco-E; Alimena-G; Lo-Coco-F; De-Cuia-MR; Wang-YZ; Aloe-Spiriti-MA; Mancini-F; Cedrone-M; Mancini-M; Mandelli-F

AD: Department of Human Biopathology, University La Sapienza, Rome, Italy.

SO: Ann-Hematol. 1992 Jul; 65(1): 17-21

This title is owned by this library

ISSN: 0939-5555

LA: ENGLISH

AB: We report the clinical, hematologic, cytogenetic, and molecular characteristics of 13 patients with Philadelphia-negative (Ph-), bcr-negative atypical chronic myelogenous leukemia (CML). In the majority of cases, the phenotypic features at presentation resembled those of typical CML. However, these patients presented with a higher median age, lower median hemoglobin levels, and lower leukocyte and platelet counts than patients with Ph-positive CML. Cytogenetic analysis showed an abnormal karyotype in only one case. Southern blot investigation, using probes exploring the entire M-bcr region, demonstrated the absence of genomic bcr-abl rearrangements. The assessment of clonality in five patients (study of X-methylation patterns in females heterozygous at the DXS255 locus) indicated the proliferation of a monoclonal cell population. Disease evolution was mostly characterized by bone marrow failure, extramedullary infiltrates, and poor response to chemotherapy, without evidence of overt acute transformation. Our observations suggest that some hematologic and clinical features and the modalities of disease progression are presently the most helpful factors in distinguishing these bcr/abl-negative patients from those with typical bcr+CML. The differences existing also with chronic myelomonocytic leukemia (CMMoL), allow the consideration of ph-/bcr- CML as a separate entity, the nature of which remains to be elucidated.

AN: 92353211

MEDLINE EXPRESS (R) 1991 - 1995 184 of 324

TI: Cellular localization of nerve growth factor-like immunoreactivity in hippocampus and septum of adult rat brain.

AU: Bacia-A; Aloe-L; Fusco-M; Vantini-G; Leon-A; Oderfeld-Nowak-B

AD: Nencki Institute of Experimental Biology, Warsaw, Poland.

SO: Acta-Neurobiol-Exp-Warsz. 1992; 52(1): 1-7

This title is owned by this library

ISSN: 0065-1400

LA: ENGLISH

AB: The cellular localization of the nerve growth factor-like immunoreactivity (NGF-LIR) has been studied in the intact adult rat brain at the level of the hippocampus and the septum. Immunolabelling for NGF combined with counterstaining with cresyl violet and double immunostaining technique, which allowed simultaneous localization of NGF-LIR and that of astroglial marker -GFAP, were used. The data indicate neuronal localization of NGF-like immunoreactivity and a lack of colocalization of NGF-LIR with the immunoreactivity of GFAP in the hippocampus. These data are consistent with in situ hybridization results for NGF and immunocytochemical results for pro-NGF localization obtained by others. At the septal level, apart from neuronal localization of NGF-LIR, single NGF-like immunoreactive astrocytes have been observed. This suggests that, although to a very small extent, in vivo intact brain astrocytes may, just as astrocytes growing in vitro, synthesize NGF-like molecules. This finding may be of importance in better understanding the trophic support for NGF responsive cholinergic neurones in the brain.

AN: 92336843

MEDLINE EXPRESS (R) 1991 - 1995 185 of 324

TI: Short-term triazolam use improves nocturnal sleep of narcoleptics.

AU: Thorpy-MJ; Snyder-M; Aloe-FS; Ledereich-PS; Starz-KE

AD: Department of Neurology, Montefiore Medical Center, Bronx, NY 10467.

SO: Sleep. 1992 Jun; 15(3): 212-6

This title is owned by this library

ISSN: 0161-8105

LA: ENGLISH

AB: This study was undertaken to determine whether the use of triazolam by narcoleptic patients leads to improvement of nighttime sleep or excessive sleepiness. Ten narcoleptic patients, 5 males and 5 females, with complaints of sleep disturbance and aged between 18 and 60 years, were assigned to a single-blind within-subject crossover-designed study comparing placebo with 0.25 mg triazolam. All subjects completed sleep questionnaires and underwent 6 nights of polysomnographic testing. Following an adaptation night, subjects received either triazolam or placebo for 2 nights. Objective tests of sleepiness (multiple sleep latency testing/maintenance of wakefulness test) were performed. Sleep efficiency and overall sleep quality were improved on all triazolam nights. Daytime excessive sleepiness was not reduced objectively after triazolam. This study demonstrates that the short-term use of triazolam improves nocturnal sleep quality in narcoleptics. Studies of long-term administration of triazolam are required to determine if the improvement of nocturnal sleep is maintained.

AN: 92320170

MEDLINE EXPRESS (R) 1991 - 1995 186 of 324

TI: [Randomized study on the use of propofol for anesthesia in intracranial tumors. Comparison with balanced anesthesia]

TO: Studio randomizzato sull'uso del propofol nell'anestesia dei tumori intracranici. Confronto con anestesia bilanciata.

AU: Mazzitelli-MR; Morace-E; Sofra-M; Aloe-L; Moricca-P; Margiotta-L; Imperatori-G

AD: Servizio di Anestesia e Camere Operatorie, Istituto Regina Elena, Roma.

SO: Minerva-Anestesiol. 1992 Apr; 58(4 Suppl 1): 121-5

This title is owned by this library

ISSN: 0375-9393

LA: ITALIAN; NON-ENGLISH

AB: There is currently no anesthesiological technique which enables volume, cerebral blood flow and spinal fluid pressure to be kept constant. The aim of the present study was to compare two techniques of anesthesia in patients with cerebral tumours: one of a balanced type (AB) and the other of a continuous ev type (AEC) using propofol in order to assess which guaranteed the greatest cerebral protection. The results of the study have shown that the use of propofol may represent a useful alternative in intracranial tumour surgery since changes in systolic pressure and heart rate observed during AEC anesthesia were statistically lower in comparison to AB anesthesia. No statistically significant differences were observed in relation to changes in diastolic pressure, PVC, ApCO2 and the incidence of bleeding or cerebral edema. The quality of awakening was better in patients in the AEC group in terms of renewed breathing and spontaneous motility, verbal response and space-time orientation.

AN: 92319385

MEDLINE EXPRESS (R) 1991 - 1995 187 of 324

TI: Toxicologic evaluation of injectable acemannan in the mouse, rat and dog.

AU: Fogleman-RW; Chapdelaine-JM; Carpenter-RH; McAnalley-BH

AD: Pharmakon Research International, Waverly, PA 18471.

SO: Vet-Hum-Toxicol. 1992 Jun; 34(3): 201-5

ISSN: 0145-6296

LA: ENGLISH

AB: Acemannan, the USAN-accepted name for long-chain polydispersed beta-(1,4)-acetylated polymannose with interspersed 0-acetyl groups with a mannose monomer/acetyl ratio of approximately 1:1 and extracted from Aloe vera (barbadensis Miller), was administered as a 1.0 mg/ml solution to mice, rats and dogs, either as single dose or repeated at 4-d intervals for 8 doses by iv or ip routes. No significant signs of intoxication and no deaths occurred in animals treated with the single injection of acemannan at dosages of 80 mg/kg iv or 200 mg/kg ip in mice, 15 mg/kg iv or 50 mg/kg ip in rats, and 10 mg/kg iv or 50 mg/kg ip in dogs. On repeated injections systemic toxicity was limited to obvious transient discomfort that appeared dose related. There was accumulation of macrophages and monocytes without subsequent inflammatory reaction in lungs of the iv-treated animals, and in liver and spleen and on peritoneal surfaces of ip-treated animals. The effects were not considered adverse, but were consistent with the known immune stimulating activity of acemannan. A few deaths occurred in mice and rats that were suggestive of resulting from improper injection or sequella of necrosis of the injection site. The NOAELs for acemannan determined from these repeated injection studies were 20 mg/kg iv or ip in the mouse, 4.0 mg/kg iv and 50 mg/kg ip in the rat, and 1.0 mg/kg iv in dogs; 5.0 mg acemannan/kg ip in the dog was considered to be LOAEL, based on the emesis and abdominal discomfort induced.

AN: 92303099

MEDLINE EXPRESS (R) 1991 - 1995 188 of 324

TI: Photodermatitis to aloe vera [letter; comment]

AU: Dominguez-Soto-L

SO: Int-J-Dermatol. 1992 May; 31(5): 372

This title is owned by this library

ISSN: 0011-9059

LA: ENGLISH

AN: 92267774

MEDLINE EXPRESS (R) 1991 - 1995 189 of 324

TI: Aloe vera and the inflamed synovial pouch model.

AU: Davis-RH; Stewart-GJ; Bregman-PJ

AD: Pennsylvania College of Podiatric Medicine, Philadelphia.

SO: J-Am-Podiatr-Med-Assoc. 1992 Mar; 82(3): 140-8

ISSN: 8750-7315

LA: ENGLISH

AB: Administration of air under the skin produced a pouch wall that closely resembled a synovium in that the inner lining was made up of macrophages and fibroblasts. Administration of 1% carrageenan directly into the 7-day-old air pouch produced an inflammation characterized by an increased number of mast cells in pouch fluid as well as an increase in wall vascularity. A punch biopsy weight of the pouch wall did not reveal an increase in 1% carrageenan-treated animals. However, a 10% Aloe vera treatment of carrageenan-inflamed synovial pouches reduced the vascularity 50% and the number of mast cells in synovial fluid 48%. The pouch wall punch biopsy weight was increased by A. vera, which was verified by histologic examination of the inner synovial lining. Aloe vera stimulated the synovial-like membrane, as evidenced by an increased number of fibroblasts, suggesting that A. vera stimulated fibroblasts for growth and repair of the synovial model. The synovial air pouch can be used to study simultaneously the acute anti-inflammatory and fibroblast stimulating activities of A. vera.

AN: 92251606

MEDLINE EXPRESS (R) 1991 - 1995 190 of 324

TI: NGF decreases isolation-induced aggressive behavior, while increasing adrenal volume, in adult male mice.

AU: Bigi-S; Maestripieri-D; Aloe-L; Alleva-E

AD: Section of Behavioral Pathophysiology, Istituto Superiore di Sanita, Rome, Italy.

SO: Physiol-Behav. 1992 Feb; 51(2): 337-43

This title is owned by this library

ISSN: 0031-9384

LA: ENGLISH

AB: Intravenous administration of highly purified murine nerve growth factor (NGF, either 15 or 30 micrograms/subject/day) for six consecutive days to adult male mice of the CD-1 strain markedly influenced a number of items of aggressive behavior induced by 5 weeks of individual housing. Control mice received the same doses of cytochrome c. During a 20-min fighting session on day 7, both NGF-treated groups showed longer Latency to the first Attack, while Total Attacking Time and Aggressive grooming were significantly lower when compared to control animals. Twenty-four h later, adrenals from the NGF- and cytochrome c-treated groups were dissected and analyzed by means of a Vidas Image-Analyzer. NGF administration produced a remarkable dose-dependent increase in both adrenal weight and volume (particularly in the medullary zone), supporting the hypothesis that adrenals represent a possible target for the NGF release from submaxillary salivary glands occurring upon intraspecific fighting.

AN: 92213111

MEDLINE EXPRESS (R) 1991 - 1995 191 of 324

TI: Nerve growth factor in the synovial fluid of patients with chronic arthritis.

AU: Aloe-L; Tuveri-MA; Carcassi-U; Levi-Montalcini-R

AD: Institute of Neurobiology, Consiglio Nazionale delle Ricerche, Rome, Italy.

SO: Arthritis-Rheum. 1992 Mar; 35(3): 351-5

This title is owned by this library

ISSN: 0004-3591

LA: ENGLISH

AB: Cytokines regulate nerve growth factor (NGF) synthesis during inflammatory processes. Since cytokines are also involved in the inflammatory processes of autoimmune rheumatic diseases, we examined levels of NGF in patients with rheumatoid or other types of chronic arthritis. NGF was present in the synovial fluid and synovium of patients with chronic arthritis, but was undetectable in control fluids. We conclude that NGF might be involved in the pathogenesis of arthritis.

AN: 92162082

MEDLINE EXPRESS (R) 1991 - 1995 192 of 324

TI: The effect of a plants mixture extract on liver gluconeogenesis in streptozotocin induced diabetic rats.

AU: al-Awadi-F; Fatania-H; Shamte-U

AD: Department of Biochemistry, Faculty of Medicine, Kuwait University, Safat.

SO: Diabetes-Res. 1991 Dec; 18(4): 163-8

This title is owned by this library

ISSN: 0265-5985

LA: ENGLISH

AB: We have previously reported on plant mixture extract comprising of Nigella sativa, Myrrh, Gum Olibanum, Gum Asafoetida and Aloe to have a blood glucose lowering effect. The present study with streptozotocin diabetic rats is focussed on the mechanism of action, specifically on a) hepatic gluconeogenesis b) activity of key gluconeogenic enzymes, pyruvate carboxylase (PC) and phosphoenol-pyruvate carboxykinase (PEPCK). Similar studies using a biguanide, phenformin, have been conducted to compare the mode of action of these two compounds. The blood glucose levels (mean +/- SEM) before and after treatment with the plants extract were (16.7 +/- 1.7 mmol/L and 8.5 +/- 1.3 mmol/L) and with phenformin (15.1 +/- 1.3 mmol/L and 10.7 +/- 1.5 mmol/L). The rate of gluconeogenesis in isolated hepatocytes as well as activity of PC and PEPCK in liver homogenates is significantly lowered following treatment with the plants extract. Although phenformin also lowers blood glucose, it does not affect hepatic gluconeogenesis under stated experimental conditions. It is concluded that the anti-diabetic action of the plants extract may, at least partly, be mediated through decreased hepatic gluconeogenesis. The extract may prove to be a useful therapeutic agent in the treatment of non-insulin dependent diabetes mellitus (NIDDM).

AN: 93105582

MEDLINE EXPRESS (R) 1991 - 1995 193 of 324

TI: Isolation of a stimulatory system in an Aloe extract.

AU: Davis-RH; Parker-WL; Samson-RT; Murdoch-DP

AD: Pennsylvania College of Podiatric Medicine, Philadelphia 19107.

SO: J-Am-Podiatr-Med-Assoc. 1991 Sep; 81(9): 473-8

ISSN: 8750-7315

LA: ENGLISH

AB: The authors' previous work on a 50% ethanol extract of Aloe vera was done to evaluate anti-inflammatory activity using the croton oil-induced ear swelling assay. The anti-inflammatory activity was found in the supernatant fraction. The supernatant fraction decreased inflammation, when applied topically, by 29.2%, and the precipitate decreased inflammation by 12.1%. However, in the present work, the precipitate fraction decreased the wound diameter by an average of 47.1% (stimulatory system). Little or no wound healing activity was found in the supernatant. Aloe vera appears to act as a modulatory system toward wounds and inflammation and is a potentially valuable tool for managing lower extremity conditions.

AN: 92085227

MEDLINE EXPRESS (R) 1991 - 1995 194 of 324

TI: Effect of acetyl-L-carnitine on forebrain cholinergic neurons of developing rats.

AU: De-Simone-R; Ramacci-MT; Aloe-L

AD: Institute of Neurobiology, C.N.R., Rome, Italy.

SO: Int-J-Dev-Neurosci. 1991; 9(1): 39-46

This title is owned by this library

ISSN: 0736-5748

LA: ENGLISH

AB: It has been shown that the endogenous compound, acetyl-L-carnitine (ALCAR), acts in the brain as a metabolic cofactor in the synthesis of acetylcholine. In these studies, ALCAR was injected into the brain of developing rats every other day for the first three weeks after birth in order to assess its effect on forebrain cholinergic neurons. The results showed that intracerebroventricular (icv) administration of ALCAR causes an increase of choline acetyltransferase (ChAT) activity and of nerve growth factor receptor expression in the striatum. Biological assays of brain tissues revealed that the level of nerve growth factor (NGF) in the hippocampus also increases. The ability of brain cholinergic tissues to respond to exogenous administration of ALCAR is discussed.

AN: 91196618

MEDLINE EXPRESS (R) 1991 - 1995 195 of 324

TI: In vitro virucidal activity of selected anthraquinones and anthraquinone derivatives.

AU: Andersen-DO; Weber-ND; Wood-SG; Hughes-BG; Murray-BK; North-JA

AD: Department of Microbiology, Brigham Young University, Provo, Utah 84602.

SO: Antiviral-Res. 1991 Sep; 16(2): 185-96

ISSN: 0166-3542

LA: ENGLISH

AB: Anthraquinones and anthraquinone derivatives were characterized for their antiviral and virucidal activities against viruses representing several taxonomic groups. One of these compounds, hypericin, had activity against vesicular stomatitis virus, herpes simplex virus types 1 and 2, parainfluenza virus, and vaccinia virus (from 0.5 to 3.8 log10 reductions in infectivity) at concentrations of less than 1 microgram/ml as determined by a direct pre-infection incubation assay. Human rhinovirus was not sensitive to hypericin at concentrations up to 10 micrograms/ml. Addition of small amounts of Tween-80 to solutions containing hypericin enhanced, by up to 2.6 log10, hypericin's virucidal activity. Anthraquinones and anthraquinone derivatives with the hydroxyl and alkyl substitution pattern of emodin (i.e. emodin, emodin anthrone, emodin bianthrone and hypericin) were active against the enveloped viruses tested. The following general pattern of activity was found: hypericin greater than emodin bianthrone greater than emodin anthrone greater than emodin. Chrysophanic acid, aloe-emodin, and sennosides A and B did not possess activity against any of the viruses tested.

AN: 92189336

MEDLINE EXPRESS (R) 1991 - 1995 196 of 324

TI: [Mechanochemical solid state reactions of natural products for medicinal use containing hydroxyanthraquinone derivatives]

AU: Kuzuya-M; Sakata-H; Kondo-S; Noguchi-A

AD: Gifu Pharmaceutical University, Japan.

SO: Yakugaku-Zasshi. 1991 Nov; 111(11): 665-71

ISSN: 0031-6903

LA: JAPANESE; NON-ENGLISH

AB: In commercial powdered natural products for medicinal use containing various combined forms of hydroxyanthraquinone derivatives such as Sennae Folium, Cassiae Semen, Rhei Rhizoma and Aloe a considerable amount of stable free radicals (ca. 10(17)-10(18) spin/g) was found to be contained by use of electron spin resonance (ESR) spectral measurements. It was also found that the vibratory milling of such powders in a metallic vessel enhanced the ESR spectral intensities, demonstrating the occurrence of mechanoradical formation. Separate experiments also demonstrated that the vibratory milling of various kinds of powdered hydroxyanthraquinone derivatives mixed with calcium oxalate has produced the mechanoradicals effectively, but they decayed gradually on standing at room temperature. It was suggested, therefore, that the mechanoradicals formed in the above natural products are metal complexes of the corresponding semiquinone anion radicals induced by solid state one electron transfer mechanism from the active metal surface, part of which is further immobilized in polymeric fibers or the like in the plant tissues.

AN: 92148655

MEDLINE EXPRESS (R) 1991 - 1995 197 of 324

TI: Intracerebral implantation of nerve growth factor-producing fibroblasts protects striatum against neurotoxic levels of excitatory amino acids.

AU: Schumacher-JM; Short-MP; Hyman-BT; Breakefield-XO; Isacson-O

AD: Neuroregeneration Laboratory, Harvard Medical School, Belmont, MA.

SO: Neuroscience. 1991; 45(3): 561-70

This title is owned by this library

ISSN: 0306-4522

LA: ENGLISH

AB: With the exception of L-DOPA pharmacological treatment in Parkinson's disease, the neurodegenerative diseases lack effective treatment. Previous studies of neurodegenerative diseases suggest that symptoms arise secondary to defects in local neuronal circuitry and cannot be treated effectively with systemic drug delivery. Therefore, a promising treatment is the application of fetal or genetically engineering cells which protect or replace neurons in deficient regions. Engineered cells can be derived from cell lines or grown from recipient host fibroblasts or other cells, then modified to produce and secrete substances at a specific area of the brain. A previous study using parallel intracerebral infusions of nerve growth factor and an excitotoxic amino acid into the rat striatum demonstrated a protective effect of nerve growth factor on neurons [Aloe L. (1987) Biotechnology 5, 1085-1086]. In order to further test this paradigm, we have utilized a biological delivery system of nerve growth factor by implanting fibroblasts into the rat striatum which secrete high levels of nerve growth factor, prior to infusing the neurotoxins quinolinate or quisqualate. Animals in this group had smaller lesions than did a group implanted with a similar non-nerve growth factor-producing graft. In addition, marked neuronal sparing was noted within areas of lesions in those animals containing a nerve growth factor-producing graft. These results indicate that implantation of genetically engineered nerve growth factor-secreting cells can be used to protect neurons at a specific target from excitotoxin-induced lesions.

AN: 92131247

MEDLINE EXPRESS (R) 1991 - 1995 198 of 324

TI: Aloe vera. Its chemical and therapeutic properties [see comments]

AU: Shelton-RM

AD: Department of Dermatology, Brooke Army Medical Center, Fort Sam Houston, San Antonio, Texas.

SO: Int-J-Dermatol. 1991 Oct; 30(10): 679-83

This title is owned by this library

ISSN: 0011-9059

LA: ENGLISH

AN: 92064333

MEDLINE EXPRESS (R) 1991 - 1995 199 of 324

TI: Experimental study of the hypoglycemic effect of some antidiabetic plants.

AU: Roman-Ramos-R; Flores-Saenz-JL; Partida-Hernandez-G; Lara-Lemus-A; Alarcon-Aguilar-F

AD: Health Sciences Department, Metropolitan Autonomous University, Iztapalapa Campus, Mexico, D.F.

SO: Arch-Invest-Med-Mex. 1991 Jan-Mar; 22(1): 87-93

This title is owned by this library

ISSN: 0066-6769

LA: ENGLISH

AB: The purpose of this work is to look for the hypoglycemic effect of 12 plants most used in Mexico for controlling diabetes mellitus. The studies were realized in 27 rabbits submitted weekly to glucose tolerance tests after gastric administration of water, tolbutamide or a preparation of the plant. The results showed that tolbutamide and studied plants (except Aloe barbadensis) decreased significantly (p less than 0.05) the area under glucose tolerance curve, in relation with the water control. The strongest effect was yielded by Psacalium peltatum (27.9%), followed by Curcubita ficifolia (26.4%), Lepechinia caulescens (26.0%), Opuntia streptacantha (21.4%), Slanum verbascifolum (21.1%), Teucrium cubense (19.4%), Cecropia obtusifolia (18.9%), Phaseolus vulgaris (18.5%), Tecoma stans (17.5%), Eriobotrya japonica (17.2%), Salpianthus macrodonthus (15.0%), tolbutamide (14.3%), and Aloe barbadensis (1.4%). Our results point out that the majority of the plants most used by the Mexican population to control diabetes mellitus have an evident hypoglycemic action.

AN: 92313168

MEDLINE EXPRESS (R) 1991 - 1995 200 of 324

TI: Inactivation of enveloped viruses by anthraquinones extracted from plants.

AU: Sydiskis-RJ; Owen-DG; Lohr-JL; Rosler-KH; Blomster-RN

AD: Department of Microbiology, University of Maryland, Baltimore 21201.

SO: Antimicrob-Agents-Chemother. 1991 Dec; 35(12): 2463-6

This title is owned by this library

ISSN: 0066-4804

LA: ENGLISH

AB: To determine the extent of antiviral activity present in a number of plant extracts, hot glycerin extracts were prepared from Rheum officinale, Aloe barbadensis, Rhamnus frangula, Rhamnus purshianus, and Cassia angustifolia and their virucidal effects were tested against herpes simplex virus type 1. All the plant extracts inactivated the virus. The active components in these plants were separated by thin-layer chromatography and identified as anthraquinones. A purified sample of aloe emodin was prepared from aloin, and its effects on the infectivity of herpes simplex virus type 1 and type 2, varicella-zoster virus, pseudorabies virus, influenza virus, adenovirus, and rhinovirus were tested by mixing virus with dilutions of aloe emodin for 15 min at 37 degrees C, immediately diluting the sample, and assaying the amount of infectious virus remaining in the sample. The results showed that aloe emodin inactivated all of the viruses tested except adenovirus and rhinovirus. Electron microscopic examination of anthraquinone-treated herpes simplex virus demonstrated that the envelopes were partially disrupted. These results show that anthraquinones extracted from a variety of plants are directly virucidal to enveloped viruses.

AN: 92238665

MEDLINE EXPRESS (R) 1991 - 1995 201 of 324

TI: Eating epilepsy.

AU: Haddad-MS; Puglia-Junior-JP; Navarro-JM; Gronich-G; Scapolan-HB; Aloe-FS; Vilela-MM; Naylor-FG; Cukiert-A; Mariano-Junior-R

AD: Department of Neurology, University of Sao Paulo Faculty of Medicine, Brazil.

SO: Arq-Neuropsiquiatr. 1991 Sep; 49(3): 326-9

This title is owned by this library

ISSN: 0004-282X

LA: ENGLISH

AB: Eating epilepsy is a rare type of reflex epilepsy. A 24 years-old male with eating reflex complex partial seizures was submitted to clinical, neurological, neuroradiological and EEG studies. Neurologic and CT examinations were normal. EEG recordings including video-EEG monitoring during meals disclosed focal abnormalities related to both temporal lobes prevailing at the left side and secondary bilateral synchrony mainly in more anterior regions. Ictal findings were similar to the interictal secondary bilateral synchrony except for its longer duration. PB, VPA and DPH monotherapies were ineffective. High dose CBZ monotherapy yielded good but incomplete seizure control. Since a big number of precipitants could be involved, no specific physiopathological basis could be established.

AN: 92222391

MEDLINE EXPRESS (R) 1991 - 1995 202 of 324

TI: [Cardiopulmonary echinococcosis: a report of a clinical case (see comments)]

TO: Echinococcosi cardio-polmonare: descrizione di un caso clinico.

AU: Papagna-D; Aloe-MA; Spagnolo-S

AD: Servizio di Cardiologia, Ospedale S. Martino, Genova.

SO: G-Ital-Cardiol. 1991 Oct; 21(10): 1129-33

This title is owned by this library

ISSN: 0046-5968

LA: ITALIAN; NON-ENGLISH

AB: The authors report a case of hydatid cyst of the ventricular septum. The patient, an asymptomatic 30-year-old woman, was hospitalized in the Department of Pneumology after a routine chest X-ray test which detected two round masses in the lungs. During hospitalization the patient was asymptomatic and standard ECG was normal, but two-dimensional echocardiography (2D echo) revealed a cardiac cyst localized in the mediobasal portion of the ventricular septum. The finding was confirmed by computed tomography and nuclear magnetic resonance imaging. An echinococcosis was suspected on the basis of the patient's origin (Morocco) and because of the presence of cystic lesion also in the lungs, and was confirmed by positive hemagglutinin test. The patient underwent surgery (suction of cardiac cyst in extracorporeal circulation and washing with formaline 2%). This case is of particular interest because of the rarity of cardiac localization of echinococcosis (0.02-2%) and the lack of symptoms or of asymptomatic cardiac arrhythmias despite the location in the ventricular septum. Patients with hepatic or pulmonary hydatid disease could be routinely investigated by 2D echo because this imaging technique is: noninvasive, feasible and has a high sensitivity for detecting intracardiac echinococcal cysts.

AN: 92209937

MEDLINE EXPRESS (R) 1991 - 1995 203 of 324

TI: A double-blind trial of a celandin, aloevera and psyllium laxative preparation in adult patients with constipation.

AU: Odes-HS; Madar-Z

AD: Intestinal Diseases Unit, Soroka Medical Center, Beer Sheva, Israel.

SO: Digestion. 1991; 49(2): 65-71

This title is owned by this library

ISSN: 0012-2823

LA: ENGLISH

AB: The aim of this study was to evaluate the effect of a novel laxative preparation, composed of celandin, aloevera and psyllium in patients with chronic constipation. Thirty-five men and women were randomized to receive capsules containing celandin-aloevera-psyllium, or placebo, in a double-blind trial lasting 28 days. Symptoms in the last 2 weeks of the treatment period were compared to those in the 14-day pre-trial basal period. In the celandin, aloevera and psyllium group, bowel movements became more frequent, the stools were softer and laxative dependence was reduced. In the placebo group, all these parameters were unchanged. Abdominal pain was not reduced in either group. The results of this study show that the preparation is an effective laxative in the treatment of constipation.

AN: 92192352

MEDLINE EXPRESS (R) 1991 - 1995 204 of 324

TI: [Isolation and identification of aloesin from the leaves of Aloe vera L. var, chinensis (Haw.) Berger]

AU: Yuan-A; Kang-S; Qin-L; Ruan-B; Fan-Y

AD: Guangxi Institute of Traditional Chinese Medical and Pharmaceutical Sciences, Nanning.

SO: Chung-Kuo-Chung-Yao-Tsa-Chih. 1991 May; 16(5): 292-3, 319

ISSN: 1001-5302

LA: CHINESE; NON-ENGLISH

AN: 92143949

MEDLINE EXPRESS (R) 1991 - 1995 205 of 324

TI: [Immunomodulation in patients with cavernous kidney tuberculosis using piperazine adipinate and amniocene]

TO: Immunokorrektsiia u bol'nykh kavernoznym tuberkulezom pochek piperazina adipinatom i amniotsenom.

AU: Kozin-IuI; Cherniavskii-VI

SO: Probl-Tuberk. 1991(6): 29-32

ISSN: 0032-9533

LA: RUSSIAN; NON-ENGLISH

AB: Findings of a complex immunologic examination of 138 patients with active cavernous tuberculosis of the kidneys concurrent with secondary immunodeficiency are presented. Biostimulators and immunomodulating drugs were administered as conservative treatment in this case. Changes in cellular and humoral immunity indices as well as those of the mononuclear-phagocyte system before and after treatment were traced in the groups of patients receiving biogenic stimulators (such, as aloe extract, vitreous body, plasmol, Fibs) and immunoregulating drugs (like piperazine adipinate and injected amniocene). It was found that the biogenic stimulators fail to have a pronounced effect on the immunologic indices. The inclusion of piperazine adipinate and injected amniocene into a complex of antibiotic and chemotherapy brings about a significant improvement of the cellular immunity indices and the mononuclear-phagocyte system function which are inhibited to a greater extent in patients with active destructive nephrophthisis. A recommended use of piperazine adipinate and amniocene as adjuncts to a complex treatment has been proved in patients of this category by means of clinicoimmunologic correlations.

AN: 92141160

MEDLINE EXPRESS (R) 1991 - 1995 206 of 324

TI: In vivo and in vitro effect of NGF on bursa of Fabricius cells during chick embryo development.

AU: Laudiero-LB; Vigneti-E; Aloe-L

AD: Institute of Neurobiology, C.N.R., Rome, Italy.

SO: Int-J-Neurosci. 1991 Jul; 59(1-3): 189-98

This title is owned by this library

ISSN: 0020-7454

LA: ENGLISH

AB: NGF exerts a broader biological action than previously believed. The growing-evidence of NGF's effect on lymphocytes and the presence of high levels of mRNA for NGF receptors in the embryonic bursa of Fabricius led us to study the action of NGF on bursal cells during chick embryo development. Our in vivo experiments indicate that NGF administration in ovo caused a significant increase in size of the lymphoid follicles in the embryonic bursa at E15. The in vitro experiments demonstrated that NGF exhibits different effects on bursal cells depending on the stage of development. At E9 bursal cells survive for longer periods when NGF is added to the medium. At E15 NGF act as a colony-stimulating factor by significantly increasing the number of colonies in soft agar cultures.

AN: 92129073

MEDLINE EXPRESS (R) 1991 - 1995 207 of 324

TI: Prophylactic administration of tranexamic acid preserves platelet numbers during extracorporeal circulation in rabbits.

AU: Plotz-FB; van-Oeveren-W; Aloe-LS; Riley-MK; Hultquist-KA; Bartlett-RH; Wildevuur-CR

AD: Department of Pediatrics, University Hospital, Groningen, The Netherlands.

SO: ASAIO-Trans. 1991 Jul-Sep; 37(3): M416-7

ISSN: 0889-7190

LA: ENGLISH

AB: Neonatal extracorporeal life support is associated with platelet consumption and hemostatic disorders. This study in rabbits was undertaken to evaluate the effect of heparin coating and of the plasminogen and plasmin inhibitor, tranexamic acid on platelet consumption during extracorporeal circulation. Fibrinogen consumption was prevented by heparin coating, but platelet consumption was only prevented after the prophylactic administration of tranexamic acid. The authors concluded that inhibition of the fibrinolytic system had a specific effect on preserving platelet numbers, rather than improving the thromboresistance of the artificial surface. The main advantage of a heparin coating is the anticoagulant activity of the surface. Thus systemic heparinization can be reduced or omitted.

AN: 92088726

MEDLINE EXPRESS (R) 1991 - 1995 208 of 324

TI: In vivo effect of granulocyte-macrophage colony-stimulating factor on the kinetics of human acute myeloid leukemia cells.

AU: Aglietta-M; De-Felice-L; Stacchini-A; Petti-MC; Bianchi-AC; Aloe-Spiriti-MA; Sanavio-F; Apra-F; Piacibello-W; Stern-AC; et-al

AD: Clinica Medica, Dipartimento di Scienze Biomediche ed Oncologia Umana, Universita di Torino, Italy.

SO: Leukemia. 1991 Nov; 5(11): 979-84

This title is owned by this library

ISSN: 0887-6924

LA: ENGLISH

AB: Granulocyte-macrophage colony-stimulating factor, (GM-CSF) was given at 8 micrograms/kg daily by continuous i.v. infusion for 72 h to six patients with acute myeloid leukemia (AML) in expansion and one with chronic myeloid leukemia in blastic crisis to determine whether it was possible to augment the proliferative activity of the neoplastic population. The percentage of marrow blasts in S phase (labeling index, LI) was increased in five patients (1.3-, 1.5-, 1.9-, 2.3- and 3.2-fold change). The increase in LI was similar 24 and 48 h after beginning GM-CSF. The RNA Index also increased in patients who showed an increased LI, suggesting that GM-CSF had recruited quiescent neoplastic cells into the cell cycle. Forty eight hours after beginning GM-CSF, chemotherapy was started. The fate of S phase cells, labeled in vivo with bromodeoxyuridine (BrdU) immediately before cytostatic treatment, was monitored. BrdU positive cells were identified by fluorescent antibody for up to 28 days. A preferential killing of BrdU (S phase) cells was observed in 5/7 patients who obtained a complete remission, whereas this was not apparent in the two patients who achieved only a partial remission. Chemotherapy induced a rapid and profound aplasia; its duration, however, was not significantly different from that observed in historical controls. GM-CSF may have a potential role in the treatment of AML, as this study shows that it recruits leukemic cells into the cell cycle without adversely prolonging aplasia after cycle-specific therapy.

AN: 92072366

MEDLINE EXPRESS (R) 1991 - 1995 209 of 324

TI: Thymopentin in the treatment of myelodysplastic syndromes [letter]

AU: Latagliata-R; Petti-MC; Aloe-Spiriti-MA; Tirindelli-MC; Fazi-P; De-Gregoris-C; Mandelli-F

SO: Acta-Haematol. 1991; 86(2): 115

This title is owned by this library

ISSN: 0001-5792

LA: ENGLISH

AN: 92057463

MEDLINE EXPRESS (R) 1991 - 1995 210 of 324

TI: Decreased mortality of Norman murine sarcoma in mice treated with the immunomodulator, Acemannan.

AU: Peng-SY; Norman-J; Curtin-G; Corrier-D; McDaniel-HR; Busbee-D

AD: Department of Anatomy, College of Veterinary Medicine, Texas A & M University, College Station 77843.

SO: Mol-Biother. 1991 Jun; 3(2): 79-87

ISSN: 0952-8172

LA: ENGLISH

AB: An extract from the parenchyma of Aloe barbadensis Miller shown to contain long chain polydispersed beta (1,4)-linked mannan polymers with random O-acetyl groups (acemannan, Carrisyn) was found to initiate the phagocyte production of monokines that supported antibody dependent cellular cytotoxicity and stimulated blastogenesis in thymocytes. Acemannan, in both enriched and highly purified forms, was administered intraperitoneally to female CFW mice into which murine sarcoma cells had been subcutaneously implanted. The rapidly growing, highly malignant and invasive sarcoma grew in 100% of implanted control animals, resulting in mortality in 20 to 46 days, dependent on the number of cells implanted. Approximately 40% of animals treated with acemannan at the time of tumor cell implantation (1.5 x 10(6) cells) survived. Tumors in acemannan-treated animals exhibited vascular congestion, edema, polymorphonuclear leukocyte infiltration, and central necrosing foci with hemorrhage and peripheral fibrosis. The data indicate that in vivo treatment of peritoneal macrophages stimulates the macrophage production of monokines, including interleukin-1 and tumor necrosis factor. The data further indicate that sarcomas in animals treated i.p. with acemannan at the time of tumor cell implantation were infiltrated by immune system cells, became necrotic, and regressed. The combined data suggest that acemannan-stimulated synthesis of monokines resulted in the initiation of immune attack, necrosis, and regression of implanted sarcomas in mice.

AN: 92000401

MEDLINE EXPRESS (R) 1991 - 1995 211 of 324

TI: [Studies on chemical protectors against radiation. XXXIII. Protective mechanisms of various compounds against skin injury induced by radiation]

AU: Sato-Y; Kumazawa-N; Suzuki-M; Wang-CM; Ohta-S; Shinoda-M

AD: Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan.

SO: Yakugaku-Zasshi. 1991 Jan; 111(1): 51-8

ISSN: 0031-6903

LA: JAPANESE; NON-ENGLISH

AB: The radiation protective mechanisms on skin injury induced by soft X-irradiation were investigated by use of various radiation protective agents such as sulfur compounds (MEA, MEG, thiourea), nucleic acid constitutional compounds (adenosine, inosine), antioxidative compounds (sesamol, ferulic acid, ascorbic acid), crude drugs (Rosae Fructus, Anemarrhenae Rhizoma, Trapae Fructus, Forsythiae Fructus, Aloe arborescens). Scavenge action of activated oxygen, inhibitory effect of lipid peroxidation, induction of antioxidative protein and protective effect against damage of deoxyribonucleic acid and superoxide dismutase by X-irradiation were evaluated as the radiation protective mechanisms, and relationship between these results and protective effect of skin injury induced by radiation was studied.

AN: 91277966

MEDLINE EXPRESS (R) 1991 - 1995 212 of 324

TI: Radical surgery in rectal cancer patients: what does it mean today?

AU: Cavaliere-R; Tedesco-M; Giannarelli-D; Aloe-L; Perri-P; Di-Filippo-F; Crecco-M; Gabrielli-F; Cosimelli-M; Stipa-S

AD: Regina Elena National Cancer Institute, Rome, Italy.

SO: J-Surg-Oncol-Suppl. 1991; 2: 24-31

This title is owned by this library

ISSN: 0022-4790

LA: ENGLISH

AN: 91369431

MEDLINE EXPRESS (R) 1991 - 1995 213 of 324

TI: Advanced colorectal cancer: impact of chemotherapy on survival.

AU: Cortesi-E; Padovani-A; Aloe-A; Picece-V; Pellegrini-P; Pellegrini-A

AD: Department of Experimental Medicine, University of Rome, La Sapienza, Italy.

SO: J-Surg-Oncol-Suppl. 1991; 2: 112-5

This title is owned by this library

ISSN: 0022-4790

LA: ENGLISH

AN: 91369417

MEDLINE EXPRESS (R) 1991 - 1995 214 of 324

TI: The use of botanicals for health purposes by members of a prepaid health plan.

AU: Brown-JS; Marcy-SA

AD: School of Nursing, Oregon Health Sciences University, Portland 97201.

SO: Res-Nurs-Health. 1991 Oct; 14(5): 339-50

ISSN: 0160-6891

LA: ENGLISH

AB: Interviews were conducted with 100 adults (27 men, 73 women) enrolled in a prepaid medical health plan to investigate their use of botanical remedies. They were asked which of 50 listed herbs they or members of their families had used for health purposes and with what effect; which of 60 listed health problems they had treated with home remedies; and what additional home remedies or alternative health care resources they had used. Over 100 different home remedies were identified, with most considered effective. Individual respondents used from 0 to 33 herbal and plant remedies (Md = 7), some of which have toxic properties. A remedy was reported for almost every health problem listed. Substances most frequently used were aloe vera, honey, peppermint, garlic, eucalyptus, and rose hips; health problems most frequently treated were burns, colds, indigestion, insect bites, insomnia, rashes. Persons who were married, from larger households, of higher socioeconomic status, who had consulted alternative healers, or who had patronized health food stores tended to use home remedies more than their counterparts. Implications for further evaluation of self-care practices are discussed.

AN: 91368053

MEDLINE EXPRESS (R) 1991 - 1995 215 of 324

TI: Capsaicin affects aggressive behavior, but not hot plate responding, of adult male mice.

AU: Alleva-E; Aloe-L; Bigi-S; De-Acetis-L

AD: Section of Behavioral Pathophysiology, Istituto Superiore di Sanita, Rome, Italy.

SO: Physiol-Behav. 1991 Apr; 49(4): 715-9

This title is owned by this library

ISSN: 0031-9384

LA: ENGLISH

AB: Adult male mice of albino Swiss-derived CD-1 strain were used to assess the effects of capsaicin (a powerful agent that produces a marked depletion of the undecapeptide substance P) on both intraspecific aggressive behavior (induced by 8 weeks of individual housing) and pain sensitivity. Capsaicin was given SC, 48 h before behavioral testing. Aggressive behavior, scored during a 5-min session under red light, was significantly enhanced by capsaicin treatment (50 or 100 microliters of a 7.5 mg/ml solution). In fact, Total Aggressive Episodes, Attacks, and Upright Offensive Posture were significantly higher in the two capsaicin-treated groups, while Latency to the first Attack was decreased, when compared to both vehicle or unhandled controls. A concomitant decrease in Submissive Postures and Flee was also evident in capsaicin mice. Hot plate testing (55 +/- 0.1 degrees C, cutoff time 30 s), carried out on nonisolated mice, did not reveal any difference among the two capsaicin groups (same doses) and vehicle or unhandled controls.

AN: 91352161

MEDLINE EXPRESS (R) 1991 - 1995 216 of 324

TI: Barbaloin stimulates growth of Eubacterium sp. strain BAR, a barbaloin-metabolizing bacterium from human feces.

AU: Che-QM; Akao-T; Hattori-M; Tsuda-Y; Namba-T; Kobashi-K

AD: Research Institute for Wakan-Yaku (Traditional Sino-Japanese Medicines), Toyama Medical and Pharmaceutical University, Japan.

SO: Chem-Pharm-Bull-Tokyo. 1991 Mar; 39(3): 757-60

This title is owned by this library

ISSN: 0009-2363

LA: ENGLISH

AB: Eubacterium sp. strain BAR, isolated from human feces, transformed barbaloin to aloe-emodin anthrone in a basal medium lacking carbohydrate. Barbaloin remarkably stimulated the growth of strain BAR in the basal medium, the stimulative extent of the growth depending on the amount of barbaloin added. The addition of D-glucose, D-galactose, maltose, cellobiose, sucrose or D-amygdalin to the basal medium containing barbaloin caused a decrease of the growth stimulated by barbaloin to the growth level with each sugar, resulting in a complete inhibition of the barbaloin transformation. On the other hand, the addition of D-fructose, which itself stimulated the growth of strain BAR, further increased the growth in the presence of barbaloin and little inhibited barbaloin transformation. Nojirimycin bisulfite, a specific inhibitor of glucosidases, potently inhibited the growth with barbaloin, but did not affect the growth with glucose or cellobiose. Also, nojirimycin bisulfite completely inhibited the transformation of barbaloin to aloe-emodin anthrone. These results indicate that a unique enzyme capable of cleaving the C-glycosyl bond is induced in strain BAR by barbaloin and, consequently, strain BAR grows by utilizing as a nutrient the carbohydrate liberated from barbaloin. It is further suggested that the barbaloin-cleaving enzyme is inhibited by nojirimycin bisulfite and that the induction of the enzyme is repressed with D-glucose and D-galactose.

AN: 91300606

MEDLINE EXPRESS (R) 1991 - 1995 217 of 324

TI: Topical use of aloe vera derived allantoin gel in otolaryngology [letter]

AU: Thompson-JE

SO: Ear-Nose-Throat-J. 1991 Jan; 70(1): 56

ISSN: 0145-5613

LA: ENGLISH

AN: 91293013

MEDLINE EXPRESS (R) 1991 - 1995 218 of 324

TI: Isolation of a human intestinal bacterium capable of transforming barbaloin to aloe-emodin anthrone.

AU: Che-QM; Akao-T; Hattori-M; Kobashi-K; Namba-T

AD: Research Institute for Wakan-Yaku (Traditional Sino-Japanese Medicines), Toyama Medical and Pharmaceutical University, Japan.

SO: Planta-Med. 1991 Feb; 57(1): 15-9

This title is owned by this library

ISSN: 0032-0943

LA: ENGLISH

AB: A strictly anaerobic bacterium, Eubacterium sp. BAR, was isolated from human feces as one of the intestinal bacteria capable of metabolizing barbaloin. The bacterium grew in PYF broth containing barbaloin and converted barbaloin to aloe-emodin anthrone. On the other hand, the bacterium had little metabolic activity in GAM broth.

AN: 91288685

MEDLINE EXPRESS (R) 1991 - 1995 219 of 324

TI: The isolation of an active inhibitory system from an extract of aloe vera.

AU: Davis-RH; Parker-WL; Samson-RT; Murdoch-DP

AD: Pennsylvania College of Podiatric Medicine, Philadelphia 19107.

SO: J-Am-Podiatr-Med-Assoc. 1991 May; 81(5): 258-61

ISSN: 8750-7315

LA: ENGLISH

AB: An Aloe vera extract was prepared with 50% ethanol. The resultant supernatant and precipitate were tested for anti-inflammatory activity using the croton oil-induced ear-swelling assay. The supernatant fraction decreased inflammation, when applied topically, by 29.2%, and the precipitate decreased inflammation by 12.1%. The authors have shown that the anti-inflammatory activity (inhibitory system) resides in the supernatant of a 50% ethanol extract.

AN: 91286962

MEDLINE EXPRESS (R) 1991 - 1995 220 of 324

TI: Aloe vera dermal wound gel is associated with a delay in wound healing.

AU: Schmidt-JM; Greenspoon-JS

AD: Department of Nursing, Women's Hospital, Los Angeles County-University of Southern California Medical Center.

SO: Obstet-Gynecol. 1991 Jul; 78(1): 115-7

This title is owned by this library

ISSN: 0029-7844

LA: ENGLISH

AB: We evaluated the time interval required for wound healing using a standard wound management protocol with and without aloe vera gel. Twenty-one women were studied who had wound complications requiring healing by second intention after cesarean delivery or laparotomy for gynecologic surgery. Wounds treated with standard management healed in a mean (+/- SD) time interval of 53 +/- 24 days, whereas those treated with aloe vera gel required 83 +/- 28 days (P = .003). The use of aloe vera dermal wound gel was associated with a significant delay in wound healing compared with treatment with an otherwise identical regimen that did not include aloe vera.

AN: 91261319

MEDLINE EXPRESS (R) 1991 - 1995 221 of 324

TI: Topical use of aloe vera derived allantoin gel in otolaryngology [letter]

AU: Thompson-JE

SO: Ear-Nose-Throat-J. 1991 Feb; 70(2): 119

ISSN: 0145-5613

LA: ENGLISH

AN: 91256902

MEDLINE EXPRESS (R) 1991 - 1995 222 of 324

TI: Adverse reactions to vitamin E and aloe vera preparations after dermabrasion and chemical peel.

AU: Hunter-D; Frumkin-A

AD: College of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City.

SO: Cutis. 1991 Mar; 47(3): 193-6

This title is owned by this library

ISSN: 0011-4162

LA: ENGLISH

AB: Three women and one man aged forty-one to sixty-five years experienced a severe burning sensation following the application of aloe vera or vitamin E preparations to a skin area that had been subjected to a chemical peel or dermabrasion. Subsequently, a severe dermatitis occurred that required hospitalization of one patient and intravenous administration of steroids. The dermatitis abated very slowly in all patients: full recovery took three months or more. One patient resumed the use of vitamin E creams two years after the episode of dermatitis and experienced no adverse effect. Patients undergoing dermabrasion or chemical peel procedures should be cautioned specifically against the use of aloe vera or vitamin E topically in the first weeks after surgery.

AN: 91215945

MEDLINE EXPRESS (R) 1991 - 1995 223 of 324

TI: Aloe vera as a biologically active vehicle for hydrocortisone acetate.

AU: Davis-RH; Parker-WL; Murdoch-DP

AD: Pennsylvania College of Podiatric Medicine, Philadelphia 19107.

SO: J-Am-Podiatr-Med-Assoc. 1991 Jan; 81(1): 1-9

ISSN: 8750-7315

LA: ENGLISH

AB: Aloe vera, as a biological vehicle for hydrocortisone 21-acetate, was tested topically and systemically against acute inflammation. Systemically, the combination of A. vera and hydrocortisone produced a maximum 88.1% inhibition of edema. Polymorphonuclear leukocyte infiltration was reduced 91.1%. The topical inhibition of edema peaked at 97%. The possibility that A. vera has significant potential as a biologically active vehicle for steroids is discussed.

AN: 91132421

MEDLINE EXPRESS (R) 1991 - 1995 224 of 324

TI: Comparisons of diet and biochemical characteristics of stool and urine between Chinese populations with low and high colorectal cancer rates.

AU: Yeung-KS; McKeown-Eyssen-GE; Li-GF; Glazer-E; Hay-K; Child-P; Gurgin-V; Zhu-SL; Baptista-J; Aloe-M; et-al

AD: Ludwig Institute for Cancer Research, Toronto, ON, Canada.

SO: J-Natl-Cancer-Inst. 1991 Jan 2; 83(1): 46-50

This title is owned by this library

ISSN: 0027-8874

LA: ENGLISH

AB: In an investigation of the roles of diet and stool biochemistry in human colorectal carcinogenesis, 24-hour food, urine, and stool samples were collected from randomly selected participants from two populations with a fourfold difference in colorectal cancer risk: Chinese in Sha Giao, People's Republic of China (low risk), and Chinese-Americans of similar ages in San Francisco County, Calif, in the United States (high risk). The findings supported the hypotheses that colorectal cancer risk is increased by the consumption of high-fat, high-protein, and low-carbohydrate diets and is associated with high levels of cholesterol in stool as well as increased daily outputs of 3-methyl-histidine and malonaldehyde in urine. However, risk does not increase with low stool bulk and low total stool fibers.

AN: 91080187

MEDLINE EXPRESS (R) 1996 225 of 324

TI: Acceleration of the alcohol oxidation rate in rats with aloin, a quinone derivative of Aloe.

AU: Chung-JH; Cheong-JC; Lee-JY; Roh-HK; Cha-YN

AD: College of Pharmacy, Seoul National University, Korea.

SO: Biochem-Pharmacol. 1996 Nov 8; 52(9): 1461-8

This title is owned by this library

ISSN: 0006-2952

LA: ENGLISH

AB: Aloe contains abundant aloin, a C-glycoside derivative of anthraquinone. Based on recent reports indicating that the water extract of Aloe enhances the ethanol oxidation rate and also that quinones, in general, have a functional role in elevating the alcohol oxidation rate in vivo, we have attempted to identify the quinone derivative contained in Aloe that could increase the alcohol oxidation rate. Upon oral administration of aloin (300 mg/kg) given 12 hr prior to the administration of alcohol (3.0 g/kg), the blood alcohol area under the curve (AUC) was found to be decreased significantly (by 40%). This was supported by increases in the rates of blood alcohol elimination and the disappearance of alcohol from the body by 45 and 50%, respectively. Analysis of hepatic triglyceride (TG) levels revealed that both the ethanol and the aloin treatment alone significantly increased the TG levels in a comparable manner; however, the level obtained by the combined treatment of aloin and ethanol was not statistically different from that produced by either treatment alone. The levels of serum L-aspartate:2-oxoglutarate aminotransferase (AST) and L-alanine:2-oxoglutarate aminotransferase (ALT) activities were not increased by acute alcohol intoxication, aloin alone, or by the combined treatment of alcohol and aloin. Pretreatments with aloe-emodin, the anthraquinone aglycone of aloin, resulted in a significantly decreased blood alcohol AUC and an increase in the rate of ethanol disappearance. These results suggested that when the aloin localized primarily in the skin of Aloe is ingested, aloe-emodin (the quinone aglycone) may be released, and the released quinone may produce acceleration of the ethanol metabolism rate in vivo.

AN: 97091792

MEDLINE EXPRESS (R) 1996 226 of 324

TI: Levels of NGF, p75NGFR and ChAT immunoreactivity in brain of adult and aged microencephalic rats.

AU: Cimino-M; Cattabeni-F; Di-Luca-M; Peruzzi-G; Andena-M; Tirassa-P; Angelucci-F; Cozzari-C; Aloe-L

AD: Institute of Pharmacology and Pharmacognosy, University of Urbino, Chiara, Italy.

SO: Neurobiol-Aging. 1996 Jan-Feb; 17(1): 137-42

This title is owned by this library

ISSN: 0197-4580

LA: ENGLISH

AB: Methylazoxymethanol (MAM)-induced microencephalic aged animals with reduced cortical mass and unmodified basal nucleus were used to study the relationship between cells that produce and cells that utilize NGF. Total cortical ChAT activity of MAM 2, 19 and 27 month old animals was reduced compared to their age-matched controls. To verify whether the reduction of enzyme activity can be ascribed to changes in or ablation of projecting neurons, we carried out immunohistochemical analysis of ChAT and low affinity NGF receptor (p75NGFR) in the basal nucleus of control and MAM-treated animals. ChAT and p75NGFR immunostaining of basal forebrain cholinergic neurons showed morphological changes in MAM animals, as revealed by cellular atrophy, reduced dendritic arborization and decreased staining intensity. In the cerebral cortex of microencephalic animals, reduced levels of NGF compared to controls were observed at all examined ages. These results suggest that MAM treatment induces long-lasting ablation of cortical NGF-synthesizing cells leading to reduced trophic support to basal forebrain cholinergic neurons, which might be responsible for the cellular atrophy observed in the basal nucleus.

AN: 96246769

MEDLINE EXPRESS (R) 1996 227 of 324

TI: Excessive somnolence.

AU: Tavares-S; Aloe-F; Gentil-V; Scaff-M

AD: Interdepartmental Center for Sleep Studies, Hospital das Clinicas, University of Sao Paulo (CIES HC-FMUSP), Brazil.

SO: Rev-Paul-Med. 1996 Jul-Aug; 114(4): 1231-8

This title is owned by this library

ISSN: 0035-0362

LA: ENGLISH

AB: Excessive somnolence can be quite a incapacitating manifestation, and is frequently neglected by physicians and patients. This article reviews the determinant factors, the evaluation and quantification of diurnal somnolence, and the description and treatment of the main causes of excessive somnolence.

AN: 97340552

MEDLINE EXPRESS (R) 1996 228 of 324

TI: Nerve growth factor circulating levels are increased in Kawasaki disease: correlation with disease activity and reduced angiotensin converting enzyme levels.

AU: Falcini-F; Cerinic-MM; Ermini-M; Generini-S; Lombardi-A; Pignone-A; Leoncini-G; Tirassa-P; Aloe-L

AD: Department of Pediatrics, University of Florence, Italy.

SO: J-Rheumatol. 1996 Oct; 23(10): 1798-802

This title is owned by this library

ISSN: 0315-162X

LA: ENGLISH

AB: OBJECTIVE: To investigate the serum levels of nerve growth factor (NGF) and their possible relationship with disease activity in Kawasaki disease. METHODS: Twelve children with Kawasaki disease, 12 healthy children (afebrile controls), and 12 children with high fever related to upper respiratory tract viral infections (URTVI) were enrolled in the study. Serum NGF levels were measured by an ELISA method before and after therapy. In addition, angiotensin converting enzyme (ACE), and erythrocyte sedimentation rate (ESR), complete hemochrome, and C-reactive protein were tested as indicators of endothelial injury and disease activity, respectively. RESULTS: NGF levels were strikingly increased in the acute phase of Kawasaki disease (1219.54 +/- 1660.29 pg/ml) and decreased in the inactive phase of the disease (277.08 +/- 245.64 pg/ml), while remaining significantly higher than controls (6.5 +/- 2.03 pg/ml). In febrile patients with URTVI, NGF levels (30.18 +/- 44.70 pg/ml) were moderately but significantly increased compared to afebrile controls, but remarkably lower in respect to active and inactive Kawasaki disease. In active Kawasaki disease, ACE values were significantly lower than in healthy children (2.8 +/- 1.7 vs 10.1 +/- 4.2 pmol/ml/min; p < 0.001) and increased during the inactive phase, but remaining lower than in controls (4.8 +/- 2.7 pmol/ml/min); ESR, hemoglobin level, and platelet count showed significant correlation with NGF, while ACE levels showed a significant inverse correlation with ESR and NGF. CONCLUSION: In Kawasaki disease, NGF increased together with decreased ACE may be linked to a diffuse vascular inflammatory process. NGF and ACE levels remained abnormal even when there was no clinical sign of disease activity. This may indicate that the disease process is not in complete remission and suggests careful and prolonged cardiac followup until their normalization.

AN: 97050434

MEDLINE EXPRESS (R) 1996 229 of 324

TI: Evidence of a role for nerve growth factor in the effect of sialoadenectomy on body temperature of parasite-infected mice.

AU: Aloe-L; Moroni-R; Angelucci-F

AD: Institute of Neurobiology, Rome, Italy.

SO: Arch-Oral-Biol. 1996 Jan; 41(1): 21-6

This title is owned by this library

ISSN: 0003-9969

LA: ENGLISH

AB: Mice infected with Schistosoma mansoni were used to investigate the role of the submaxillary salivary gland and nerve growth factor (NGF) in temperature response. The results showed that the infection increased (36.5 +/- 0.3 vs 35.7 +/- 0.2), while sialoadenectomy decreased (34.4 +/- 0.2 vs 35.1 +/- 0.2) body temperature. These temperature changes were associated with high or low circulating NGF levels, respectively. It was also found that infection altered the distribution of oxytocin-positive neurones in the hypothalamus and that administration of 20 mu g of purified NGF in normal mice raised (36.1 +/- 0.2 vs 35.1 +/- 0.2) and of NGF antibodies decreased (34.0 +/- 0.2 vs 35.1 +/- 0.2) body temperature. Taken together, these observations suggest that salivary NGF influences the temperature set-point in adult rodents, but the mechanism regulating these events remains to be elucidated.

AN: 96430461

MEDLINE EXPRESS (R) 1996 230 of 324

TI: Isolation of a cDNA for a phosphoenolpyruvate carboxylase from a monocot CAM-plant, Aloe arborescens: structure and its gene expression.

AU: Honda-H; Okamoto-T; Shimada-H

AD: Life Science Institute, Mitsui Toatsu Chemicals, Inc., Mobara, Japan.

SO: Plant-Cell-Physiol. 1996 Sep; 37(6): 881-8

ISSN: 0032-0781

LA: ENGLISH

AB: A phosphoenolpyruvate carboxylase (PEPCase) cDNA was isolated from Aloe arborescens, a monocot CAM plant. Northern analysis of the PEPCase transcript indicated that it is specifically expressed in green leaves, strongly suggesting its involvement in CAM photosynthesis. No diurnal change in expression level was evident. Western blot analysis also showed no alteration of the amount of the PEPCase protein. These results suggest that circadian rhythm in PEPCase activity may be regulated post-translationally. The representative cDNA clone contained an ORF encoding 964 amino acid residues. Deduced amino acid sequence of the aloe PEPCase is highly conserved as compared with other PEPCases. The phosphorylation site which may be modified by PEPC-kinase was conserved. An evolutional map with known PEPCases suggested that CAM-type PEPCases were located between C4 and housekeeping PEPCases.

AN: 97043427

MEDLINE EXPRESS (R) 1996 231 of 324

TI: Antiinflammatory activity of extracts from Aloe vera gel.

AU: Vazquez-B; Avila-G; Segura-D; Escalante-B

AD: Laboratory of Pharmacology, Escuela Nacional de Estudios Profesionales Iztacala (E.N.E.P-I), Universidad Nacional Autonoma de Mexico, Tlalnepantla, Mexico.

SO: J-Ethnopharmacol. 1996 Dec; 55(1): 69-75

This title is owned by this library

ISSN: 0378-8741

LA: ENGLISH

AB: We studied the effects of aqueous, chloroform, and ethanol extracts of Aloe vera gel on carrageenan-induced edema in the rat paw, and neutrophil migration into the peritoneal cavity stimulated by carrageenan. We also studied the capacity of the aqueous extract to inhibit cyclooxygenase activity. The aqueous and chloroform extracts decreased the edema induced in the hind-paw and the number of neutrophils migrating into the peritoneal cavity, whereas the ethanol extract only decreased the number of neutrophils. The antiinflammatory agents indomethacin and dexamethasone also decreased carrageenan-induced edema and neutrophil migration. The aqueous extract inhibited prostaglandin E2 production from [14C]arachidonic acid. The chemical tests performed in the aqueous extract for anthraglycosides, reductor sugars and cardiotonic glycosides were positive. In the ethanol extract, the chemical tests performed for saponins, carbohydrates naftoquinones, sterols, triterpenoids and anthraquinones were also positive. In the chloroform extract, the chemical tests performed for sterols type delta 5, and anthraquinones were positive. These results demonstrated that the extracts of Aloe vera gel have antiinflammatory activity and suggested its inhibitory action on the arachidonic acid pathway via cyclooxygenase.

AN: 97184847

MEDLINE EXPRESS (R) 1996 232 of 324

TI: Isozymes of superoxide dismutase from Aloe vera.

AU: Sabeh-F; Wright-T; Norton-SJ

AD: Department of Biological Sciences, University of North Texas, Denton 76203, USA.

SO: Enzyme-Protein. 1996; 49(4): 212-21

This title is owned by this library

ISSN: 1019-6773

LA: ENGLISH

AB: Extracts from the parenchymatous leaf gel and the rind of the Aloe vera plant (Aloe barbadensis Miller) were shown to contain seven electrophoretically-identifiable superoxide dismutases (SODs). The chromatographic elution profiles and the migration of these bands on native polyacrylamide gel electrophoresis (PAGE), for both the gel and rind, are quite similar. Two of these seven activities are insensitive to cyanide treatment, suggesting that they are mangano-SODs. The other five activities are sensitive to cyanide treatment, but insensitive to azide treatment and are presumed to be cupro-zinc SODs. All of the seven proteins appear to be homodimers with apparent native molecular masses centered at approximately 32 and 42 kD as indicated by SDS-PAGE and gel-filtration (FPLC) chromatography. The specific activities of SODs in the A. vera rind and gel are comparable to those of spinach leaves and of rabbit liver.

AN: 97182749

MEDLINE EXPRESS (R) 1996 233 of 324

TI: Activation of a mouse macrophage cell line by acemannan: the major carbohydrate fraction from Aloe vera gel.

AU: Zhang-L; Tizard-IR

AD: Department of Veterinary Pathobiology, Texas A & M University College Station 77843, USA.

SO: Immunopharmacology. 1996 Nov; 35(2): 119-28

This title is owned by this library

ISSN: 0162-3109

LA: ENGLISH

AB: Acemannan is the name given to the major carbohydrate fraction obtained from the gel of the Aloe vera leaf. It has been claimed to have several important therapeutic properties including acceleration of wound healing, immune stimulation, anti-cancer and anti-viral effects. However, the biological mechanisms of these activities are unclear. Because of this wide diversity of effects, it is believed that they may be exerted through pluripotent effector cells such as macrophages. The effects of acemannan on the mouse macrophage cell line, RAW 264.7 cells were therefore investigated. It was found that acemannan could stimulate macrophage cytokine production, nitric oxide release, surface molecule expression, and cell morphologic changes. The production of the cytokines IL-6 and TNF-alpha were dependent on the dose of acemannan provided. Nitric oxide production, cell morphologic changes and surface antigen expression were increased in response to stimulation by a mixture of acemannan and IFN-gamma. These results suggest that acemannan may function, at least in part, through macrophage activation.

AN: 97115605

MEDLINE EXPRESS (R) 1996 234 of 324

TI: Genotoxicity of the laxative drug components emodin, aloe-emodin and danthron in mammalian cells: topoisomerase II mediated?

AU: Muller-SO; Eckert-I; Lutz-WK; Stopper-H

AD: Department of Toxicology, University of Wurzburg, Germany.

SO: Mutat-Res. 1996 Dec 20; 371(3-4): 165-73

This title is owned by this library

ISSN: 0027-5107

LA: ENGLISH

AB: 1,8-Dihydroxyanthraquinones are under debate as plant-derived carcinogens that are found in laxatives, food colors, and possibly vegetables. Published genotoxicity data are controversial, and so three of them (emodin, danthron and aloe-emodin) were tested in a number of in vitro assay systems. All three compounds induced tk-mutations in mouse lymphoma L5178Y cells. Induction of micronuclei also occurred in the same cell line, and was dose-dependent, with the potency ranking being danthron > aloe-emodin > emodin. In a DNA decatenation assay with a network of mitochondrial DNA of C. fasciulata, all three test compounds inhibited the topoisomerase II-mediated decatenation. Danthron and aloe-emodin, but not emodin, increased the fraction of DNA moving into comet tails when tested at concentrations around 50 microM in single-cell gel-electrophoresis assays (SCGE; comet assay). Comet assays were also used in modified form to determine whether pretreatment of the cells with the test compounds would reduce the effects of etoposide, a potent topoisomerase II inhibitor. All three test chemicals were effective in this pretreatment protocol, with danthron again being the most potent. Given clearcut evidence of their genotoxic activity, further research on the human cancer risk of these compounds may be warranted.

AN: 97161463

MEDLINE EXPRESS (R) 1996 235 of 324

TI: Hematopoietic augmentation by a beta-(1,4)-linked mannan.

AU: Egger-SF; Brown-GS; Kelsey-LS; Yates-KM; Rosenberg-LJ; Talmadge-JE

AD: Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-5660, USA.

SO: Cancer-Immunol-Immunother. 1996 Dec; 43(4): 195-205

This title is owned by this library

ISSN: 0340-7004

LA: ENGLISH

AB: CARN 750 (injectable acemannan) is a polydispersed beta-(1,4)-linked acetylated mannan isolated from the Aloe barbadensis plant. It has multiple therapeutic properties including activity in wound repair and as a biological agent for the treatment of neoplasia in animals as well as the ability to activate macrophages. We report herein that CARN 750 directly or indirectly has significant hematoaugmenting properties. We observed that the subcutaneous administration of CARN 750 significantly increases splenic and peripheral blood cellularity, as well as hematopoietic progenitors in the spleen and bone marrow as determined by the interleukin-3-responsive colony-forming unit culture assay and the high-proliferative-potential colony-forming-cell (HPP-CFC) assay (a measure of primitive hematopoietic precursors) in myelosuppressed (7 Gy) C57BL/6 mice. The greatest hematopoietic effect was observed following sublethal irradiation in mice receiving 1 mg CARN 750/ animal, with less activity observed at higher or lower doses. Further, CARN 750, following daily injection, has activity equal to or greater than the injection of an optimal dose of granulocyte-colony-stimulating factor (G-CSF) in myelosuppressed mice. In this comparison, significantly greater activity was observed in the splenic and peripheral blood cellularity, and in the frequency and absolute number of splenic HPP-CFC as compared to the mice receiving G-CSF at 3 micrograms/animal. CARN 750, when administered to myelosuppressed animals, decreased the frequency of lymphocytes with a concomitant significant increase in the frequency of polymorphonuclear leukocytes (PMN). However, owing to the increased cellularity, a significant increase in the absolute number of PMN, lymphocytes, monocytes and platelets was observed, suggesting activity on multiple cell lineages. The latter is the primary difference in activity as compared to G-CSF which has activity predominantly on PMN.

AN: 97157083

MEDLINE EXPRESS (R) 1996 236 of 324

TI: Centrally injected nerve growth factor modulates peripheral immune responses in the rat.

AU: Sacerdote-P; Manfredi-B; Aloe-L; Micera-A; Panerai-AE

AD: Department of Pharmacology, University of Milan, Italy.

SO: Neuroendocrinology. 1996 Oct; 64(4): 274-9

This title is owned by this library

ISSN: 0028-3835

LA: ENGLISH

AB: The effect of the intracerebroventricular (icv) and intravenous (iv) injection of nerve growth factor (NGF) on peripheral immunity was studied in the rat. Icv administration of NGF (5, 25, 50, 250 and 500 ng/rat) significantly enhanced phytohemagglutinin (PHA)-induced splenocyte proliferation 30 min after treatment. Icv pretreatment with an anti-NGF antibody completely prevented the effect, while iv injection of anti-NGF antibody did not block the effect of icv NGF. On the contrary, NGF at doses of 0.5, 2.5, 5, 25 and 50 ng/rat decreased splenocyte natural killer (NK) activity. When injected iv, NGF enhanced splenocyte proliferation only at doses of 50 and 500 ng/rat, while it did not affect NK activity. These effects on immunity do not appear mediated by activation of the hypothalamus-pituitary-adrenal axis, since NGF did not modify plasma corticosterone concentrations at the doses used. These results indicate that NGF participates in the complex network of neuroimmune interactions.

AN: 97051117

MEDLINE EXPRESS (R) 1996 237 of 324

TI: Chronic parasite infection in mice induces brain granulomas and differentially alters brain nerve growth factor levels and thermal responses in paws.

AU: Aloe-L; Moroni-R; Fiore-M; Angelucci-F

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: Acta-Neuropathol-Berl. 1996 Sep; 92(3): 300-5

This title is owned by this library

ISSN: 0001-6322

LA: ENGLISH

AB: Schistosoma mansoni infection, both in humans and in animal models, is known to induce granulomas in the liver and intestine. It has also been reported that in humans the eggs of this parasite can reach the brain, causing psychiatric and neuropathological disorders. Whether this also occurs in rodents is unknown. To answer this question, mice were infected with this parasite and the central nervous system (CNS) examined at various time intervals. The results show that schistosomiasis induced granulomas in several regions of the CNS and increased nerve growth factor (NGF) levels in the cortex, hypothalamus and brain stem, but not in the hippocampus. The infection also caused paw hyperalgesia, as determined by the hot-plate test, and a local increase in NGF, but not in substance P. These findings indicate that the murine model of infection can be used for studying mechanisms leading to human neuroschistosomiasis and suggest that the neuropathological disorders and the sensory deficits observed in human schistosomiasis are associated with impaired levels of NGF in the peripheral and central nervous system.

AN: 97024605

MEDLINE EXPRESS (R) 1996 238 of 324

TI: Modification of lymphoid and brain nerve growth factor levels in systemic lupus erythematosus mice.

AU: Bracci-Laudiero-L; Lundeberg-T; Stenfors-C; Theodorsson-E; Tirassa-P; Aloe-L

AD: Institute of Neurobiology, Rome, Italy.

SO: Neurosci-Lett. 1996 Feb 2; 204(1-2): 13-6

This title is owned by this library

ISSN: 0304-3940

LA: ENGLISH

AB: In the present work we investigated the production of nerve growth factor (NGF) in the brain and peripheral tissues of female NZB/W F1 mice, a well characterized model of murine lupus. Our results indicate that while no significant difference in the NGF content was observed in the sera and tissues of NZB/W mice and its parental strains during the first months of life, the levels of circulating NGF and the NGF content in the kidneys significantly increase in the autoimmune mice during the development of the disease. The NGF-producing brain regions showed a decrease in NGF concentration in 8 month-old NZB/W mice. Moreover, we found a modification of the NGF concentration in the spleens of autoimmune mice at 5 and 8 months. Our data support the hypothesis of a correlation between NGF and the inflammatory state of systemic lupus erythematosus (SLE) and indicate that NGF could have a role in the pathogenesis of this disease.

AN: 97083392

MEDLINE EXPRESS (R) 1996 239 of 324

TI: Nerve growth factor delays retinal degeneration in C3H mice.

AU: Lambiase-A; Aloe-L

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: Graefes-Arch-Clin-Exp-Ophthalmol. 1996 Aug; 234 Suppl 1: S96-100

This title is owned by this library

ISSN: 0721-832X

LA: ENGLISH

AB: BACKGROUND: The aim of the present study was to investigate the biological role of nerve growth factor (NGF) on retinal degeneration in the C3H mouse strain. This strain is characterized by a single gene mutation (rd) which leads to photoreceptor degeneration resembling human retinitis pigmentosa. METHODS: Neural retinas from 1- to 25 day-old C3H mice were dissected from outer ocular tissues, dissociated in cell suspension, stained with a vital dye and counted in a hemocytometer. For in vivo study, NGF was injected into the intraocular or retro-ocular area, and at the end of the treatment the mice were killed. The eyes were enucleated, fixed and cut by cryostat into 14-microns serial sections. The serial sections were stained with hematoxylin-eosin and the outer nuclear layer (ONL) was measured using a computerized image analysis system. RESULTS: An intraocular injection of NGF, or repeated retro-ocular injections, induced a significant increase in ONL thickness compared to controls. CONCLUSION: Our data show that NGF inhibits retinal degeneration in C3H mice. The mechanism(s) underlying the protective action of NGF against retinal cell death remains to be established.

AN: 97024933

MEDLINE EXPRESS (R) 1996 240 of 324

TI: Histopathological evaluation of retinal damage during intraocular hypertension in rabbit: involvement of ganglion cells and nerve fiber layer.

AU: Manni-G; Lambiase-A; Centofanti-M; Mattei-E; De-Gregorio-A; Aloe-L; de-Feo-G

AD: Department of Ophthalmology, University of Rome, Tor Vergata, Italy.

SO: Graefes-Arch-Clin-Exp-Ophthalmol. 1996 Aug; 234 Suppl 1: S209-13

This title is owned by this library

ISSN: 0721-832X

LA: ENGLISH

AB: BACKGROUND: Current clinical tests do not detect glaucomatous signs until the onset of substantial retinal damage. Therefore animal models are required to investigate the very early histopathological alterations in glaucoma disease. We used an experimental model of intraocular hypertension to compare early changes in retinal ganglion cell (RGC) density with the thickness of the nerve fiber layer (NFL). METHODS: Methylcellulose 2% was injected into the anterior chamber of 18 eyes of 18 New Zealand albino rabbits. Intraocular pressure was measured 6 h after the injection and thenceforth once a day using a Shiotz tonometer. Histopathological analysis was performed on days 4, 10, and 15 following the induction of hypertension (six eyes for each group). Sections from the upper temporal retina were stained with hematoxylin-eosin and immunohistochemically using a polyclonal antibody PGP 9.5 to identify RGC. An image analysis system was used to evaluate the RGC and the thickness of the NFL. RESULTS: We observed a significant increase in intraocular pressure until the end of the experiment. Histological analysis showed, after 10 days of ocular hypertension, a significant decrease in RGC density (P < 0.05) and a significant increase (P < 0.05) in glial cell density. We found a significant correlation between RGC loss and cell area at 4 days (P < 0.01; Cc = 0.86) and at 10 days (P < 0.002; Cc = 0.91) of intraocular hypertension. We did not observe a significant decrease in the NFL thickness until 10 days of intraocular hypertension. CONCLUSIONS: Our study confirms the size-dependent RGC loss during intraocular hypertension and shows no early decrease in NFL thickness.

AN: 97024952

MEDLINE EXPRESS (R) 1996 241 of 324

TI: Increased circulating nerve growth factor is directly correlated with disease activity in juvenile chronic arthritis.

AU: Falcini-F; Matucci-Cerinic-M; Lombardi-A; Generini-S; Pignone-A; Tirassa-P; Ermini-M; Lepore-L; Partsch-G; Aloe-L

AD: Department of Paediatrics, University of Florence, Italy.

SO: Ann-Rheum-Dis. 1996 Oct; 55(10): 745-8

This title is owned by this library

ISSN: 0003-4967

LA: ENGLISH

AB: OBJECTIVE: To determine the circulating serum concentrations of nerve growth factor (NGF) and compare them with indices of disease activity in juvenile chronic arthritis. METHODS: NGF concentrations were evaluated with a two site immunoenzymatic assay (ELISA), in 17 children with systemic, 39 with polyarticular, and 24 with pauciarticular onset juvenile chronic arthritis. Each subset was divided according to different variables, appropriate to each subset, reflecting active and inactive disease. RESULTS: NGF concentrations were significantly higher in children with systemic [254 (SD 256.1) pg ml-1; P < 0.001], polyarticular [165.2 (300.8) pg ml-1; P < 0.05], and pauciarticular [106.8 (111.8) pg ml-1; P < 0.005] onset juvenile chronic arthritis than in controls. In all subsets, NGF concentrations were higher in the active than in the inactive phase of the disease. A significant direct correlation between NGF concentrations and erythrocyte sedimentation rate was found both in the systemic and in the polyarticular onset juvenile chronic arthritis. CONCLUSIONS: The increase in NGF concentrations in all juvenile chronic arthritis subsets and the correlation with disease activity suggest that NGF may take an active part in joint inflammation.

AN: 97105181

MEDLINE EXPRESS (R) 1996 242 of 324

TI: Haloperidol treatment decreases nerve growth factor levels in the hypothalamus of adult mice.

AU: Alleva-E; Della-Seta-D; Cirulli-F; Aloe-L

AD: Section of Behavioral Pathophysiology, Istituto Superiore di Sanita, Rome, Italy.

SO: Prog-Neuropsychopharmacol-Biol-Psychiatry. 1996 Apr; 20(3): 483-9

ISSN: 0278-5846

LA: ENGLISH

AB: 1. In a first study different doses of haloperidol (0.6, 1.2, 2.5, 5, 10, or 20 mg/kg; intraperitoneally) were administered to adult male mice (CD-1 strain) and tested for their ability to induce catalepsy. 2. The minimal haloperidol dose inducing complete catalepsy was found to be the 10 mg/kg dose and selected for the second experiment. 3. Using an immunoenzymatic assay (ELISA) hypothalamic nerve growth factor (NGF) level was measured 20 or 180 min following haloperidol injection (10 mg/kg). 4. Haloperidol treatment decreased NGF levels in mouse hypothalamus and this effect did not differ at the two time points tested. 5. The role of hypothalamic NGF in stress-related events is discussed.

AN: 96367489

MEDLINE EXPRESS (R) 1996 243 of 324

TI: mRNA for NGF and p75 in the central nervous system of rats affected by experimental allergic encephalomyelitis.

AU: De-Simone-R; Micera-A; Tirassa-P; Aloe-L

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: Neuropathol-Appl-Neurobiol. 1996 Feb; 22(1): 54-9

This title is owned by this library

ISSN: 0305-1846

LA: ENGLISH

AB: In this study we measured the concentration of nerve growth factor (NGF) and the expression of NGF and the low affinity NGF-receptor (NGF-r) mRNA in the central nervous system (CNS) of rats affected by experimental allergic encephalomyelitis (EAE) during the acute phase of the disease. Significant levels of NGF protein were found in thalamus and cortex on day 13 post-immunization. Molecular analysis of the NGF gene expression and of its NGF-r revealed that they were enhanced in several regions of the CNS of EAE rats when compared with untreated animals. These results suggest a functional link between local NGF synthesis and this autoimmune inflammatory disease.

AN: 97020404

MEDLINE EXPRESS (R) 1996 244 of 324

TI: Erythropoietin in myelodysplastic syndromes: durable response in a young patient [letter; comment] [see comments]

AU: Aloe-Spiriti-MA; Latagliata-R; Petti-MC

SO: Haematologica. 1996 Jul-Aug; 81(4): 381-2

This title is owned by this library

ISSN: 0390-6078

LA: ENGLISH

AN: 97024070

MEDLINE EXPRESS (R) 1996 245 of 324

TI: Neurobehavioral alterations in developing transgenic mice expressing TNF-alpha in the brain.

AU: Fiore-M; Probert-L; Kollias-G; Akassoglou-K; Alleva-E; Aloe-L

AD: Institute of Neurobiology, CNR, viale Marx 15, Rome, 00156, Italy.

SO: Brain-Behav-Immun. 1996 Jun; 10(2): 126-38

This title is owned by this library

ISSN: 0889-1591

LA: ENGLISH

AB: During development, neuronal circuitry and memory formation are associated with the synthesis and release of several biological mediators, including cytokines. Among the numerous cytokines, the role of tumor necrosis factor-alpha (TNF-alpha) in neurobehavioral development is largely unknown. Thus, the recently generated transgenic mice expressing murine TNF-alpha in the brain represent a valid animal model for investigating the role of TNF-alpha in neurobehavioral processes. Using these mice, we showed that an overexpression of murine TNF-alpha increases grooming in the novel object investigation test, decreases rearing as a reaction to novel olfactory cues, and produces a retardation of passive avoidance acquisition while enhancing the thermal response in the hot-plate test, a task regulated by both peripheral and central mechanisms. The possibility that these effects are associated with endogenous changes in concentration of the NGF, known to be modulated by TNF-alpha, is discussed.

AN: 96410706

MEDLINE EXPRESS (R) 1996 246 of 324

TI: Changes in human plasma nerve growth factor level after chronic alcohol consumption and withdrawal.

AU: Aloe-L; Tuveri-MA; Guerra-G; Pinna-L; Tirassa-P; Micera-A; Alleva-E

AD: Institute of Neurobiology, Rome, Italy.

SO: Alcohol-Clin-Exp-Res. 1996 May; 20(3): 462-5

ISSN: 0145-6008

LA: ENGLISH

AB: Numerous studies reported in recent years have shown that withdrawal from chronic consumption of drugs induces high levels of anxiety, both in humans and in animal models. In the present study, we demonstrated that withdrawal from chronic consumption of either ethanol or heroin causes a significant increase in plasma nerve growth factor, suggesting that the resulting anxiety condition triggers the release of this molecule. Although the functional significance of this phenomenon needs to be better defined, it is hypothesized that the increased levels of circulating nerve growth factor might be involved in homeostatic adaptive and/or reparative mechanisms.

AN: 96330462

MEDLINE EXPRESS (R) 1996 247 of 324

TI: Circulating nerve growth factor levels are increased in humans with allergic diseases and asthma.

AU: Bonini-S; Lambiase-A; Bonini-S; Angelucci-F; Magrini-L; Manni-L; Aloe-L

AD: Department of Allergology and Clinical Immunology, Second University of Naples, Italy.

SO: Proc-Natl-Acad-Sci-U-S-A. 1996 Oct 1; 93(20): 10955-60

This title is owned by this library

ISSN: 0027-8424

LA: ENGLISH

AB: Nerve growth factor (NGF) serum levels were measured in 49 patients with asthma and/or rhinoconjunctivitis and/or urticaria-angioedema. Clinical and biochemical parameters, such as bronchial reactivity, total and specific serum IgE levels, and circulating eosinophil cationic protein levels, were evaluated in relation to NGF values in asthma patients. NGF was significantly increased in the 42 allergic (skin-test- or radioallergosorbent-test-positive) subjects (49.7 +/- 28.8 pg/ml) versus the 18 matched controls (3.8 +/- 1.7 pg/ml; P < 0.001). NGF levels in allergic patients with asthma, rhinoconjunctivitis, and urticaria-angioedema were 132.1 +/- 90.8, 17.6 +/- 6.1, and 7.6 +/- 1.8 pg/ml (P < 0.001, P < 0.002, and P < 0.05 versus controls), respectively. Patients with more than one allergic disease had higher NGF serum values than those with a single disease. When asthma patients were considered as a group, NGF serum values (87.6 +/- 59.8 pg/ml) were still significantly higher than those of control groups (P < 0.001), but allergic asthma patients had elevated NGF serum levels compared with nonallergic asthma patients (132.1 +/- 90.8 versus 4.9 +/- 2.9 pg/ml; P < 0.001). NGF serum levels correlate to total IgE serum values (rho = 0.43; P < 0.02). The highest NGF values were found in patients with severe allergic asthma, a high degree of bronchial hyperreactivity, and high total IgE and eosinophil cationic protein serum levels. This study represents the first observation (that we know of) that NGF is increased in human allergic inflammatory diseases and asthma.

AN: 97008114

MEDLINE EXPRESS (R) 1996 248 of 324

TI: Use of plant-derived therapies in a rural, biracial population in Mississippi.

AU: Frate-DA; Croom-EM Jr; Frate-JB; Juergens-JP; Meydrech-EF

AD: Research Institute of Pharmaceutical Sciences, University of Mississippi, University, USA.

SO: J-Miss-State-Med-Assoc. 1996 Jan; 37(1): 427-9

ISSN: 0026-6396

LA: ENGLISH

AB: Exploring the use of plant-derived medicines has recently received much attention in both scientific and popular journals. A study conducted in central Mississippi on medicinal plants investigated the frequency of use, types of plants used, and symptoms treated. A randomly selected probability sample of 223 households found that over 70 percent of the adults used at least one plant-derived medicine during the past year. The most frequently used plants included lemon, aloe, castor, turpentine, tobacco, and garlic. Frequency of use varied by race with a higher proportion of African-Americans than Caucasians reporting use of a plant-derived medicine. Due to the possible presence of toxic compounds or potential drug interactions, it is recommended that health care professionals investigate the use of plant-derived therapies as part of the patient's medical history.

AN: 96418934

MEDLINE EXPRESS (R) 1996 249 of 324

TI: Phase III double-blind evaluation of an aloe vera gel as a prophylactic agent for radiation-induced skin toxicity.

AU: Williams-MS; Burk-M; Loprinzi-CL; Hill-M; Schomberg-PJ; Nearhood-K; O'Fallon-JR; Laurie-JA; Shanahan-TG; Moore-RL; Urias-RE; Kuske-RR; Engel-RE; Eggleston-WD

AD: Toledo Community Clinical Oncology Program, OH, USA.

SO: Int-J-Radiat-Oncol-Biol-Phys. 1996 Sep 1; 36(2): 345-9

This title is owned by this library

ISSN: 0360-3016

LA: ENGLISH

AB: PURPOSE: Considerable pilot data and clinical experience suggested that an aloe vera gel might help to prevent radiation therapy-induced dermatitis. METHODS AND MATERIALS: Two Phase III randomized trials were conducted. The first one was double blinded, utilized a placebo gel, and involved 194 women receiving breast or chest wall irradiation. The second trial randomized 108 such patients to aloe vera gel vs. no treatment. Skin dermatitis was scored weekly during both trials both by patients and by health care providers. RESULTS: Skin dermatitis scores were virtually identical on both treatment arms during both of the trials. The only toxicity from the gel was rare contact dermatitis. CONCLUSIONS: This dose and schedule of an aloe vera gel does not protect against radiation therapy-induced dermatitis.

AN: 97047538

MEDLINE EXPRESS (R) 1996 250 of 324

TI: Studies on optimal dose and administration schedule of a hematopoietic stimulatory beta-(1,4)-linked mannan.

AU: Egger-SF; Brown-GS; Kelsey-LS; Yates-KM; Rosenberg-LJ; Talmadge-JE

AD: Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-5660, USA.

SO: Int-J-Immunopharmacol. 1996 Feb; 18(2): 113-26

ISSN: 0192-0561

LA: ENGLISH

AB: Several complex carbohydrates have been found to significantly stimulate hematopoiesis. CARN 750, a polydispersed beta-(1,4)-linked acetylated mannan isolated from the Aloe vera plant, has been shown to have activity in wound repair, to function as a antineoplastic, and to activate macrophages. We report, herein, the hematoaugmenting properties of CARN 750 and its optimal dose and timing of administration in an animal model of irradiation-induced myelosuppression. We observed that subcutaneous injections of 1 mg/animal of CARN 750 had equal or greater stimulatory activity for white blood cell (WBC) counts and spleen cellularity as well as on the absolute numbers of neutrophils, lymphocytes, monocytes and platelets than did higher or lower doses of CARN 750 or an optimal dose of granulocyte-colony stimulating factor (G-CSF). Hematopoietic progenitors, measured as interleukin-3-supported colony forming units-culture (CFU-C) and high proliferative potential colony-forming cells (HPP-CFC) assays, were similarly increased by CARN 750 in the spleen but not in the bone marrow. The frequency of splenic HPP-CFCs and absolute number of splenic HPP-CFCs and CFU-Cs were optimally increased by 1 mg/animal of CARN 750. In contrast, bone marrow cellularity, frequency and absolute number of HPP-CFCs and CFU-Cs had as a dosage optimum 2 mg/animal of CARN 750. These parameters were similarly increased by G-CSF. In studies to determine the optimal protocol for the administration of CARN 750 we found that the hematopoietic activity of CARN 750 increased with the frequency of administration. The greatest activity in myelosuppressed mice was observed for all hematopoietic parameters except the platelet number in mice receiving daily administration of 1 mg/animal of CARN 750 with activity equal to or greater than G-CSF.

AN: 96392573

MEDLINE EXPRESS (R) 1996 251 of 324

TI: A purgative action of barbaloin is induced by Eubacterium sp. strain BAR, a human intestinal anaerobe, capable of transforming barbaloin to aloe-emodin anthrone.

AU: Akao-T; Che-QM; Kobashi-K; Hattori-M; Namba-T

AD: Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.

SO: Biol-Pharm-Bull. 1996 Jan; 19(1): 136-8

ISSN: 0918-6158

LA: ENGLISH

AB: Orally administered barbaloin (100 mg/kg) did not induce any diarrhea in male Wistar rats, in spite of severe diarrhea with sennoside B (40 mg/kg). Also, in gnotobiote rats mono-associated with Peptostreptococcus intermedius, a human intestinal anaerobe capable of reducing sennidins to rhein anthrone, barbaloin did not induce diarrhea; the faecal water content (71.9%) 8 h after the administration of barbaloin was not increased, compared with that (73.9%) just before the treatment. However, severe diarrhea was induced with barbaloin in gnotobiote rats mono-associated with Eubacterium sp. strain BAR, another human intestinal anaerobe capable of transforming barbaloin to aloe-emodin anthrone; the faecal water content was significantly increased to 85.5% 8 h after the administration, from 73.2% before the treatment. At this time, barbaloin was transformed to aloe-emodin anthrone in the feces from the gnotobiote rats mono-associated with the strain BAR, but not in feces from the conventional rats or the gnotobiote rats mono-associated with P. intermedius. These facts indicate that barbaloin is inactive as a laxative itself but is activated to aloe-emodin anthrone, a genuine purgative component, by Eubacterium sp. strain BAR.

AN: 96418141

MEDLINE EXPRESS (R) 1996 252 of 324

TI: Nerve growth factor stimulates production of neuropeptide Y in human lymphocytes.

AU: Bracci-Laudiero-L; Aloe-L; Stenfors-C; Tirassa-P; Theodorsson-E; Lundberg-T

AD: Institute of Neurobiology CNR, Rome, Italy.

SO: Neuroreport. 1996 Jan 31; 7(2): 485-8

ISSN: 0959-4965

LA: ENGLISH

AB: The production of neuropeptide Y (NPY) in lymphocytes obtained from human tonsils was investigated using radioimmunoassay. While unstimulated lymphocytes did not produce detectable amounts of NPY, NPY synthesis was induced after cell activation. Our results show that the addition of nerve growth factor (NGF) to unstimulated lymphocytes has an effect similar to that of mitogens, both leading to production of NPY. The study of purified B and T cells confirmed that only activated cells are able to synthesize NPY. The stimulatory effect of NGF on NPY production is not a common characteristic of all lymphocytes: only unstimulated T cells respond to NGF by synthesizing NPY. No such effects is seen in purified B cells.

AN: 96290650

MEDLINE EXPRESS (R) 1996 253 of 324

TI: Zinc acetate and lyophilized aloe barbadensis as vaginal contraceptive.

AU: Fahim-MS; Wang-M

AD: Center of Reproductive Science and Technology, School of Medicine, University of Missouri, Columbia 65212, USA.

SO: Contraception. 1996 Apr; 53(4): 231-6

ISSN: 0010-7824

LA: ENGLISH

AB: Twenty samples of fresh ejaculate, donated by healthy volunteers ranging in age from 20-30 years, were obtained from the Center for Fertility & Cryobiology, University of Missouri, Columbia, Missouri. Average semen volume was 2.49 ml; average sperm motility was 71.32%; and average sperm density was 113.71 x 10(6) /ml. Testing for spermicidal effectiveness of a 1% concentration of zinc acetate, zinc sulfate, zinc chloride, and zinc gluconate proved that only zinc acetate was spermicidal. It appears this is due to the acetate in zinc acetate which may decrease oxygen utilization by sperm. Zinc acetate in vitro was antiviral while lyophilized aloe barbadensis was not. Lyophilized aloe barbadensis at concentrations of 7.5% and 10% proved to be spermicidal due to the multiple micro elements (boron, barium, calcium, chromium, copper, iron, potassium, magnesium, manganese, phosphorus, and zinc) which were toxic to the tail causing instant immobilization. The two compounds did not irritate or cause ulceration of rabbit vaginal epithelium. These results suggest the possibility of using zinc acetate and lyophilized aloe barbadensis as a new, effective and safe vaginal contraceptive.

AN: 96276558

MEDLINE EXPRESS (R) 1996 254 of 324

TI: Decreased levels of 2-amino-3-methylimidazo[4,5-f]quinoline-DNA adducts in rats treated with beta-carotene, alpha-tocopherol and freeze-dried aloe.

AU: Uehara-N; Iwahori-Y; Asamoto-M; Baba-Toriyama-H; Iigo-M; Ochiai-M; Nagao-M; Nakayama-M; Degawa-M; Matsumoto-K; Hirono-I; Beppu-H; Fujita-K; Tsuda-H

AD: Chemotherapy Division, National Cancer Center Research Institute, Tokyo, Japan.

SO: Jpn-J-Cancer-Res. 1996 Apr; 87(4): 342-8

This title is owned by this library

ISSN: 0910-5050

LA: ENGLISH

AB: To assess mechanisms of chemoprevention of hepatocarcinogenesis by trans-beta-carotene (beta-C), DL-alpha-tocopherol (alpha-T), and freeze-dried whole leaves of Kidachi aloe (Aloe), formation of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-DNA adducts was measured by 32P-post-labeling analysis, and CYP1A1 and CYP1A2 protein levels were analyzed by ELISA. Group 1 rats were fed diet containing 0.02% beta-C, 1.5% alpha-T or 30% Aloe over an 8-day period, while group 2 was given basal diet alone. On day 7, all animals were subjected to two-thirds partial hepatectomy (PH). Twelve hours after PH, they received a single dose of the carcinogenic food pyrolysate IQ (100 mg/kg) intragastrically, to initiate hepatocarcinogenesis. Rats were killed 6, 12, 24 and 48 h after IQ administration. The levels of adducts, expressed as relative adduct labeling values in rats treated with beta-C, alpha-T and Aloe, were decreased as compared with the control group at hour 24 (36 h after PH), with a significant difference in the case of the beta-C group (46.4% of the control value). Similarly, all showed a tendency for decrease at hour 48. Furthermore, the levels of CYP1A2, known to be responsible for activation of IQ, showed a significant reduction at hour 24. It is concluded that beta-C, and possibly also alpha-T and Aloe, have the potential to reduce IQ-DNA adduct formation, presumably as a result of decreased formation of active metabolites. The results may explain, at least in part, the previously observed inhibitory effects of these compounds on induction of preneoplastic hepatocellular lesions.

AN: 96217790

MEDLINE EXPRESS (R) 1996 255 of 324

TI: NGF regulatory role in stress and coping of rodents and humans.

AU: Alleva-E; Petruzzi-S; Cirulli-F; Aloe-L

AD: Section of Behavioral Pathophysiology, Istituto Superiore di Sanitaa, Rome, Italy. alleva@iss.it

SO: Pharmacol-Biochem-Behav. 1996 May; 54(1): 65-72

This title is owned by this library

ISSN: 0091-3057

LA: ENGLISH

AB: Nerve growth factor (NGF) is a polypeptide growth factor which exerts trophic and differentiative effects on specific peripheral and central populations of neurons. Recent data showing that various cellular types of the endocrine and immune systems are able to synthesize and release NGF have suggested that this neurotrophic factor may also play an important role in vertebrate physiologic homeostasis. Previous studies using a mouse model of aggressive behavior have shown that NGF levels increase in both plasma and the CNS following intermale agonistic encounters. More recently, we have extended this research area to include other species: in particular, humans. The data now available indicate that labour and lactation, or the occurrence of a stressful event such as the very first jump with a parachute causes in NGF plasma levels as well as changes in the distribution of NGF receptors on lymphocytes. This review aimed to outline the current understanding of NGF role in vertebrates in stress-related events.

AN: 96292565

MEDLINE EXPRESS (R) 1996 256 of 324

TI: Antiinflammatory C-glucosyl chromone from Aloe barbadensis.

AU: Hutter-JA; Salman-M; Stavinoha-WB; Satsangi-N; Williams-RF; Streeper-RT; Weintraub-ST

AD: Department of Pharmacology, Research Imaging Center, University of Texas Health Science Center at San Antonio, 78284-7760, USA.

SO: J-Nat-Prod. 1996 May; 59(5): 541-3

This title is owned by this library

ISSN: 0163-3864

LA: ENGLISH

AB: A new antiinflammatory agent identified as 8-[C-beta-D-[2-O-(E)-cinnamoyl]glucopyranosyl]-2- [(R)-2-hydroxypropyl]-7-methoxy-5-methylchromone (1) has been isolated from Aloe barbadensis Miller. At a dose of 200 microg/mouse ear, 1 exhibited topical antiinflammatory activity equivalent to 200 microg/ear of hydrocortisone. There was no reduction in thymus weight caused by treatment with 1 for any of the doses tested, while 200 microg/ear of hydrocortisone resulted in a 50% decrease in thymus weight.

AN: 96366693

MEDLINE EXPRESS (R) 1996 257 of 324

TI: Management of psoriasis with Aloe vera extract in a hydrophilic cream: a placebo-controlled, double-blind study.

AU: Syed-TA; Ahmad-SA; Holt-AH; Ahmad-SA; Ahmad-SH; Afzal-M

AD: Department of Clinical Physiology, Malmo University Hospital, Sweden.

SO: Trop-Med-Int-Health. 1996 Aug; 1(4): 505-9

This title is owned by this library

ISSN: 1360-2276

LA: ENGLISH

AB: The purpose of this double-blind, placebo-controlled study was to evaluate the clinical efficacy and tolerability of topical Aloe vera extract 0.5% in a hydrophilic cream to cure patients with psoriasis vulgaris. Sixty patients (36M/24F) aged 18-50 years (mean 25.6) with slight to moderate chronic plaque-type psoriasis and PASI (Psoriasis Area and Severity Index) scores between 4.8 and 16.7 (mean 9.3) were enrolled and randomized to two parallel groups. The mean duration of the disease prior to enrollment was 8.5 years (range 1-21). Patients were provided with a precoded 100g tube, placebo or active (with 0.5% Aloe vera extract), and they self-administered trial medication topically (without occlusion) at home 3 times daily for 5 consecutive days per week (maximum 4 weeks active treatment). Patients were examined on a weekly basis and those showing a progressive reduction of lesions, desquamation followed by decreased erythema, infiltration and lowered PASI score were considered healed. The study was scheduled for 16 weeks with 12 months of follow-up on a monthly basis. The treatment was well tolerated by all the patients, with no adverse drug-related symptoms and no dropouts. By the end of the study, the Aloe vera extract cream had cured 25/30 patients (83.3%) compared to the placebo cure rate of 2/30 (6.6%) (P < 0.001) resulting in significant clearing of the psoriatic plaques (328/396 (82.8%) vs placebo 28/366 (7.7%), P < 0.001) and a decreased PASI score to a mean of 2.2. The findings of this study suggest that topically applied Aloe vera extract 0.5% in a hydrophilic cream is more effective than placebo, and has not shown toxic or any other objective side-effects. Therefore, the regimen can be considered a safe and alternative treatment to cure patients suffering from psoriasis.

AN: 96343964

MEDLINE EXPRESS (R) 1996 258 of 324

TI: Non-conventional topical therapies for wound care.

AU: Rund-CR

SO: Ostomy-Wound-Manage. 1996 Jun; 42(5): 18-20, 22-4, 26

ISSN: 0889-5899

LA: ENGLISH

AB: There seems to be a resurgence of interest among healthcare professionals in treating wounds with various topical agents. There are practitioners who are recommending and/or using topical therapies for wounds without having an understanding of why or how they affect a wound. Many of these products are used based upon here-say or clinical observations, since there is a lack of researched-based practice regarding the use of such topicals in wound healing. This article reviews such non-conventional topical therapies as aloe vera, antacids, benzoyl peroxide, collagen, dilantin, gentian violet, impregnated gauzes, insulin, mercurochrome, oxygen therapy, sugar, and vinegar. Each agent is described, its mode of action is discussed, and clinical studies regarding the use of these topicals to promote wound healing are examined, if any. The reader should review this article along with other existing literature regarding topical therapies before making a decision about using such in the care of wounds. When caring for wounds, one should ask, what is the goal of wound care at this particular time? And then ask, based upon available research, what, if any, of these agents can help achieve this goal? The following information should assist one in answering these questions.

AN: 96359011

MEDLINE EXPRESS (R) 1996 259 of 324

TI: Serum NGF levels increase during lactation and following maternal aggression in mice.

AU: Alleva-E; Aloe-L; Cirulli-F; Della-Seta-D; Tirassa-P

AD: Section of Behavioral Pathophysiology, Laboratorio di Fisiopatologia di Organo e di Sistema, Istituto Superiore di Sanita, Rome, Italy.

SO: Physiol-Behav. 1996 Mar; 59(3): 461-6

This title is owned by this library

ISSN: 0031-9384

LA: ENGLISH

AB: In the present study, serum levels of nerve growth factor (NGF) were assessed in virgin and in lactating female CD-1 mice. In the case of the lactating females, NGF levels were assessed both under basal conditions and 60 and 180 min following a 10 min encounter with a male or a nonlactating female mouse. Basal serum NGF levels of lactating females were higher than those of virgin females but did not increase significantly above base after an aggressive encounter with a male or a female conspecific. Female intruders were attacked in a ritualized manner. In contrast, males received numerous bites to vulnerable regions of their body. A positive correlation was found between serum NGF levels and pattern of aggression in females confronting male conspecifics. Thus, in lactating mice, serum NGF levels following an aggressive encounter relate to the specific pattern of behavior the female uses to defend the offspring.

AN: 96231486

MEDLINE EXPRESS (R) 1996 260 of 324

TI: Hypoallergenic products [letter]

AU: Gale-AE

SO: Med-J-Aust. 1996 Jul 1; 165(1): 62

This title is owned by this library

ISSN: 0025-729X

LA: ENGLISH

AN: 96292152

MEDLINE EXPRESS (R) 1996 261 of 324

TI: Schistosoma mansoni: influence of infection on mouse behavior.

AU: Fiore-M; Moroni-R; Alleva-E; Aloe-L

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: Exp-Parasitol. 1996 Jun; 83(1): 46-54

This title is owned by this library

ISSN: 0014-4894

LA: ENGLISH

AB: Schistosoma mansoni infection in humans and animals induces abnormal neurobehavioral responses following granuloma formation. In mice, granulomas in the liver are observed 8 weeks after infection, while after 15-20 weeks, the presence of eggs and granulomas in the brain has been reported. In this study, outbred CD-1 female mice were infected with S. mansoni and examined in several behavioral tests (open field, novel object investigation, black/white box, and hot plate) 8 and 15 weeks after infection. The detected effects of schistosome infection were a reduction of body weight in 8-week infected mice, marked changes in exploration/activity, rearing, and wall-rearing in 8- and 15-week infected mice, an enhancement of sniffing and grooming in 8-week infected mice, and finally an increase in the threshold of pain response to the hot plate in 15-week infected mice. The results of the present study indicate that S. mansoni infection markedly alters exploratory behavior of mice, affecting particularly the vertical movements of the animals, and suggests that the differences in behavioral abnormalities between 8- and 15-week infected mice might be associated with modifications in the levels of nerve growth factor and cytokines induced by granulomas.

AN: 96267108

MEDLINE EXPRESS (R) 1996 262 of 324

TI: The preventive and therapeutic potential of the squalene-containing compound, Roidex, on tumor promotion and regression.

AU: Desai-KN; Wei-H; Lamartiniere-CA

AD: Department of Pharmacology and Toxicology, University of Alabama at Birmingham, 35294, USA.

SO: Cancer-Lett. 1996 Mar 19; 101(1): 93-6

This title is owned by this library

ISSN: 0304-3835

LA: ENGLISH

AB: Recent scientific evidence has shown free radicals or reactive oxygen species (ROS) to play an important role in the initiation and progression of cancer. Many radical scavengers have also been found to help reduce the attacks by these ROS. Interestingly, the ROS scavengers that have been investigated are naturally occurring compounds such as vitamins C and E. Roidex is a formulation of squalene, vitamin e, and aloe vera. It was our goal to investigate whether Roidex was able to prevent the development of chemically induced cancer and to cause regression of any tumors already formed in a mouse skin model. In the prevention study, skin tumors were initiated in 50 female CD-1 mice with 7,12-dimethylbenz[a]-anthracene (DMBA) and promoted with 12-O-tetradecanoylphorbol-13-acetate (TPA). The mice were treated with either mineral oil, 5% squalene, or Roidex. At the end of the prevention study, there was a 33.34% incidence to tumors (multiplicity of 1.40) in the mineral oil-treatment group, 26.67% (multiplicity of 0.467) in the 5% squalene and Roidex groups, respectively. The tumor regression study involved the selection of mice with tumors and possible regression of these tumors with Roidex treatment. There was a regression of 33.34% of the tumors in the Roidex-treated group (39 tumors to 26 tumors) compared to the non-treated group whose tumors regressed only 3.44% (29 tumors to 28 tumors).

AN: 96202642

MEDLINE EXPRESS (R) 1996 263 of 324

TI: Nerve growth factor is an autocrine survival factor for memory B lymphocytes.

AU: Torcia-M; Bracci-Laudiero-L; Lucibello-M; Nencioni-L; Labardi-D; Rubartelli-A; Cozzolino-F; Aloe-L; Garaci-E

AD: Department of Experimental Medicine, University of Rome "Tor Vergata", Italy.

SO: Cell. 1996 May 3; 85(3): 345-56

This title is owned by this library

ISSN: 0092-8674

LA: ENGLISH

AB: Production of nerve growth factor (NGF) was assessed in cultures of human T and B lymphocytes and macrophages. NGF was constitutively produced by B cells only, which also expressed surface p140trk-A and p75NGFR molecules and hence efficiently bound and internalized the cytokine. Neutralization of endogenous NGF caused disappearance of Bcl-2 protein and apoptotic death of resting lymphocytes bearing surface IgG or IgA, a population comprising memory cells, while surface IgM/IgD "virgin" B lymphocytes were not affected. In vivo administration of neutralizing anti-NGF antibodies caused strong reduction in the titer of specific IgG in mice immunized with tetanus toxoid, nitrophenol, or arsonate and reduced numbers of surface IgG or IgA B lymphocytes. Thus, NGF is an autocrine survival factor for memory B lymphocytes.

AN: 96201524

MEDLINE EXPRESS (R) 1996 264 of 324

TI: Intracerebral injection of human immunodeficiency virus type 1 coat protein gp120 differentially affects the expression of nerve growth factor and nitric oxide synthase in the hippocampus of rat.

AU: Bagetta-G; Corasaniti-MT; Aloe-L; Berliocchi-L; Costa-N; Finazzi-Agro-A; Nistico-G

AD: Department of Biology, Mondino-Tor Vergata Center for Experimental Neurobiology, Rome, Italy.

SO: Proc-Natl-Acad-Sci-U-S-A. 1996 Jan 23; 93(2): 928-33

This title is owned by this library

ISSN: 0027-8424

LA: ENGLISH

AB: We have studied the neuropathological characteristics of the brain of rats receiving daily intracerebroventricular administration of freshly dissolved human immunodeficiency virus type 1 recombinant protein gp120 (100 ng per rat per day) given for up to 14 days. Histological examination of serial brain sections revealed no apparent gross damage to the cortex or hippocampus, nor did cell counting yield significant neuronal cell loss. However, the viral protein caused after 7 and 14 days of treatment DNA fragmentation in 10% of brain cortical neurons. Interestingly, reduced neuronal nitric oxide synthase (NOS) expression along with significant increases in nerve growth factor (NGF) were observed in the hippocampus, where gp120 did not cause neuronal damage. No changes in NGF and NOS expression were seen in the cortex, where cell death is likely to be of the apoptotic type. The present data demonstrate that gp120-induced cortical cell death is associated with the lack of increase of NGF in the cerebral cortex and suggest that the latter may be important for the expression of neuropathology in the rat brain. By contrast, enhanced levels of NGF may prevent or delay neuronal death in the hippocampus, where reduced NOS expression may be a reflection of a subcellular insult inflicted by the viral protein.

AN: 96149412

MEDLINE EXPRESS (R) 1997 265 of 324

TI: An overview of the topical management of wounds.

AU: Liptak-JM

AD: Department of Companion Animal Medicine and Surgery, University of Queensland.

SO: Aust-Vet-J. 1997 Jun; 75(6): 408-13

ISSN: 0005-0423

LA: ENGLISH

AB: Wounds in animals are a common and frequent reason for seeking veterinary attention. The way in which wounds are managed affect the rate of healing, the time to return to normal function, the final cosmetic appearance, and hence the satisfaction of customers. The management of wounds depends on the stage of wound healing and can include irrigation, mechanical and chemical debridement, the use of antiseptics and antimicrobials, adherent and nonadherent dressings, and miscellaneous topical applications such as aloe vera, honey and live yeast cell derivative. The advantages, disadvantages and indications for initial wound management, topical applicants and dressings are discussed.

AN: 97390853

MEDLINE EXPRESS (R) 1997 266 of 324

TI: The expanding role of nerve growth factor: from neurotrophic activity to immunologic diseases.

AU: Aloe-L; Bracci-Laudiero-L; Bonini-S; Manni-L

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: Allergy. 1997 Sep; 52(9): 883-94

This title is owned by this library

ISSN: 0105-4538

LA: ENGLISH

AB: Numerous studies published in the last 10-15 years have shown that nerve growth factor (NGF), a polypeptide originally discovered in connection with its neurotrophic activity, also acts on cells of the immune system. NGF has been found in various immune organs including the spleen, lymph nodes, and thymus, and cells such as mast cells, eosinophils, and B and T cells. The circulating levels of NGF increase in inflammatory responses, in various autoimmune diseases, in parasitic infections, and in allergic diseases. Stress-related events both in animal models and in man also result in an increase of NGF, suggesting that this molecule is involved in neuroendocrine functions. The rapid release of NGF is part of an alerting signal in response to either psychologically stressful or anxiogenic conditions in response to homeostatic alteration. Thus, the inflammation and stress-induced increase in NGF might alone or in association with other biologic mediators induce the activation of immune cells during immunologic insults. A clearer understanding of the role of NGF in these events may be useful to identify the mechanisms implicated in certain neuroimmune and immune dysfunctions.

AN: 97443311

MEDLINE EXPRESS (R) 1997 267 of 324

TI: Acrokeratoelastoidosis: a report of two sporadic cases [letter]

AU: Rubegni-P; De-Aloe-G; Romano-C; Flori-ML; Fimiani-M

SO: Clin-Exp-Dermatol. 1997 Jan; 22(1): 62-4

This title is owned by this library

ISSN: 0307-6938

LA: ENGLISH

AN: 97471125

MEDLINE EXPRESS (R) 1997 268 of 324

TI: Complete Melkersson-Rosenthal syndrome in a patient with Crohn's disease [letter]

AU: De-Aloe-G; Rubegni-P; Mazzatenta-C; Fimiani-M

SO: Dermatology. 1997; 195(2): 182

This title is owned by this library

ISSN: 1018-8665

LA: ENGLISH

AN: 97456777

MEDLINE EXPRESS (R) 1997 269 of 324

TI: Prenatal exposure to ethanol causes differential effects in nerve growth factor and its receptor in the basal forebrain of preweaning and adult rats.

AU: Angelucci-F; Cimino-M; Balduini-W; Piltillo-L; Aloe-L

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: J-Neural-Transplant-Plast. 1997 Mar-Jun; 6(2): 63-71

This title is owned by this library

ISSN: 0792-8483

LA: ENGLISH

AB: In this study we investigated nerve growth factor (NGF) levels in the cortex and hippocampus of the offspring of pregnant female Sprague-Dawley rats receiving a single intragastric administration of acute ethanol on the 15th day of gestation and compared them with a control group of rats that received an injection of sucrose. We also examined the distribution of the low-affinity NGF receptor, p75NGFR, on NGF-responsive neurons that are localized in the septum and the nucleus of Meynert, which receive the respective trophic support from the hippocampus and the cortex. In the ethanol-treated group, the results show that at post-natal age 15 days, the NGF septohippocampal pathways were markedly affected. At day 15, the NGF level was significantly higher in the offspring of ethanol-treated rats. By day 40, NGF values in both groups decreased to similar levels. At day 60, however, the NGF level in the ethanol-treated animals decreased to a significantly lower value than that of the control group, which remained essentially unchanged. In parallel, at day 60 the numbers of septal cholinergic neurons expressing p75NGFR were also significantly lower in ethanol-treated rats than in control animals. Because ethanol is known to induce neurological disorders, as well as deficits in cell proliferation and differentiation, the results suggest that one cause of the deleterious effects induced by ethanol is the low availability of NGF during certain stages of postnatal brain development.

AN: 97451262

MEDLINE EXPRESS (R) 1997 270 of 324

TI: Nerve growth factor and autoimmune rheumatic diseases.

AU: Aloe-L; Tuveri-MA

AD: Institute of Neurobiology, Rome, Italy.

SO: Clin-Exp-Rheumatol. 1997 Jul-Aug; 15(4): 433-8

This title is owned by this library

ISSN: 0392-856X

LA: ENGLISH

AB: Nerve growth factor (NGF) is the first discovered and best known neurotrophic factor and is required for the survival and differentiation of a variety of neuronal cell types in both the peripheral and central nervous system. Recent studies indicate that NGF is synthesized by cells of immune system lineage and that its level increases during inflammatory responses, while cytokines such as interleukin-1 beta and tumor necrosis factor-alpha are potent inducers of NGF secretion. The role played by NGF on cells of the immune system was strengthened by recent evidence demonstrating that cells normally present in inflammatory tissues, such as mast cells and lymphocytes, express NGF receptors and are receptive to the action of NGF. Studies carried out in our and other laboratories showed that NGF is expressed in the synovial fluid of patients with rheumatoid arthritis and other forms of chronic arthritis, as well as in the synovium of pharmacologically-induced arthritis in animal models. Moreover, arthritic transgenic mice which carry and express the human tumor necrosis factor-gene also showed elevated levels of NGF. Significant increases in NGF levels have been found in the sera of patients with systemic lupus erythematosus and in the dermis of patients affected by systemic sclerosis. In this paper the hypothesis that NGF is involved in the pathophysiology of autoimmune rheumatic arthritis is discussed.

AN: 97418302

MEDLINE EXPRESS (R) 1997 271 of 324

TI: Inhibition of benzo[a]pyrene-DNA adduct formation by Aloe barbadensis Miller.

AU: Kim-HS; Lee-BM

AD: Division of Toxicology, College of Pharmacy, Sung Kyun Kwan University, Suwon City, Kyunggi-Do, South Korea.

SO: Carcinogenesis. 1997 Apr; 18(4): 771-6

This title is owned by this library

ISSN: 0143-3334

LA: ENGLISH

AB: The antigenotoxic and chemopreventive effect of Aloe barbadensis Miller (polysaccharide fraction) on benzo[a]pyrene (B[a]P)-DNA adducts was investigated in vitro and in vivo. Aloe showed a time-course and dose-dependent inhibition of [3H]B[a]P-DNA adduct formation in primary rat hepatocytes (1x10(6) cells/ml) treated with [3H]B[a]P (4 nmol/ml). At concentrations of 0.4-250 microg/ml aloe, the binding of [3H]B[a]P metabolites to rat hepatocyte DNA was inhibited by 9.1-47.9%. Also, in rat hepatocytes cultured for 3-48 h with aloe (250 microg/ml) and [3H]B[a]P (4 nmol/ml), [3H]B[a]P-DNA adducts were significantly reduced by 36% compared with [3H]B[a]P alone. Aloe also inhibited cellular uptake of [3H]B[a]P in a dose-dependent manner at a concentration of 0.4-250 microg/ml by 6.3-34.1%. After a single oral administration of B[a]P to male ICR mice (10 mg/mouse), benzo[a]pyrene diol epoxide I (BPDE-I)-DNA adduct formation and persistence for 16 days following daily treatment with aloe (50 mg/mouse) were quantitated by enzyme-linked immunosorbent assay using monoclonal antibody 8E11. In this animal model, BPDE-I-DNA adduct formation was significantly inhibited in various organs (liver, kidney, forestomach and lung) (P < 0.001). When mice were pretreated with aloe for 16 days before B[a]P treatment, inhibition of BPDE-I-DNA adduct formation and persistence was enhanced. Glutathione S-transferase activity was slightly increased in the liver but cytochrome P450 content was not affected by aloe. These results suggest that the inhibitory effect of aloe on BPDE-I-DNA adduct formation might have a chemopreventive effect by inhibition of B[a]P absorption.

AN: 97265276

MEDLINE EXPRESS (R) 1997 272 of 324

TI: Nerve growth factor effects on the song control system of zebra finches.

AU: Fiore-M; Patel-SN; Alleva-E; Aloe-L; Clayton-NS

AD: Division of Biological Sciences, University of California, Davis 95616, USA.

SO: Neurosci-Lett. 1997 Feb 28; 223(3): 161-4

This title is owned by this library

ISSN: 0304-3940

LA: ENGLISH

AB: The aim of this experiment was to test whether or not nerve growth factor (NGF) is involved in cholinergic processes in the avian brain, by injecting NGF into the higher vocal center (HVC) and examining its effects on adult male zebra finch song. Since NGF has been hypothesized to protect cells after injury, some birds received both NGF and ibotenic acid (IBO) lesions of HVC, while others received either NGF or IBO or neither (SHAM). Only the IBO-treated birds showed alterations in song. Although there was no evidence of cell preservation in the immunocytochemical and morphological analysis NGF appears to prevent the IBO induced impairment in song augmenting the activity of the remaining neurons and enhancing brain repair.

AN: 97235334

MEDLINE EXPRESS (R) 1997 273 of 324

TI: Human CD4+ T cell clones produce and release nerve growth factor and express high-affinity nerve growth factor receptors.

AU: Lambiase-A; Bracci-Laudiero-L; Bonini-S; Bonini-S; Starace-G; D'Elios-MM; De-Carli-M; Aloe-L

AD: Institute of Neurobiology, Consiglio Nazionale Richerche, Department of Ophthalmology, University of Rome Tor Vergata, Italy.

SO: J-Allergy-Clin-Immunol. 1997 Sep; 100(3): 408-14

This title is owned by this library

ISSN: 0091-6749

LA: ENGLISH

AB: BACKGROUND: Increasing evidence shows that nerve growth factor (NGF) plays a role in the complex and fascinating linkage between the nervous and the immune systems due to its ability to modulate functions of several inflammatory cells. OBJECTIVE: To investigate NGF receptor expression and NGF production and release by human CD4+ cells clones, which have primary relevance in modulating inflammatory events through their different subsets of functional phenotypes. METHODS: The expression of NGF and a transmembrane tyrosine kinase (TrkA) was evaluated by immunohistochemistry and flow cytometry analysis in five T(H0), six T(H1), and five T(H2) cell clones derived from human circulating mononuclear blood cells. Moreover, the amount of NGF protein was assessed by measuring the NGF levels in culture supernatants of the T cell clones before stimulation and 48 hours after phytohemagglutinin (PHA) activation by use of an immunoenzymatic assay. RESULTS: Our data have shown that in unstimulated conditions, human CD4+ T cell clones express both immunoreactivity for NGF and the TrkA NGF receptor irrespective of their cytokine profile. Moreover, T(H1) and T(H2) clones, but not T(H0) clones, secrete NGF in basal conditions. PHA activation induces NGF secretion in T(H0) clones and a significant increase of NGF levels in T(H2) (p < 0.05), but not in T(H1) culture supernatants. CONCLUSIONS: Results obtained represent the first evidence of TrkA expression and NGF production and release in human CD4+ cell clones and suggest a possible functional role of NGF in modulating the immune and inflammatory network.

AN: 97458011

MEDLINE EXPRESS (R) 1997 274 of 324

TI: Increased plasma levels of substance P in vernal keratoconjunctivitis.

AU: Lambiase-A; Bonini-S; Micera-A; Tirassa-P; Magrini-L; Bonini-S; Aloe-L

AD: Institute of Neurobiology, National Research Council, and Department of Ophthalmology, University of Rome Tor Vergata, Italy.

SO: Invest-Ophthalmol-Vis-Sci. 1997 Sep; 38(10): 2161-4

This title is owned by this library

ISSN: 0146-0404

LA: ENGLISH

AB: PURPOSE: The increase of nerve growth factor (NGF) plasma levels in vernal keratoconjunctivitis (VKC) patients has been demonstrated previously. Results of numerous studies in vitro and in vivo have shown that NGF modulates the synthesis of substance P (SP), a neuropeptide involved in the pathogenesis of human allergic diseases. In this study the involvement of SP in this allergic conjunctivitis is investigated, along with its relationship with NGF and other systemic and local markers of VKC. METHODS: Competitive radioimmunoassays were used to detect the levels of SP in plasma, the levels of eosinophil cationic protein, and the total and specific immunoglobulin E in the serum of 11 patients with VKC and in 11 healthy matched controls. Plasma levels of nerve growth factor (NGF) were measured in all VKC patients and controls using an immunoenzymatic assay. Histologic evaluation was performed in tarsal and bulbar conjunctival specimens obtained in biopsies from 8 VKC patients and 4 control subjects. RESULTS: Patients with VKC show a significant increase of SP and NGF plasma levels (P < 0.003 and P < 0.001, respectively), and an increase of eosinophil cationic protein and immunoglobulin E levels in the serum (P < 0.001 and P < 0.002, respectively). Mast cells, eosinophils, and lymphocytes were also significantly increased in the conjunctiva of VKC patients. Interestingly enough, VKC patients with the highest NGF plasma levels also showed the highest SP levels. CONCLUSIONS: The data show the involvement of SP in VKC and suggest that SP with NGF could modulate the allergic response in this disease, probably through an interaction with inflammatory cytokines.

AN: 97470820

MEDLINE EXPRESS (R) 1997 275 of 324

TI: Upregulation of phagocytosis and candidicidal activity of macrophages exposed to the immunostimulant acemannan.

AU: Stuart-RW; Lefkowitz-DL; Lincoln-JA; Howard-K; Gelderman-MP; Lefkowitz-SS

AD: Department of Biological Sciences, Texas Tech University, Lubbock 79409, U.S.A.

SO: Int-J-Immunopharmacol. 1997 Feb; 19(2): 75-82

ISSN: 0192-0561

LA: ENGLISH

AB: Previous studies by these investigators have shown that mannosylated bovine serum albumin (m-BSA) enhances the respiratory burst (RB), phagocytosis, and killing of Candida albicans by resident murine peritoneal macrophages (MO). Upregulation of the above MO functions was associated with binding of m-BSA to the MO-mannose receptor. The present study was done to determine if the immunostimulant, acemannan prepared from aloe vera, could stimulate MO in a similar manner. Resident peritoneal MO collected from C57BL/6 mice were exposed to acemannan for 10 min. The RB was measured using chemiluminescence and demonstrated approximately a two-fold increase above the media controls. In studies involving phagocytosis, MO were exposed to acemannan, washed and exposed to Candida at a ratio of 1:5. The percent phagocytosis and Candida killing were determined using fluorescence microscopy. There was a marked increase in phagocytosis in the treated cultures (45%) compared to controls (25%). Macrophages exposed to acemannan for 10 min resulted in ca 38% killing of Candida albicans compared with 0-5% killing in controls. If MO were incubated with acemannan for 60 min, 98% of the yeast were killed compared to 0-5% in the controls. The results of the present study indicate that short term exposure of MO to acemannan upregulates the RB, phagocytosis and candidicidal activity. Further studies are needed to clarify the potential use of this immunostimulant as an anti-fungal agent.

AN: 97422409

MEDLINE EXPRESS (R) 1997 276 of 324

TI: Haloperidol administration in humans lowers plasma nerve growth factor level: evidence that sedation induces opposite effects to arousal.

AU: Aloe-L; Iannitelli-A; Bersani-G; Alleva-E; Angelucci-F; Maselli-P; Manni-L

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: Neuropsychobiology. 1997; 36(2): 65-8

This title is owned by this library

ISSN: 0302-282X

LA: ENGLISH

AB: Studies reported in recent years have indicated that the level of nerve growth factor (NGF), in both the brain and in the bloodstream, increases following stressful events and anxiety-associated behaviour. These observations prompted us to investigate whether an anti-arousal drug would induce an opposite effect. We have reported that the administration of haloperidol (HA), a neuroleptic drug clinically used for psychiatric disorders, decreases NGF levels in the hypothalamus of adult male mice. In the present study, we showed that HA reduced the basal NGF plasma levels in 8 neuroleptic-free schizophrenic patients. These observations strengthen the hypothesis that NGF may play a functional role in stress-coping responses.

AN: 97413211

MEDLINE EXPRESS (R) 1997 277 of 324

TI: Removal of the submaxillary salivary glands and infection with the trematode Schistosoma mansoni alters exploratory behavior and pain thresholds in female mice.

AU: Fiore-M; Moroni-R; Aloe-L

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: Physiol-Behav. 1997 Aug; 62(2): 399-406

This title is owned by this library

ISSN: 0031-9384

LA: ENGLISH

AB: In this study, CD-1 female mice, deprived of the submaxillary salivary glands, were infected with S. mansoni and their behavior was observed 15 weeks after infection, when the eggs of the parasite are present in the brain. Sialectomized infected mice showed changes in exploratory activity, sniffing, and wall-rearing in the open-field and in the black/white box, but no differences in pain sensitivity were observed on the hot plate. The present results suggest that the modifications in the behavior of sialectomized infected mice might be associated with the inability of the animals to cope with the aversive effects of the infection and, most probably, with modifications in the levels of polypeptides released into the bloodstream by the salivary glands, affecting the NGF-responsive cells of the nervous, endocrine, and immune systems.

AN: 97394268

MEDLINE EXPRESS (R) 1997 278 of 324

TI: Paclitaxel neurotoxicity: clinical and neurophysiological study of 23 patients.

AU: Pace-A; Bove-L; Aloe-A; Nardi-M; Pietrangeli-A; Calabresi-F; Innocenti-P; Jandolo-B

AD: Servizio di Neurologia, Istituto Nazionale Regina Elena per lo studio e la cura dei Tumori, Roma, Italy.

SO: Ital-J-Neurol-Sci. 1997 Apr; 18(2): 73-9

This title is owned by this library

ISSN: 0392-0461

LA: ENGLISH

AB: Paclitaxel is the prototype of a new class of chemotherapeutic agents with an antimitotic effect that is related to its ability to interfere with the microtubule system. It causes peripheral neurological toxicity by means of its activity on the axonal microtubules. To define the clinical and neurophysiological characteristics of paclitaxel neuropathy 23 patients undergoing paclitaxel therapy at a dose of 175 mg/m2 were studied. The patients were divided into two groups, with only one group receiving pretreatment with potentially neurotoxic drugs such as cisplatin and carboplatin. The results showed a high incidence of mild neurotoxicity in both groups. Treatment was discontinued due to severe neurotoxicity in only one patient pretreated with platinum-compounds. The clinical and neurophysiological data make it possible to define paclitaxel neurotoxicity as a distal axonal neuropathy with a summatory effect in patients pretreated with cisplatin; the possible reversibility of paclitaxel neurotoxicity requires further confirmation.

AN: 97383534

MEDLINE EXPRESS (R) 1997 279 of 324

TI: Necrotizing fasciitis: case study of a nursing dilemma.

AU: Ardire-L

AD: Shore Memorial Hospital, Somers Point, NJ, USA.

SO: Ostomy-Wound-Manage. 1997 Jun; 43(5): 30-4, 36, 38-40; passim

ISSN: 0889-5899

LA: ENGLISH

AB: Necrotizing fasciitis usually manifests as a low grade cellulitis that quickly deteriorates to a limb and life threatening soft tissue infection. Immediate surgical debridement is essential, after which wound management becomes the nurse's primary concern. Case #1 reports on a 72 year old female who, upon presenting to the ER with a "sore bottom," subsequently had these diagnoses: (1) anal-rectal abscess, (2) Fournier's gangrene, (3) ulcerative enterocolitis, (4) chronic blood loss/anemia, and (5) protein caloric malnutrition. After debridement, her anal-rectal wound extended from labia to left buttocks. Care was multidisciplinary and included applying a water based aloe gel and saline soaked gauze twice a day. After 45 days, the wound exhibited a pink base with granulation tissue and contraction of the wound edges. Case #2 reports on a 48 year old male with seroma of the left leg secondary to a crush injury. Within three days he developed deep vein thrombosis in that leg as well as two large seroma cavities on either side of the thigh. Care included packing with the aloe gel and saline soaked sponges. Two weeks after admission, the anterior wound was covered with a split thickness skin graft while partial closure of the lateral cavity was attempted unsuccessfully with retention sutures. After five weeks, healing was complete for the anterior wound and 95 percent complete for the posterior wound.

AN: 97377580

MEDLINE EXPRESS (R) 1997 280 of 324

TI: Paclitaxel as salvage therapy in advanced pretreated ovarian cancer: a phase II study.

AU: Nardi-M; Aloe-A; De-Marco-S; Cognetti-F; Iacovelli-A; Atlante-G; Calabresi-F

AD: Regina Elena Cancer Institute, Rome, Italy.

SO: Am-J-Clin-Oncol. 1997 Jun; 20(3): 230-2

ISSN: 0277-3732

LA: ENGLISH

AB: The response rate to salvage chemotherapy in advanced ovarian cancer has been disappointing in patients who do not respond or who relapse after platinum-containing regimens. In these cases, the identification of new drugs is a substantial challenge. The efficacy of one of these, paclitaxel, has already been assessed in many phase II trials. From July 1993 to October 1995, 33 patients with advanced ovarian cancer, recurrent or refractory after platinum-based regimens, entered our study. Paclitaxel was given by 3-hour intravenous infusion every 3 weeks. All the patients were evaluable for toxicity and 27 for response. Nine patients (33.3%) responded: 6 complete (22.2%) and 3 partial responses (11.1%). Six responses (35.3%) were observed in the 17 platinum-resistant patients and 3 (30%) in the 10 platinum-responders. World Health Organization (WHO) grade 3-4 neutropenia was common (13/33 patients, 39.4%) and peripheral neurotoxicity was observed in 29 patients (87.8%), but was WHO grade 3 in four cases (12.1%). Alopecia was ubiquitous, whereas other toxic effects were not significant. The overall response rate to paclitaxel in this study is similar to that reported in others and the high complete response (CR) rate should be emphasized. These data confirm the significant activity and safety of this drug in patients with advanced ovarian cancer, even in platinum-resistant cases.

AN: 97310575

MEDLINE EXPRESS (R) 1997 281 of 324

TI: The use of genomic in situ hybridization (GISH) to show transmission of recombinant chromosomes by a partially fertile bigeneric hybrid, Gasteria lutzii x Aloe aristata (Aloaceae), to its progeny.

AU: Takahashi-C; Leitch-IJ; Ryan-A; Bennett-MD; Brandham-PE

AD: Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, UK.

SO: Chromosoma. 1997 Apr; 105(6): 342-8

This title is owned by this library

ISSN: 0009-5915

LA: ENGLISH

AB: Genomic in situ hybridization (GISH) was used to study somatic chromosomes of parental and progeny plants (all 2n=2x=14) of the bigeneric hybrid between Gasteria lutzii and Aloe aristata (Aloaceae), which is partially fertile, a rare occurrence in plants. GISH successfully distinguished between the two parental genomes in the F1 hybrid and revealed numerous genomic recombinations in chromosomes transmitted by the F1 to the back-cross progeny. The results indicate high levels of meiotic compatibility between the parental genomes, even though they differ in size by 20%. Recombination occurred at a frequency that was higher than that expected from the analysis of orcein-stained meiosis in the F1. The discrepancy suggests that terminalization may occur prior to or during metaphase I, reducing the apparent chiasma frequency, or possibly reveals an under-estimation caused by difficulties in resolving closely grouped chiasmata by eye.

AN: 97244572

MEDLINE EXPRESS (R) 1997 282 of 324

TI: Extracorporeal photochemotherapy induces a significant increase in CD36+ circulating monocytes in patients with mycosis fungoides.

AU: Fimiani-M; Rubegni-P; Pimpinelli-N; Mori-M; De-Aloe-G; Andreassi-L

AD: Department of Dermatology, University of Siena, Italy.

SO: Dermatology. 1997; 194(2): 107-10

This title is owned by this library

ISSN: 1018-8665

LA: ENGLISH

AB: BACKGROUND: In patients with Sezary syndrome undergoing extracorporeal photochemotherapy it is currently thought that photodamage of a congruent number of pathogenic circulating T cells is a necessary condition for a specific anticlonal response to be induced against these cells by the immune system. However other mechanisms of action, such as the induction of release by photoirradiated monocytes of some cytokines, are thought to cooperate in the induction of a therapeutic response. OBJECTIVE: We investigated the effects of extracorporeal photochemotherapy on peripheral blood mononuclear cells in seven stage Ib cutaneous T cell lymphoma patients. METHOD: Samples of peripheral blood were taken before starting extracorporeal photochemotherapy (baseline), and before the 3rd and 6th cycle of treatment, and then incubated with specific monoclonal antibodies. RESULTS: The results showed a significant increase of CD8+ and CD36+ and a significant decrease of CD25+ in all the treated patients. CONCLUSION: Our results show that extracorporeal photochemotherapy is able to induce an increase of CD36+ cell in peripheral blood of patients with mycosis fungoides in its early stages. This subset of monocytes could be involved in the complex mechanism of action of extracorporeal photochemotherapy.

AN: 97248228

MEDLINE EXPRESS (R) 1997 283 of 324

TI: Aloesin up-regulates cyclin E/CDK2 kinase activity via inducing the protein levels of cyclin E, CDK2, and CDC25A in SK-HEP-1 cells.

AU: Lee-KY; Park-JH; Chung-MH; Park-YI; Kim-KW; Lee-YJ; Lee-SK

AD: College of Pharmacy, Seoul National University, Korea.

SO: Biochem-Mol-Biol-Int. 1997 Feb; 41(2): 285-92

This title is owned by this library

ISSN: 1039-9712

LA: ENGLISH

AB: In the present study, we show that aloesin, which is a low molecular weight ingredients present in Aloe vera, stimulates the proliferation of cultured human hepatoma SK-HEP-1 cells. The incorporation of [3H] thymidine into DNA in the cell cultures was significantly increased at a dose of 10 microM aloesin. The aloesin-induced DNA synthesis appears to require newly synthesized proteins because cycloheximide treatment blocked the DNA synthesis evoked by this compound. We then examined whether this compound increases the intracellular levels of cell cycle regulators by immunoblotting. The data showed that aloesin increased the levels of cyclin E, CDK2, and CDC25A in SK-HEP-1 cells. In addition, immuno-complex kinase assays showed that aloesin up-regulated the enzyme activity of cyclin E/CDK2 kinase in a dose-dependent manner. Collectively, these results suggest that aloesin stimulates the proliferation of SK-HEP-1 cells by inducing the intracellular levels of cyclin E/CDK2 kinase complex and CDC25A, which, together, result in the up-regulation of cyclin E-dependent kinase activity.

AN: 97217597

MEDLINE EXPRESS (R) 1997 284 of 324

TI: Time-course changes of nerve growth factor, corticotropin-releasing hormone, and nitric oxide synthase isoforms and their possible role in the development of inflammatory response in experimental allergic encephalomyelitis.

AU: Calza-L; Giardino-L; Pozza-M; Micera-A; Aloe-L

AD: Institute of Human Physiology, University of Cagliari, Italy. cefisnmo@mbox.vol.it

SO: Proc-Natl-Acad-Sci-U-S-A. 1997 Apr 1; 94(7): 3368-73

This title is owned by this library

ISSN: 0027-8424

LA: ENGLISH

AB: In this paper we report a time-course study of development of experimental allergic encephalomyelitis in Lewis rats, by monitoring neuroendocrine regulation of the hypothalamus-pituitary-adrenal axis through corticotropin-releasing hormone mRNA expression, inflammatory cellular infiltrate, macrophagic and neuronal nitric oxide synthase, nerve growth factor (NGF), and NGF p75 and trkA receptors in the brain and spinal cord. We analyzed animals during 20 days after immunization, a time interval that corresponds to the acute immunological phase. We have described a severe, early fall of corticotropin-releasing hormone mRNA expression, which could account for the decreased response of the hypothalamus-pituitary-adrenal axis to inflammatory stress. During this period, an increase of neuronal nitric oxide synthase was observed in the cerebral cortex and spinal cord, and macrophagic nitric oxide synthase positive cells were found in the inflammatory cellular infiltrate, which was abundant in perivascular and submeningeal areas 20 days after immunization. Concomitantly, we found a dramatic up-regulation of NGF receptors on the wall of blood vessels and adjacent neurons in perivascular areas. NGF content also had increased in some brain areas, such as the thalamus, while it had decreased in others, like the spinal cord and medulla oblongata, at time points in which the most serious cellular infiltrate was found.

AN: 97250544

MEDLINE EXPRESS (R) 1997 285 of 324

TI: The synergistic purgative action of aloe-emodin anthrone and rhein anthrone in mice: synergism in large intestinal propulsion and water secretion.

AU: Yagi-T; Yamauchi-K; Kuwano-S

AD: Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan.

SO: J-Pharm-Pharmacol. 1997 Jan; 49(1): 22-5

This title is owned by this library

ISSN: 0022-3573

LA: ENGLISH

AB: This study aimed to explore the mechanism involved in the synergistic purgative action of aloe-emodin anthrone and rhein anthrone, the active metabolites of sennoside C. Aloe-emodin anthrone and rhein anthrone, and their equimolar mixture, induced excretion of an approximately equal number of faeces by intracaecal administration at a dose of 23.2 mumol kg-1 in mice (= 1.0 standard dose). The number of wet faeces induced by aloe-emodin anthrone was less than those of rhein anthrone and the mixture. At the same dose, rhein anthrone and the mixture significantly stimulated large intestinal propulsion, though aloe-emodin anthrone had little stimulatory effect. Aloe-emodin anthrone and rhein anthrone decreased net water absorption but could not reverse it to the net secretion at 1/2 dose. The mixture significantly decreased net water absorption and reversed it to the net secretion at this dose. These anthrones did not stimulate mucus secretion in the colon at 1/2 dose. We concluded that the synergistic purgative effect of aloe-emodin anthrone and rhein anthrone in mice results from synergistic stimulation of large intestinal transit and large intestinal water secretion.

AN: 97176171

MEDLINE EXPRESS (R) 1997 286 of 324

TI: High-dose anabolic androgenic steroids modulate concentrations of nerve growth factor and expression of its low affinity receptor (p75-NGFr) in male rat brain.

AU: Tirassa-P; Thiblin-I; Agren-G; Vigneti-E; Aloe-L; Stenfors-C

AD: Institute of Neurobiology, C.N.R., Rome, Italy.

SO: J-Neurosci-Res. 1997 Jan 15; 47(2): 198-207

This title is owned by this library

ISSN: 0360-4012

LA: ENGLISH

AB: The effects of treatment with a high dose of nandrolone or testosterone on nerve growth factor (NGF) levels and NGF low-affinity receptor (p75-NGFr) distribution in the brain were analyzed. Nandrolone, subcutaneously injected in rats for several weeks, caused an increase of NGF levels in the hippocampus and septum and a decrease in the hypothalamus. The number of p75-NGFr-immunoreactive neurons and the p75-NGFr expression levels were reduced in the septum and vertical and horizontal Broca's bands. Testosterone injections caused an increase of NGF levels in the hippocampus, septum, and occipital cortex and induced an upregulation of p75-NGFr in the forebrain NGF target regions. This testosterone effect suggests that nandrolone and testosterone affect brain NGF target cells by a different mechanism(s). Nandrolone may interfere with NGF transport and/or utilization by forebrain neurons, causing an altered p75-NGFr expression and NGF accumulation as a consequence. Since NGF is known to maintain forebrain neurons and to regulate neurobehavioral functions, including memory, learning, and defensive behavior, it is possible to hypothesize that this neurotrophin may play a role in the mechanism of action of anabolic androgenic steroids (AAS) in the brain and be associated with endocrine and behavioral dysfunctions occurring due to AAS abuse.

AN: 97160830

MEDLINE EXPRESS (R) 1997 287 of 324

TI: Isolation and characterization of the glycoprotein fraction with a proliferation-promoting activity on human and hamster cells in vitro from Aloe vera gel.

AU: Yagi-A; Egusa-T; Arase-M; Tanabe-M; Tsuji-H

AD: Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima, Japan.

SO: Planta-Med. 1997 Feb; 63(1): 18-21

This title is owned by this library

ISSN: 0032-0943

LA: ENGLISH

AB: Fractions of leaf gel from Aloe barbadensis Mill. were prepared by gel permeation using DEAE Sephadex A-25, Sepharose 6B, and Sephadex G-50 columns. These were then tested by in vitro assays for proliferation of human normal dermal and baby hamster kidney cells. The glycoprotein fraction promoted cell growth, while the neutral polysaccharide fraction did not show any growth stimulation. Moreover, the polar-colored glycoprotein fraction strongly inhibited the in vitro assays. An active glycoprotein fraction (protein 82%, carbohydrate 11%) examined on polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE showed a single band. Its molecular weight was 29 kD on a Sephadex G-50 column and its isoelectric point was pH 6.8. Immunoblotting after SDS-PAGE showed that the glycoprotein was composed of two subunits (14 kD). Deglycosylation of glycoprotein (Pg21-2b fraction) by trifluoromethanesulphonic acid provided a protein band with a molecular weight of 13 kD on SDS-PAGE. The colored glycoprotein fraction was shown on SDS-PAGE to be a mixture with a molecular weight of 18 kD-15 kD. It was later hydrolyzed with 10% H2SO4 to produce phenolic substances.

AN: 97217070

MEDLINE EXPRESS (R) 1997 288 of 324

TI: Thyroid hormone regulates NGF content and p75LNGFR expression in the basal forebrain of adult rats.

AU: Calza-L; Giardino-L; Aloe-L

AD: Pathophysiology Center for the Nervous System, University of Cagliari, Modena, Italy.

SO: Exp-Neurol. 1997 Feb; 143(2): 196-206

This title is owned by this library

ISSN: 0014-4886

LA: ENGLISH

AB: Several lines of data from human and animal studies have suggested a role of thyroid hormone in the regulation of cholinergic neurons in the adult brain. In this study we have investigated the content of nerve growth factor (NGF) and the expression of NGF low affinity receptor (p75(LNGFR)) in the basal forebrain of adult hypothyroid rats. We describe an increase of both NGF and p75(LNGFR) expression in the basal forebrain of adult hypothyroid rats. The administration of colchicine up-regulates p75(LNGFR) expression in both hypo- and control rats, whereas it fails to down-regulate choline acetyl transferase mRNA expression during hypothyroidism. These data offer a possible neurobiological explanation to cognitive defects observed during adult hypothyroidism in humans.

AN: 97209597

MEDLINE EXPRESS (R) 1997 289 of 324

TI: Treatment of Pemphigus senilis with tetracycline plus nicotinamide: long term follow-up [letter]

AU: Fimiani-M; Rubegni-P; Flori-ML; De-Aloe-G; Andreassi-L

SO: J-Am-Geriatr-Soc. 1997 Feb; 45(2): 256-8

This title is owned by this library

ISSN: 0002-8614

LA: ENGLISH

AN: 97185858

MEDLINE EXPRESS (R) 1997 290 of 324

TI: Assessment of the genotoxic risk from laxative senna products.

AU: Brusick-D; Mengs-U

AD: Corning Hazleton Inc., Vienna, Virginia 22182, USA.

SO: Environ-Mol-Mutagen. 1997; 29(1): 1-9

This title is owned by this library

ISSN: 0893-6692

LA: ENGLISH

AB: Laxative senna products and several of their specific components have been submitted to a large number of genetic tests. While most studies gave negative responses, results from some of the studies suggest that components of senna products, particularly emodin and aloe-emodin, have genotoxic activity. Assessment of the genotoxicity profile of these substances, in light of other data from animal and human metabolism or kinetic studies, human clinical trials and rodent carcinogenicity studies do not support concerns that senna laxatives pose a genotoxic risk to humans when consumed under prescribed use conditions.

AN: 97172217

MEDLINE EXPRESS (R) 1998/01-1998/10 291 of 324

TI: Development of systemic lupus erythematosus in mice is associated with alteration of neuropeptide concentrations in inflamed kidneys and immunoregulatory organs.

AU: Bracci-Laudiero-L; Aloe-L; Stenfors-C; Theodorsson-E; Lundeberg-T

AD: Karolinska Institutet, Stockholm, Sweden. luisa@biocell.irmkant.rm.cnr.it

SO: Neurosci-Lett. 1998 May 29; 248(2): 97-100

This title is owned by this library

ISSN: 0304-3940

LA: ENGLISH

AB: In the present study we used a well-characterised model of murine lupus, the female NZB/W hybrid, to study the possible involvement of neuropeptides in the pathogenesis of systemic lupus erythematosus (SLE). Analysis of neuropeptides with a possible role in inflammation showed that substance P (SP) calcitonin gene-related peptide (CGRP) and neuropeptide Y (NPY) are present in increased quantities in the inflamed kidneys of SLE mice, confirming their involvement in local inflammation, while there is a general reduction in the peptide concentrations in the lymphoid organs of lupus mice, except for NPY. Our results suggest that the altered neuropeptide concentrations observed in the SLE lymphoid organs may be partly responsible for the altered immune response and contribute to the development of autoimmune diseases.

AN: 98316923

MEDLINE EXPRESS (R) 1998/01-1998/10 292 of 324

TI: The effects of nutritional supplements on the symptoms of fibromyalgia and chronic fatigue syndrome.

AU: Dykman-KD; Tone-C; Ford-C; Dykman-RA

AD: Mannatech Inc., Coppell Texas 75019, USA.

SO: Integr-Physiol-Behav-Sci. 1998 Jan-Mar; 33(1): 61-71

ISSN: 1053-881X

LA: ENGLISH

AB: This article reports the results of a within-subject design. Fifty subjects with a physician diagnosis of fibromyalgia (FM) and/or chronic fatigue syndrome (CFS) were interviewed using a structured interview from. Each subject was interviewed initially, and again nine months later (follow-up). Subjects had, on their own, consumed nutritional supplements including freeze-dried aloe vera gel extract; a combination of freeze-dried aloe vera gel extract and additional plant-derived saccharides; freeze-dried fruits and vegetables in combination with the saccharides; and a formulation of dioscorea complex containing the saccharides and a vitamin/mineral complex. With medical treatments, approximately 25 percent of FM patients improve, but the beneficial effects of medical treatment rarely persist more than a few months. All subjects in this study had received some form of medical treatment prior to taking the nutritional supplements, but none with enduring success. Nutritional supplements resulted in a remarkable reduction in initial symptom severity, with continued improvement in the period between initial assessment and the follow-up. Further research is needed to verify these results, specifically crossover designs in well-defined populations.

AN: 98256712

MEDLINE EXPRESS (R) 1998/01-1998/10 293 of 324

TI: Early maternal separation increases NGF expression in the developing rat hippocampus.

AU: Cirulli-F; Micera-A; Alleva-E; Aloe-L

AD: Section of Behavioral Pathophysiology, Laboratorio di Fisiopatologia di Organo e di Sistema, Istituto Superiore di Sanita, Rome, Italy.

SO: Pharmacol-Biochem-Behav. 1998 Apr; 59(4): 853-8

This title is owned by this library

ISSN: 0091-3057

LA: ENGLISH

AB: Nerve Growth Factor (NGF) is a neurotrophin involved in growth and differentiation of central cholinergic neurons. In this study a maternal separation paradigm was used to test whether levels of NGF might be affected by brief manipulations of rat pups early during ontogeny. The expression of NGF mRNA was examined in 3-day-old rat pups following 45 min maternal separation using in situ hybridization. Early maternal separation in neonatal rats resulted in increased expression of NGF mRNA in the dentate gyrus and the hilus of the hippocampus. NGF protein levels measured (by means of a sensitive ELISA assay) in the whole hippocampus the day following the separation procedure did not differ in separated vs. nonseparated pups. These data indicate that brief manipulations performed early during development can affect hippocampal NGF expression.

AN: 98246253

MEDLINE EXPRESS (R) 1998/01-1998/10 294 of 324

TI: Biotransformation of the anthraquinones emodin and chrysophanol by cytochrome P450 enzymes. Bioactivation to genotoxic metabolites.

AU: Mueller-SO; Stopper-H; Dekant-W

AD: Department of Toxicology, University of Wurzburg, Versbacherstr. 9, 97078 Wurzburg, Germany.

SO: Drug-Metab-Dispos. 1998 Jun; 26(6): 540-6

This title is owned by this library

ISSN: 0090-9556

LA: ENGLISH

AB: The studies presented here were designed to elucidate the enzymes involved in the biotransformation of naturally occurring 1, 8-dihydroxyanthraquinones and to investigate whether biotransformation of 1,8-dihydroxyanthraquinones may represent a bioactivation pathway. We first studied the metabolism of emodin (1, 3,8-trihydroxy-6-methylanthraquinone), a compound present in pharmaceutical preparations. With rat liver microsomes, the formation of two emodin metabolites, omega-hydroxyemodin and 2-hydroxyemodin, was observed. The rates of formation of omega-hydroxyemodin were not different with microsomes from rats that had been pretreated with inducers for different cytochrome P450 enzymes. Thus, the formation of omega-hydroxyemodin seems to be catalyzed by several cytochrome P450 enzymes at low rates. The formation of 2-hydroxyemodin was increased in liver microsomes from 3-methylcholanthrene-pretreated rats and was inhibited by alpha-naphthoflavone, by an anti-rat cytochrome P450 1A1/2 antibody, and, to a lesser degree, by an anti-rat cytochrome P450 1A1 antibody. These data suggest the involvement of cytochrome P450 1A2 in the formation of this metabolite. However, other cytochrome P450 enzymes also seem to catalyze this reaction. The anthraquinone chrysophanol (1,8-dihydroxy-3-methylanthraquinone) is transformed, in a cytochrome P450-dependent oxidation, to aloe-emodin (1, 8-dihydroxy-3-hydroxymethylanthraquinone) as the major product formed. The mutagenicity of the parent dihydroxyanthraquinones and their metabolites was compared in the in vitro micronucleus test in mouse lymphoma L5178Y cells. 2-Hydroxyemodin induced much higher micronucleus frequencies, compared with emodin. omega-Hydroxyemodin induced lower micronucleus frequencies, compared with emodin. Aloe-emodin induced significantly higher micronucleus frequencies than did chrysophanol. These data indicate that the cytochrome P450-dependent biotransformation of emodin and chrysophanol may represent bioactivation pathways for these compounds.

AN: 98282253

MEDLINE EXPRESS (R) 1998/01-1998/10 295 of 324

TI: Immunochemical distinction of Aloe vera, A. arborescens, and A. chinensis gels [letter]

AU: Yagi-A; Tsunoda-M; Egusa-T; Akasaki-K; Tsuji-H

SO: Planta-Med. 1998 Apr; 64(3): 277-8

This title is owned by this library

ISSN: 0032-0943

LA: ENGLISH

AB: Verectin antiserum raised in white rabbits was immunoprecipitated with the Aloe vera nondialysable fraction. Analysis of the immunoprecipitation revealed that verectin accounted for about 1.25% of the total proteins in the nondialysable fraction of Aloe vera gel. The verectin antibody showed differential immunoreactivities against nondialysable fractions of A. arborescens, A. chinensis, and A. vera: 1) an immunopreciptin line was formed against the fraction of A. vera, but not against those of A. arborescens and A. chinensis gel in an Ouchterlony double immunodiffusion test and 2) an immunopositive band was detected in the A. vera and A. chinensis nondialysable fractions but not in that of A. arborescens in immunoblotting. These findings indicate that the verectin antibody can be used to distinguish Aloe materials.

AN: 98242628

MEDLINE EXPRESS (R) 1998/01-1998/10 296 of 324

TI: Levels of expression of hRPB11, a core subassembly subunit of human RNA polymerase II, affect doxorubicin sensitivity and cellular differentiation.

AU: Bruno-T; Leonetti-C; Aloe-S; Iacobini-C; Floridi-A; Di-Tondo-U; Punturieri-A; Fanciulli-M

AD: Cell Metabolism and Pharmacokinetics Laboratory, Regina Elena Cancer Institute, Rome, Italy.

SO: FEBS-Lett. 1998 May 8; 427(2): 241-6

This title is owned by this library

ISSN: 0014-5793

LA: ENGLISH

AB: We have previously shown that the human RNA polymerase II subunit 11 (hRPB11) is among the proteins specifically downregulated upon Doxorubicin (Dox) treatment of human cancer cell lines, and that Dox resistant clones derived upon drug selection express about 20% of the protein present in the original parental cell line. Given the prominent role that this subunit appears to have in eukaryotic cells, and the fact that its deletion causes lethality in yeast, we wanted to test the effect of the reintroduction of parental cell line levels of this subunit in Dox resistant colon cancer cells (LoVoDX). Stable transfectants of LoVoDX expressing parental (LoVoH) levels of hRPB11 showed a reduced sensitivity to the drug without changing the response of these cells to other chemotherapeutic agents, confirming a specific inverse correlation between cellular Dox sensitivity anti-hRPB11 levels of expression. In addition we show here that the levels of expression of this same RNA polymerase II subunit directly affect cellular differentiation, reducing the rate of cell proliferation, clonogenicity and increasing the expression of E-cadherin, a marker of epithelial cell differentiation. As expected from cells with these characteristics, upon in vivo administration of these clones in nude mice, we detected a significant reduction in the size and time of appearance of the primary tumors and overall metastatic capability. Finally, the role played by hRPB11 in regulating the transcription of specific genes is underlined by transient transfection experiments that show transactivation of the E-cadherin promoter by this protein.

AN: 98268335

MEDLINE EXPRESS (R) 1998/01-1998/10 297 of 324

TI: Expression of nerve growth factor receptors on the ocular surface in healthy subjects and during manifestation of inflammatory diseases.

AU: Lambiase-A; Bonini-S; Micera-A; Rama-P; Bonini-S; Aloe-L

AD: Institute of Neurobiology, National Research Council, Rome, Italy.

SO: Invest-Ophthalmol-Vis-Sci. 1998 Jun; 39(7): 1272-5

This title is owned by this library

ISSN: 0146-0404

LA: ENGLISH

AB: PURPOSE: Recent studies have suggested the involvement of nerve growth factor (NGF) in the conjunctival inflammatory process and in corneal epithelium proliferation and differentiation. To verify the hypothesis that NGF could locally modulate the inflammatory and reparative processes, the authors evaluated the expression of NGF high-affinity receptor on the ocular surface in normal and pathologic conditions. METHODS: Ten conjunctival biopsies (obtained from three healthy subjects, five patients affected by vernal keratoconjunctivitis [VKC], and two patients with cicatricial pemphigoid [CP]) and five corneal specimens obtained from the Eye Bank of Veneto (Italy) were evaluated. All specimens were histologically stained, and immunohistochemistry was performed to identify the NGF high-affinity receptor (TrkA). RESULTS: All tissues expressed immunoreactivity for NGF receptors. In conjunctival specimens of healthy subjects, basal epithelial cells strongly expressed immunoreactivity and, in the stroma, rare cells were immunopositive for TrkA. No significant difference in immunoreactivity was observed in the conjunctival epithelium between healthy subjects and patients with inflammatory conjunctival diseases, whereas there were more immunopositive cells observed in the conjunctival stroma of VKC and CP patients than in the controls. The immunoreactivity in the cornea was confined to basal epithelial cells and endothelium. CONCLUSIONS: The NGF receptor is present on the human ocular surface. The authors' data support the possibility that NGF modulates ocular inflammation and corneal epithelial proliferation and differentiation through its receptors.

AN: 98281400

MEDLINE EXPRESS (R) 1998/01-1998/10 298 of 324

TI: Influence of Aloe vera on collagen characteristics in healing dermal wounds in rats.

AU: Chithra-P; Sajithlal-GB; Chandrakasan-G

AD: Department of Biochemistry, Central Leather Research Institute, Adyar, Madras, India.

SO: Mol-Cell-Biochem. 1998 Apr; 181(1-2): 71-6

This title is owned by this library

ISSN: 0300-8177

LA: ENGLISH

AB: Wound healing is a fundamental response to tissue injury that results in restoration of tissue integrity. This end is achieved mainly by the synthesis of the connective tissue matrix. Collagen is the major protein of the extracellular matrix, and is the component which ultimately contributes to wound strength. In this work, we report the influence of Aloe vera on the collagen content and its characteristics in a healing wound. It was observed that Aloe vera increased the collagen content of the granulation tissue as well as its degree of crosslinking as seen by increased aldehyde content and decreased acid solubility. The type I/type III collagen ratio of treated groups were lower than that of the untreated controls, indicating enhanced levels of type III collagen. Wounds were treated either by topical application or oral administration of Aloe vera to rats and both treatments were found to result in similar effects.

AN: 98220712

MEDLINE EXPRESS (R) 1998/01-1998/10 299 of 324

TI: Aloe barbadensis extracts reduce the production of interleukin-10 after exposure to ultraviolet radiation.

AU: Byeon-SW; Pelley-RP; Ullrich-SE; Waller-TA; Bucana-CD; Strickland-FM

AD: Department of Immunology, The University of Texas MD Anderson Cancer Center, Houston 77030, USA.

SO: J-Invest-Dermatol. 1998 May; 110(5): 811-7

This title is owned by this library

ISSN: 0022-202X

LA: ENGLISH

AB: Cutaneous exposure to ultraviolet radiation suppresses the induction of T cell mediated responses such as contact and delayed type hypersensitivity (DTH) by altering the function of immune cells in the skin and causing the release of immunoregulatory cytokines. Extracts of crude Aloe barbadensis gel prevent this photosuppression. Because the regulation of contact hypersensitivity and DTH responses differ, we investigated whether protection was afforded by a single or multiple agents in Aloe and the mechanism by which this material prevents suppression of DTH immunity. The ability of Aloe gel to prevent suppression of contact hypersensitivity responses to hapten decayed rapidly after manufacture. In contrast, agents that protected against systemic suppression of DTH responses to Candida albicans were stable over time. Oligosaccharides prepared from purified Aloe polysaccharide prevented suppression of DTH responses in vivo and reduced the amount of IL-10 observed in ultraviolet irradiated murine epidermis. To assess the effect of Aloe extracts on keratinocytes, Pam 212 cells were exposed in vitro to ultraviolet radiation and treated for 1 h with Aloe oligosaccharides. Culture supernatants were collected 24 h later and injected into mice. Supernatants from ultraviolet irradiated keratinocytes suppressed the induction of DTH responses, whereas Aloe oligosaccharide treatment reduced IL-10 and blocked the suppressive activity of the supernatants. These results indicate that Aloe contains multiple immunoprotective factors and that Aloe oligosaccharides may prevent ultraviolet induced suppression of DTH by reducing keratinocyte derived immunosuppressive cytokines.

AN: 98239154

MEDLINE EXPRESS (R) 1998/01-1998/10 300 of 324

TI: Cholecystokinin-8 regulation of NGF concentrations in adult mouse brain through a mechanism involving CCK(A) and CCK(B) receptors.

AU: Tirassa-P; Stenfors-C; Lundeberg-T; Aloe-L

AD: Institute of Neurobiology (CNR), Rome, Italy.

SO: Br-J-Pharmacol. 1998 Mar; 123(6): 1230-6

This title is owned by this library

ISSN: 0007-1188

LA: ENGLISH

AB: 1. Nerve growth factor (NGF), a powerful agent for the growth, differentiation and regeneration of lesioned cells of the central and peripheral nervous systems, has in recent years been indicated as a potential therapeutic agent capable of reversing the processes of cell damage in neurodegenerative events in man. Since NGF does not cross the blood-brain barrier and central NGF administration requires invasive surgical procedures, the discovery of substances modulating in vivo NGF synthesis in the brain will be extremely useful for a possible clinical use of NGF. 2. The aim of the present study to analyse if the content of NGF in the brain of adult mice can be affected by peripheral administration of cholecystokinin-8 (CCK-8), a well known neuropeptide which has stimulant actions on neurons in the brain and promotes a variety of neurobehavioural effects both in man and rodents. 3. The dose-response and time course effects of an i.p. injection of CCK-8 on the NGF concentrations in the hippocampus, cortex, hypothalamus and pituitary of adult male mice were analysed by use of a sensitive immunoenzymatic assay for NGF. The effects of pretreatment with selective CCK(A) and CCK(B) receptor antagonists and atropine on the NGF response to CCK injection were also studied. 4. The effects of CCK-8 were dose- and time-dependent and the injection of 8 nmol kg(-1) resulted in a 3 fold increase of NGF levels in the hypothalamus and pituitary, and about a 60% increase in the hippocampus. No effects were observed in the cortex. Pretreatment with a selective CCK(A) receptor antagonist blocked the CCK-induced NGF increase in the hypothalamus and pituitary. In the hippocampus the same effect was obtained with a CCK(B) receptor antagonist. Pretreatment with atropine suppressed the CCK-induced effects on NGF levels in all the brain regions examined. 5. Our results showing that i.p. injection with CCK-8 can modulate NGF levels in the brain through a mechanism which seems, in part, to be mediated via the vagal afferents, indicate that this neuropeptide may represent a useful pharmacological approach to enhance endogenous NGF levels in neuropathologies associated with a neurotrophin deficit.

AN: 98218836

MEDLINE EXPRESS (R) 1998/01-1998/10 301 of 324

TI: Exploratory and displacement behavior in transgenic mice expressing high levels of brain TNF-alpha.

AU: Fiore-M; Alleva-E; Probert-L; Kollias-G; Angelucci-F; Aloe-L

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: Physiol-Behav. 1998 Feb 15; 63(4): 571-6

This title is owned by this library

ISSN: 0031-9384

LA: ENGLISH

AB: Studies reported recently have shown that tumor necrosis factor-alpha (TNF-alpha) a cytokine released by macrophages and monocytes plays a key role in inflammatory processes and immune and neuro-endocrine regulation. TNF-alpha is also produced in the central nervous system (CNS). However, the role of this cytokine in the CNS is largely unknown, although evidence indicates that it is involved in various neurobehavioral manifestations. Using transgenic mice expressing high amounts of murine TNF-alpha transgene in the neurons of the CNS, we investigated the stereotyped, exploratory, and displacement activities in the hole-board and black/white box. Transgenic mice and their normal control littermates were hybrids of the CBA x C57BL/6 genetic backgrounds and were obtained by backcrossing the CBA x C57BL/6 founder female and her progeny with F1 hybrid mates. Transgenic mice did not show changes in the stereotyped behavior on the hole-board, but they displayed several alterations in the exploratory activities both in the hole-board and black/white box. Transgenic mice also exhibited an increase in grooming when exposed to a highly unfamiliar environmental stimuli in the black/white box. The study suggests that supranormal endogenous TNF-alpha in the brain affects the behavioral responses to stressful conditions.

AN: 98182074

MEDLINE EXPRESS (R) 1998/01-1998/10 302 of 324

TI: TNF-alpha expressed in the brain of transgenic mice lowers central tyroxine hydroxylase immunoreactivity and alters grooming behavior.

AU: Aloe-L; Fiore-M

AD: Institute of Neurobiology, CNR, Rome, Italy. aloe@biocell.irmkant.rm.cnr.it

SO: Neurosci-Lett. 1997 Nov 28; 238(1-2): 65-8

This title is owned by this library

ISSN: 0304-3940

LA: ENGLISH

AB: Tumor necrosis factor-alpha (TNF-alpha) is a cytokine involved in a wide range of biological effects both in physiological and non-physiological conditions. It is also produced in the central nervous system (CNS) where it has been implicated in reparative processes after traumatic injuries and in CNS demyelination, neurodegeneration and inflammation. Using transgenic mice (Tg-m) expressing TNF-alpha specifically in the CNS, we showed that the overexpression of this cytokine reduced tyroxine hydroxylase immunoreactivity (TH-ir) in the caudate-putamen and in the dorsomedial hypothalamic areas and impaired grooming behavior. We also showed that this behavior is increased following anti-nerve growth factor injection. These findings support the hypothesis, proposed by others, that TNF-alpha is involved in the degenerative processes which occur in Parkinson's disease.

AN: 98124303

MEDLINE EXPRESS (R) 1998/01-1998/10 303 of 324

TI: Isolation of cDNA for an NADP-malic enzyme from Aloe arborescens.

AU: Honda-H; Shimada-H; Akagi-H

AD: Life Science Institute, Mitsui Chemicals, Inc., Mobara, Japan.

SO: DNA-Res. 1997 Dec 31; 4(6): 397-400

ISSN: 1340-2838

LA: ENGLISH

AB: NADP-malic enzyme catalyzes the reaction of decarboxylation from malate. In CAM plants, functions of this enzyme diverged to include both photosynthetic and non-photosynthetic roles. A full length cDNA for an NADP-malic enzyme was isolated from an 'obligate' CAM plant aloe (Aloe arborescens). The cDNA contains an ORF encoding 592 amino acid residues, whose sequence is highly homologous to the known plant NADP-malic enzymes. This gene is constitutively expressed in all organs in a low level. The amount of the transcript exhibited no diurnal variation, suggesting that this gene is not involved in photosynthetic functions.

AN: 98162727

MEDLINE EXPRESS (R) 1998/01-1998/10 304 of 324

TI: Aloe-emodin effects on arylamine N-acetyltransferase activity in the bacterium Helicobacter pylori [letter]

AU: Wang-HH; Chung-JG; Ho-CC; Wu-LT; Chang-SH

SO: Planta-Med. 1998 Mar; 64(2): 176-8

This title is owned by this library

ISSN: 0032-0943

LA: ENGLISH

AB: Arylamine N-acetyltransferase (NAT) activities with p-aminobenzoic acid (PABA) and 2-aminofluorene (AF) were determined in H. pylori collected from peptic ulcer patients. Cytosols or suspensions of H. pylori with or without different concentrations of aloe-emodin co-treatment showed different percentages of AF and PABA acetylation. The data indicate that there was decreased NAT activity associated with increased aloe-emodin in H. pylori cytosols. Inhibition of growth study from H. pylori demonstrated that aloe-emodin elicited dose-dependent growth inhibition in H. pylori cultures. The report is the first finding of aloe-emodin inhibition of arylamine NAT activity in a strain of H. pylori.

AN: 98185824

MEDLINE EXPRESS (R) 1998/01-1998/10 305 of 324

TI: Topical treatment with nerve growth factor for corneal neurotrophic ulcers [see comments]

AU: Lambiase-A; Rama-P; Bonini-S; Caprioglio-G; Aloe-L

AD: Department of Ophthalmology, University of Rome Tor Vergata, Italy.

SO: N-Engl-J-Med. 1998 Apr 23; 338(17): 1174-80

This title is owned by this library

ISSN: 0028-4793

LA: ENGLISH

AB: BACKGROUND: Corneal neurotrophic ulcers associated with impairment of sensory innervation of the cornea may lead to loss of vision, and there is no effective treatment for these ulcers. We evaluated the effects of nerve growth factor in patients with this disorder. METHODS: Twelve patients (14 eyes) with severe neurotrophic corneal ulcers associated with corneal anesthesia were treated with topical nerve growth factor 10 times daily for two days and then 6 times daily until the ulcers healed. Treatment continued for 2 weeks after the ulcers healed, and the patients were then followed for up to 12 months. The evolution of the corneal disease during treatment and follow-up was evaluated by slit-lamp examination, photography, fluorescein-dye testing, and tests of corneal sensitivity and best corrected visual acuity. RESULTS: Corneal healing began 2 to 14 days after the initiation of treatment with nerve growth factor, and all patients had complete healing of their corneal ulcers after 10 days to 6 weeks of treatment. Corneal sensitivity improved in 13 eyes, and returned to normal in 2 of the 13 eyes. Corneal integrity and sensitivity were maintained during the follow-up period (range, 3 to 12 months). Best corrected visual acuity increased progressively during treatment and follow-up in all patients. There were no systemic or local side effects of treatment. CONCLUSIONS: In this preliminary, uncontrolled study, topically applied exogenous nerve growth factor restored corneal integrity in patients with corneal neurotrophic ulcers.

AN: 98209521

MEDLINE EXPRESS (R) 1998/01-1998/10 306 of 324

TI: Proliferation and phenotype regulation in the subventricular zone during experimental allergic encephalomyelitis: in vivo evidence of a role for nerve growth factor.

AU: Calza-L; Giardino-L; Pozza-M; Bettelli-C; Micera-A; Aloe-L

AD: Department of Biochemistry and Human Physiology, University of Cagliari, 09124 Cagliari, Italy. cefisnmo@mbox.vol.it

SO: Proc-Natl-Acad-Sci-U-S-A. 1998 Mar 17; 95(6): 3209-14

This title is owned by this library

ISSN: 0027-8424

LA: ENGLISH

AB: Proliferating cells in the subventricular zone (SVZ) of adult rat brain could provide a source of cells for repair attempts during degenerative diseases. However, very few reports dealt with the spontaneous regulation of this cell population during experimental conditions. In this paper, we describe an increase in the proliferation activity in the SVZ during experimental allergic encephalomyelitis, a demyelinating disease widely used as an experimental model for human multiple sclerosis. Moreover, p75(LNGFR)-immunoreactive elements in the SVZ were larger in experimental allergic encephalomyelitis compared with control groups, and they also showed multiple and branched elongations. Finally, a selective uptake of 125I-nerve growth factor was observed in the SVZ in neonatal rats, and positive elements migrated in the corpus callosum within a few days. These data indicate that cell populations in the SVZ are regulated during inflammatory conditions and degenerative diseases involving oligodendrocytes and neurotrophins, including nerve growth factor, could participate in these phenomena.

AN: 98169523

MEDLINE EXPRESS (R) 1998/01-1998/10 307 of 324

TI: Influence of aloe vera on the healing of dermal wounds in diabetic rats.

AU: Chithra-P; Sajithlal-GB; Chandrakasan-G

AD: Department of Biochemistry, Central Leather Research Institute, Adyar, Chennai, India.

SO: J-Ethnopharmacol. 1998 Jan; 59(3): 195-201

This title is owned by this library

ISSN: 0378-8741

LA: ENGLISH

AB: The positive influence of Aloe vera, a tropical cactus, on the healing of full-thickness wounds in diabetic rats is reported. Full-thickness excision/incision wounds were created on the back of rats, and treated either by topical application on the wound surface or by oral administration of the Aloe vera gel to the rat. Wound granulation tissues were removed on various days and the collagen, hexosamine, total protein and DNA contents were determined, in addition to the rates of wound contraction and period of epithelialization. Measurements of tensile strength were made on treated/untreated incision wounds. The results indicated that Aloe vera treatment of wounds in diabetic rats may enhance the process of wound healing by influencing phases such as inflammation, fibroplasia, collagen synthesis and maturation, and wound contraction. These effects may be due to the reported hypoglycemic effects of the aloe gel.

AN: 98167322

MEDLINE EXPRESS (R) 1998/01-1998/10 308 of 324

TI: Influence of Aloe vera on the glycosaminoglycans in the matrix of healing dermal wounds in rats.

AU: Chithra-P; Sajithlal-GB; Chandrakasan-G

AD: Department of Biochemistry, Central Leather Research Institute, Madras, India.

SO: J-Ethnopharmacol. 1998 Jan; 59(3): 179-86

This title is owned by this library

ISSN: 0378-8741

LA: ENGLISH

AB: The influence of Aloe vera (L.) Burman f. on the glycosaminoglycan (GAG) components of the matrix in a healing wound was studied. Wound healing is a dynamic and complex sequence of events of which the major one is the synthesis of extracellular matrix components. The early stage of wound healing is characterized by the laying down of a provisional matrix, which is then followed by the formation of granulation tissue and synthesis of collagen and elastin. The provisional matrix or the ground substance consists of GAGs and proteoglycans (PGs), which are protein GAG conjugates. In the present work, we have studied the influence of Aloe vera on the content of GAG and its types in the granulation tissue of healing wounds. We have also reported the levels of a few enzymes involved in matrix metabolism. The amount of ground substance synthesized was found to be higher in the treated wounds, and in particular, hyaluronic acid and dermatan sulphate levels were increased. The levels of the reported glycohydrolases were elevated on treatment with Aloe vera, indicating increased turnover of the matrix. Both topical and oral treatments with Aloe vera were found to have a positive influence on the synthesis of GAGs and thereby beneficially modulate wound healing.

AN: 98167320

MEDLINE EXPRESS (R) 1998/01-1998/10 309 of 324

TI: A role of the thymus and thymosin-alpha1 in brain NGF levels and NGF receptor expression.

AU: Turrini-P; Tirassa-P; Vigneti-E; Aloe-L

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: J-Neuroimmunol. 1998 Feb; 82(1): 64-72

This title is owned by this library

ISSN: 0165-5728

LA: ENGLISH

AB: Using neonatal rats we investigated the role of the thymus and thymosin-alpha1 (T-alpha1) in brain NGF levels, NGF receptor (p75NGFr) expression, as well as the activity of choline acetyl-transferase, a cholinergic enzyme regulated by NGF. It is shown that early postnatal thymectomy causes a decrease in NGF in the hippocampus and cortex and p75NGFr distribution in the basal forebrain cholinergic neurons (FBCN). Intracerebral T-alpha1 injection in thymectomized animals induces a recovery, albeit not complete, of both NGF and p75NGFr. These findings indicate that thymectomy affects both the brain NGF producing and responding cells and that T-alpha1 may be one of the thymic hormones involved in the regulation of cerebral NGF synthesis.

AN: 98186042

MEDLINE EXPRESS (R) 1998/01-1998/10 310 of 324

TI: Presence of nerve growth factor in the thymus of prenatal, postnatal and pregnant rats.

AU: Aloe-L; Micera-A; Bracci-Laudiero-L; Vigneti-E; Turrini-P

AD: Institute of Neurobiology, Rome, Italy.

SO: Thymus. 1997; 24(4): 221-31

This title is owned by this library

ISSN: 0165-6090

LA: ENGLISH

AB: The presence of Nerve Growth Factor (NGF) and the expression of low- and high-affinity NGF receptor were investigated in prenatal, postnatal and pregnant rats. Using ELISA and immunohistochemistry it was found that both NGF and its receptors are present in the medulla of the thymus and are more strongly expressed in pre- and early postnatal life and nearly absent in adult and ageing rats. It was also found that during pregnancy, which is characterised by an involution of the cortex and hypertrophy of the medulla, the level of NGF in the thymus increases. The present study showed not only that NGF is produced in a specific compartment of the thymus, the medulla, but that its synthesis declines with age, following thymus involution. These results suggest that NGF may be critically involved in the proliferation and differentiation of thymic cells.

AN: 98154336

MEDLINE EXPRESS (R) 1998/01-1998/10 311 of 324

TI: Postnatal NGF administration causes adult hyperalgesia and overreactivity to social stimuli but does not reverse capsaicin induced hypoalgesia.

AU: Alleva-E; Aloe-L; Cirulli-F; De-Acetis-L; Padoa-Schioppa-C

AD: Section of Behavioural Pathophysiology, Istituto Superiore di Sanita, Rome, Italy. alleva@iss.it

SO: Psychoneuroendocrinology. 1997 Nov; 22(8): 591-602

This title is owned by this library

ISSN: 0306-4530

LA: ENGLISH

AB: The present longitudinal analysis was aimed at assessing (i) the effects of developmental capsaicin (CAPS) administration on nociceptive responsivity and on the response of adult mice to social stimuli; (ii) the action of NGF on the ontogeny of the same nociceptive response and social stimuli; (iii) whether capsaicin treatment could be reversed by subsequent treatment with NGF. CD-1 mouse pups were treated with either capsaicin (50 mg/kg, s.c.) or vehicle on postnatal days (PNDs) 5 and 8. Every other day from PND 9 to PND 21 the same pups received a daily injection of NGF (0.75 mg/kg, s.c.). During both the prepuberal stage (PNDs 14, 21, and 28) and adulthood, mice were repeatedly tested in a hot-plate apparatus (52 +/- 0.1 degrees C for 1 min). At adulthood they also underwent an aggressive behaviour test. NGF-treated mice showed a shorter latency to hindlimb licking response in the hot plate compared to both controls and NGF-CAPS groups. CAPS-treated subjects showed a long-lasting hypoalgesia at both prepuberal and adult stages that was not modified by subsequent NGF treatment. Finally, NGF-treated mice were more aggressive than both controls and CAPS-NGF animals.

AN: 98144698

MEDLINE EXPRESS (R) 1998/01-1998/10 312 of 324

TI: Anthraquinones in Rheum palmatum and Rumex dentatus (Polygonaceae), and phorbol esters in Jatropha curcas (Euphorbiaceae) with molluscicidal activity against the schistosome vector snails Oncomelania, Biomphalaria and Bulinus.

AU: Liu-SY; Sporer-F; Wink-M; Jourdane-J; Henning-R; Li-YL; Ruppel-A

AD: Institute of Tropical Hygiene and Public Health, University, Heidelburg, Germany.

SO: Trop-Med-Int-Health. 1997 Feb; 2(2): 179-88

This title is owned by this library

ISSN: 1360-2276

LA: ENGLISH

AB: Hot water extracts of Rheum plamatum and Rheum dentatus (from China) showed molluscicidal activity against the snails Oncomelania hupensis, Biomphalaria glabrata and Bulinus globosus, which are vectors of Schistosoma japonicum, S. mansoni and S. haematobium, respectively. Activity was correlated with antraquinones which were identified by HPLC: rhein and chrysophanol-anthron were most active (> 50% dead snails after 2 days in a 0.03% solution). Molluscicidal activity was intermediate with Rheum-emodin and physcion and was not detectable with cinnamic acid or Aloe-emodin. The snail O. hupensis tended to be more sensitive for several compounds than B. glabrata. Extracts of Jatropha curcas seeds (from Mali) showed molluscicidal activity against both B. glabrata and O. hupensis, the latter being the more sensitive snail. The activity was associated with phorbol esters extracted from Jatropha oil. Of the pure phorbol esters tested, 4 beta-phorbol-13-decanoate killed both snail species at a concentration of 0.001% (10 p.p.m). As Jatropha is locally grown in Mali for other purposes, it might potentially be exploited for schistosomiasis control.

AN: 98132888

MEDLINE EXPRESS (R) 1998/01-1998/10 313 of 324

TI: Nerve growth factor (NGF) reduces and NGF antibody exacerbates retinal damage induced in rabbit by experimental ocular hypertension.

AU: Lambiase-A; Centofanti-M; Micera-A; Manni-GL; Mattei-E; De-Gregorio-A; de-Feo-G; Bucci-MG; Aloe-L

AD: CNR, Institute of Neurobiology, Rome, Italy.

SO: Graefes-Arch-Clin-Exp-Ophthalmol. 1997 Dec; 235(12): 780-5

This title is owned by this library

ISSN: 0721-832X

LA: ENGLISH

AB: BACKGROUND: It has been shown that intravitreal injection of NGF inhibits ganglion cell degeneration after optic nerve transection and ischemic injury. The aim of our study was to investigate the presence of NGF in aqueous humor and its involvement in retinal damage during ocular hypertension. METHODS: We used an experimental model of ocular hypertension in rabbit. Before treatment and 4, 10 and 15 days after induction of hypertension, we evaluated histological retinal damage and NGF levels in aqueous humor using an immunoenzymatic assay. Polyclonal anti-NGF antibodies were injected intravitreally into one eye of each rabbit (n = 6), and the animals were killed after 4 days of hypertension. Another group of rabbits (n = 12) was injected retro-ocularly with NGF and killed after 10 or 15 days of treatment for histologic evaluation of the retina. RESULTS: Our data show that experimental ocular hypertension in adult rabbits induces retinal damage and enhances local NGF levels. The highest NGF value was found after 4 days of intraocular hypertension: high levels persisted, though to a lesser extent, for up to 15 days. Histological examination revealed that the number of retinal ganglion cells (RGC) remained unchanged during the first 4 days but decreased at 10 days. These studies also showed that retro-ocular administration of NGF reduced RGC loss, whereas intraocular injection of NGF antibodies, which inhibited the endogenous NGF, exacerbated the retinal insult. CONCLUSION: These findings demonstrate a protective effect of NGF on RGC damaged by ocular hypertension and prompt further investigations to evaluate a possible therapeutic use of NGF to retard RGC death in humans.

AN: 98103145

MEDLINE EXPRESS (R) 1998/01-1998/10 314 of 324

TI: Role of TNF-alpha but not NGF in murine hyperalgesia induced by parasitic infection.

AU: Aloe-L; Moroni-R; Angelucci-F; Fiore-M

AD: Institute of Neurobiology, CNR, Rome, Italy.

SO: Psychopharmacology-Berl. 1997 Dec; 134(3): 287-92

This title is owned by this library

ISSN: 0033-3158

LA: ENGLISH

AB: Using adult mice infected with the trematode Schistosoma mansoni, we observed that this infection induces both thermal hyperalgesia and an increase in the levels of nerve growth factor in the paws. To explore the mechanism involved in peripheral hypersensitivity during chronic infection, mice were infected with 60 cercariae of S. mansoni and injected 17 weeks later with nerve growth factor, anti-nerve growth factor or with other molecules known to be associated with hyperalgesic processes. The results of these studies showed that antibodies against tumor necrosis factor-alpha, but not against nerve growth factor, reduce thermal sensitivity in schistosome infected mice, suggesting that this cytokine but not NGF plays a crucial role in schistosome-induced thermal hyperalgesia. Treatments with anti-inflammatory drugs support this hypothesis.

AN: 98099516

MEDLINE EXPRESS (R) 1998/01-1998/10 315 of 324

TI: Solid persistent facial oedema (Morbihan's disease) following rosacea, successfully treated with isotretinoin and ketotifen [letter]

AU: Mazzatenta-C; Giorgino-G; Rubegni-P; de-Aloe-G; Fimiani-M

SO: Br-J-Dermatol. 1997 Dec; 137(6): 1020-1

This title is owned by this library

ISSN: 0007-0963

LA: ENGLISH

AN: 98131709

MEDLINE EXPRESS (R) 1998/01-1998/10 316 of 324

TI: NGF content and expression in the rat pituitary gland and regulation by thyroid hormone.

AU: Calza-L; Giardino-L; Aloe-L

AD: Institute for Human Physiology, University of Cagliari, Italy. cefisnmo@mbox.vol.it

SO: Brain-Res-Mol-Brain-Res. 1997 Nov; 51(1-2): 60-8

ISSN: 0169-328X

LA: ENGLISH

AB: The involvement of nerve growth factor (NGF) in neuroendocrine regulation is supported by several lines of evidence. In this paper, we investigated the NGF content and expression in the pituitary gland and other endocrine organs during dysendocrine states (thyroidectomized, adrenalectomized and gonadectomized male rats). We found an increase of NGF-IR in the pituitary gland and testis of hypothyroid rats whereas no differences were found in the adrenal gland and blood. Also, NGF mRNA expression had increased in the anterior pituitary of hypothyroid rats whereas it had not changed after adrenalectomy and gonadectomy. Moreover, other neurotrophins and neurotrophin high-affinity receptors were unchanged in the anterior pituitary of hypothyroid rats. These data indicate that pituitary NGF is selectively modulated by thyroid status of the animal, further supporting a close link between NGF and thyroid hormone.

AN: 98087379

MEDLINE EXPRESS (R) 1998/01-1998/10 317 of 324

TI: Prevention of ultraviolet radiation-induced suppression of accessory cell function of Langerhans cells by Aloe vera gel components.

AU: Lee-CK; Han-SS; Mo-YK; Kim-RS; Chung-MH; Park-YI; Lee-SK; Kim-YS

AD: College of Pharmacy, Chungbuk National University, Cheongju, South Korea.

SO: Immunopharmacology. 1997 Oct; 37(2-3): 153-62

This title is owned by this library

ISSN: 0162-3109

LA: ENGLISH

AB: The active components of Aloe vera gel that can prevent ultraviolet B (UVB)-induced suppression of accessory cell function of Langerhans cells (LC) were purified by activity-guided sequential fractionation followed by in vitro functional assay. The functional assay was based on the fact that exposure of freshly isolated murine epidermal cells (EC) to UVB radiation resulted in impairment of accessory cell function of LC, as measured by their ability to support anti-CD3 monoclonal antibody (mAb)-primed T-cell mitogenesis. This UVB-suppressed LC accessory cell function was prevented by addition of partially purified Aloe gel components to cultures of UVB-irradiated EC. The Aloe gel components appeared to prevent events occurring within the first 24 h after UVB irradiation that lead to the impairment of accessory cell function. The Aloe gel components did not cause proliferation of anti-CD3 mAb-primed T-cells, nor did induce proliferation of normal EC. The activity-guided final purification of Aloe gel components resulted in the isolation of two components. Both of the components were small molecular weight (MW) substances with an apparent MW of less than 1,000 Da but different from each other in net charge characteristics at pH 7.4. These results suggest that Aloe vera gel contains at least two small molecular weight immunomodulators that may prevent UVB-induced immune suppression in the skin.

AN: 98067037

MEDLINE EXPRESS (R) 1998/01-1998/10 318 of 324

TI: Cytotoxicity of a low molecular weight fraction from Aloe vera (Aloe barbadensis Miller) gel.

AU: Avila-H; Rivero-J; Herrera-F; Fraile-G

AD: Centro de Investigaciones Biomedicas (BIOMED), Facultad de Ciencias de la Salud, Universidad de Carabobo, Maracay, Venezuela.

SO: Toxicon. 1997 Sep; 35(9): 1423-30

This title is owned by this library

ISSN: 0041-0101

LA: ENGLISH

AB: The cytotoxicity of a low mol. wt fraction (LMWF) obtained from Aloe vera gel was determined by two different assays. Firstly, exposure of monolayers of chicken fibroblasts to LMWF induced disruption of intercellular junctions and detachment of individual cells from the bottom of the flask, with formation of cell-free gaps in the monolayer. Secondly, LMWF inhibited the production of reactive oxygen species by human polymorphonuclear leukocytes stimulated by zymosan, as followed by luminol-dependent chemiluminescence. The toxic activity of LMWF was compared to that of sodium dodecyl sulfate (a well-known toxic substance), aloe-emodin and aloin (an anthraquinone and its precursor present in Aloe vera cortex) using the chemilumescence assay, and was found to be of similar potency to these toxic substances on a weight-to-weight basis. These results confirm that Aloe vera gel contains toxic low mol. wt compounds, and every effort must be made to limit the amount of these toxins in the commercially prepared Aloe vera gel products.

AN: 98067751

MEDLINE EXPRESS (R) 1998/01-1998/10 319 of 324

TI: Effect of the combination of Aloe vera, nitroglycerin, and L-NAME on wound healing in the rat excisional model.

AU: Heggers-JP; Elzaim-H; Garfield-R; Goodheart-R; Listengarten-D; Zhao-J; Phillips-LG

AD: University of Texas Medical Branch, Galveston, USA.

SO: J-Altern-Complement-Med. 1997 Summer; 3(2): 149-53

ISSN: 1075-5535

LA: ENGLISH

AB: PURPOSE: Many systemic and topical therapeutic agents such as growth hormone, platelet-derived growth factor (PDGF), fibroblast growth factor (FGF), epidermal growth factor (EGF), and insulin-like growth factor (IGF) have been used as vulnerary agents. However, the role of nitric oxide (NO) as a wound-healing stimulant has been received with mixed reviews. NO is a potent vasodilator that is thought to be an endothelium-dependent relaxing factor, and a regulator of blood pressure and regional blood flow. It affects vascular smooth muscle proliferation and inhibits platelet aggregation and leukocyte adhesion. Therefore we compared the effects of several topical substances that have similar or reverse properties. METHODS: Using the excisional rat wound model, we evaluated the topical effects of Dermaide Aloe (D-Aloe, Dermaide Research Corp, Palos Heights, IL), nitroglycerin, Aquaphor (Beuersdorf, Inc., Norwalk, CT) alone, with D-Aloe with nitroglycerin, 2%, and L-NAME (NO inhibitor) with Aquaphor, and L-NAME with Aquaphor and D-Aloe for a 21-day period. All wounds were measured by planimetry at 1, 7, 10, 13, 16, 18, and 21 days. RESULTS: At day 1, all wounds had an average wound size of 2.27 cm2 (SD +/- 0.372) with no significant difference in wound size among the groups. Topically applied D-Aloe appeared to promote wound healing faster than the remaining other topicals (p < .05, Student-Newman-Keuls and Dunn's Method) over the study period. However, topicals combined with D-Aloe, the vehicle Aquaphor, and L-NAME improved the wound healing process when compared with nitroglycerin alone (p < .05). CONCLUSIONS: D-Aloe appears to have a wound-healing advancement factor that can reverse the effects of petrolatum- and nitroglycerin-based products as observed in the remaining groups when compared with nitroglycerin alone. It appears that D-Aloe's effect of preventing dermal ischemia by reversing the effects of thromboxane synthetase (TxA2) may act synergistically with NO or could be an oxygen radical scavenger.

AN: 98051887

MEDLINE EXPRESS (R) 1998/01-1998/10 320 of 324

TI: Extracorporeal photochemotherapy restores Th1/Th2 imbalance in patients with early stage cutaneous T-cell lymphoma.

AU: Di-Renzo-M; Rubegni-P; De-Aloe-G; Paulesu-L; Pasqui-AL; Andreassi-L; Auteri-A; Fimiani-M

AD: Department of Immunology, University of Siena, Italy.

SO: Immunology. 1997 Sep; 92(1): 99-103

This title is owned by this library

ISSN: 0019-2805

LA: ENGLISH

AB: Extracorporeal photochemotherapy (ECP) has been shown to be a potent activator of peripheral blood macrophages because it causes a marked release of macrophage-dependent proinflammatory cytokines, and it is therefore currently considered to be a safe and non-toxic immunomodulatory treatment. On this basis we studied the function of peripheral blood mononuclear cells (PBMC) in eight patients with early stage (Ib) cutaneous T-cell lymphoma (CTCL), before and 1 year after ECP, together with their clinical and histological responses. In particular we evaluated in vitro phytohaemagglutinin (PHA)-stimulated proliferation and production of interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) as well as lipopolysaccharide (LPS)-induced production of IL-12. Before treatment we observed that PBMC of patients produced significantly higher levels of IL-4 and lower levels of IFN-gamma and IL-12 than those of healthy control subjects. After 1 year of ECP, IL-4, IFN-gamma and IL-12 production no longer differed from that of control subjects. Moreover, we observed a good clinical result matched by histological response. Our data confirm that early-stage CTCL patients show a predominantly type-2 immune response that might be responsible for several immunological abnormalities found in this disease. We have demonstrated that ECP reverses the T-helper type 1/T-helper type 2 (Th1/Th2) imbalance and may therefore be considered an efficient biological response modifier.

AN: 98038264

MEDLINE EXPRESS (R) 1998/01-1998/10 321 of 324

TI: Beneficial effect of Aloe on wound healing in an excisional wound model.

AU: Heggers-JP; Kucukcelebi-A; Listengarten-D; Stabenau-J; Ko-F; Broemeling-LD; Robson-MC; Winters-WD

AD: University of Texas Medical Branch, Galveston, USA.

SO: J-Altern-Complement-Med. 1996 Summer; 2(2): 271-7

ISSN: 1075-5535

LA: ENGLISH

AB: Recent evidence from in vitro and in vivo experiments suggests that topical antimicrobials may be toxic to fibroblasts and keratinocytes and retard wound healing. The purpose of this study was to determine the effects of Aloe, a potential wound-healing agent, on wound contraction in excisional wounds treated with topical antimicrobials. Sprague-Dawley rats were prepared with four 1.5 cm2 dorsal defects through the skin and panniculus. The animals were divided into five groups (n = 10 per group): (1) Aloe, (2) NaOCl solution (0.025%), (3) mafenide acetate, (4) mafenide acetate + Aloe, and (5) control. Wounds were treated topically for 14 days 3 times a day. Serial standard photographs and serial wound planimetry were performed weekly. Following healing, the breaking strength of each resultant scar was determined using an Instron tensiometer. Kruskal-Wallis, ANOVA, and multiple comparison methods were used for data analysis. Aloe and NaOCl solution significantly accelerated wound contraction (p < 0.05). In the mafenide acetate + Aloe group, contraction was similar to the control, whereas the mafenide acetate alone retarded wound healing. The addition of Aloe in combination and alone in wounds increased the breaking energy when compared to controls (p < 0.05). Aloe appears to expedite wound contraction and neutralize the wound retardant effect seen with the topical mafenide acetate alone. This effect appears to be due to an increased collagen activity, which is enhanced by a lectin, consequently improving the collagen matrix and enhancing the breaking strength.

AN: 98051842

MEDLINE EXPRESS (R) 1998/01-1998/10 322 of 324

TI: Thyroid hormone-induced plasticity in the adult rat brain.

AU: Calza-L; Aloe-L; Giardino-L

AD: Institute of Human Physiology, University of Cagliari, Italy.

SO: Brain-Res-Bull. 1997; 44(4): 549-57

This title is owned by this library

ISSN: 0361-9230

LA: ENGLISH

AB: It is well known that thyroid hormone plays a crucial role in the development and maturation of the nervous system. However, little is known about the role of thyroid hormone in the adult brain. In this short review we have dwelt on this point, with regard to the role of thyroid hormone on neuropeptide gene expression regulation in the paraventricular nucleus of the hypothalamus and in extrahypothalamic brain areas, on neurotrophin and neurotrophin receptor expression in the hippocampus and basal forebrain in basal conditions, and after neurotoxic challenges. Effects of hypothyroidism are discussed in view of a possible role of thyroid status in brain aging quality.

AN: 98035969

MEDLINE EXPRESS (R) 1998/01-1998/10 323 of 324

TI: Extracorporeal photochemotherapy induces apoptosis of infiltrating lymphoid cells in patients with mycosis fungoides in early stages. A quantitative histological study.

AU: Miracco-C; Rubegni-P; De-Aloe-G; D'Ascenzo-G; Mazzatenta-C; De-Santi-MM; Fimiani-M

AD: Institute of Pathology, University of Siena, Italy.

SO: Br-J-Dermatol. 1997 Oct; 137(4): 549-57

This title is owned by this library

ISSN: 0007-0963

LA: ENGLISH

AB: Extracorporeal photochemotherapy (ExP) is a well-tolerated new form of chemoimmunotherapy, which is considered to be effective for cutaneous T-cell lymphoma (CTCL) and the treatment of choice for Sezary syndrome. Improvements have also been seen in patients with non-erythrodermic mycosis fungoides (MF) in the early stages, even when tumour cells are not detectable in the peripheral blood. In this study, we used ExP as a monotherapy in seven patients who had early stage (Ib) MF, and who were no longer responsive to or had contraindications for other therapies. We observed a clinical improvement in the disease after 12 months of treatment: one patient showed a complete response, five a partial response, and one remained stable. In each patient we compared skin biopsies of large plaque lesions before and after the treatment. We undertook a histological evaluation of the infiltrate. The lymphoid cell proliferation and death rates were quantified using the following parameters; lymphoid cell density (LCD), Ki67 + lymphoid cell nuclei percentage (Ki67 + Lcn percentage), and apoptotic index (AI). Significant decreases in the lymphoid cell infiltrate and in cell proliferation, and a significant increase in AI were observed after therapy. The mean LCD decreased from 187 +/- 33 to 34 +/- 17.7, Ki67 + Lcn mean percentage decreased from 16.9 +/- 3.9 to 4.9 +/- 2.4, and the AI mean value increased from 0.05 +/- 0.03 to 2.41 +/- 1.54. Our results suggest a role for apoptosis in the improvement of the skin lesions and are in line with some reports on the mode of action of ExP. Although the way in which ExP works needs to be clarified further, it does seem to stimulate a CD8+ cell-mediated anticlonotypic activity against circulating pathogenic clones. Furthermore, a release of tumour necrosis factor alpha (TNF-alpha) by circulating monocytes has been demonstrated after ExP. Both are known to induce cell death by apoptosis.

AN: 98052032